Effect of Quinolones on Blood Glucose Level and Blood Profile of Laying Hens

Abstract

A study was designed to investigate the effects of quinolones antibiotics on the blood profile, blood glucose level, histomorphological features of pancreas and egg production of laying hens. For this purpose, a total of 48 laying hens were selected and allocated into four groups, each group included three replicates containing four birds in each. Three commonly used quinolones including enrofloxacin, ciprofloxacin and norfloxacin were used for four weeks with one week of adaptation period, while, one group served as negative control. Blood was collected directly from wing vein and serum was analyzed for estimation of blood proteins, glucose level, cholesterol and albumin which showed non-significant (P>0.05) response to quinolones treatment. Highest blood glucose level was measured for control (239.21 ± 5.26), followed by groups treated with enrofloxacin (233.54± 4.19), ciprofloxacin (227.76 ±4.71) and lowest in norfloxacin (225.47 ± 4.85). Highest cholesterol level was measured in groups administered with norfloxacin (164.17± 3.55), followed by control (163.36±1.99), ciprofloxacin (161.53±3.14) and enrofloxacin (160.78± 3.18). The highest level of total protein in blood was recorded in control group (18.51±0.18), followed by enrofloxacin (18.43± 0.27), norfloxacin (18.28± 0.10) and lowest in ciprofloxacin treated group (18.12± 0.36). The highest level of albumin in blood was found in control group (2.82±0.19), followed by enrofloxacin (2.70±0.15), ciprofloxacin (2.61±0.06) and norfloxacin (2.54±0.12). The highest count of WBCs (white blood cells) in blood was recorded for group treated with ciprofloxacin (18.6 ± 10.63), followed by norfloxacin (17.6± 3.63) and control group (17.1± 10.63), while, lowest in enrofloxacin treated group (16.9± 2.53). Total RBCs (red blood cells) and average hemoglobin values showed non-significant (P>0.05) changes in blood profile of laying hens in the treated groups. Hens egg production showed significant (P < 0.05) increase in groups treated with different quinolones i.e., 10.95% was in norfloxacin, which was higher than control group followed by group treated with ciprofloxacin (8.63%) and enrofloxacin (6.5%). Histomorphology of pancreas treated with quinolones were noted and findings revealed that quinolones did not affect the histological architecture of the pancreas. It was thus concluded that quinolones have non-significant effect (P>0.05) on the serum biochemistry, blood profile and histomorphology of laying hens.

The Impact of Using Yeast or Tylosin as Growth Promoters on the Productive Performance and Physiological Characteristics of Broiler Quail

“…Also, adding Tylosin at levels of 0.1, 0.2, or 0.3 g/kg to the diet of quail chicks led to a significant reduction in the concentrations of serum total protein and albumin, compared to the control group which had the highest values. This confirms the findings of [31], who found that the blood albumin levels of laying hens were not significantly affected (P>0.05) by the administration of quinolone antibiotics.…”

“…The birds that were fed a diet with 0.3 g Tylosin /kg of the diet had the lowest levels of total cholesterol, while the birds given a diet with 0.1 g Tylosin /kg had the lowest levels of triglycerides, with the highest levels observed in the control group. This result not agree with [31] who noted that the impact of quinolones on cholesterol levels was observed to be statistically non-significant (P>0.05). However, [33] found that broiler chickens that received 15 g Polyalthia longifolia /L of the water had a significantly higher level of cholesterol compared to the control group.…”

Genus Spodoptera (Hadeninae: Noctuidae: Lepidoptera): A New Species from Southern Punjab, Pakistan

“…This is a concern since the previous studies have reported the prevalence of multi-resistant Salmonella in animal food between 54.8% to 69.3% (Kamboh et al, 2018;Mikanatha et al, 2010). The high prevalence rates of MDR organisms in animal-origin food have been linked with antimicrobial agents such as tetracycline, penicillin and aminoglycoside which are been used frequently in animal husbandry (Ariffin et al, 2019;Rehman et al, 2019) and is therefore recognized as giant challenge in the veterinary and medical sciences to treat Salmonella infections (Thai et al, 2012).…”