Effect of race-specific elicitors ofCladosporium fulvumon the tomato plasma membrane Ca2+-ATPase

Lam, C. Y. Teresa; Xing, T.; Higgins, Verna J.; Blumwald, Eduardo

doi:10.1006/pmpp.1998.0152

Cited by 12 publications

(5 citation statements)

References 45 publications

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“…The rapid transient decrease in 45 Ca 2+ efflux observed in the presence of OG might reflect either dilution of cytosolic 45 Ca 2+ due to the increase in unlabelled Ca 2+ influx or a true inhibition of Ca 2+ extrusion driven by the PM Ca 2+ -ATPase. Inhibition of the tomato PM Ca 2+ -ATPase by race-specific elicitors has been reported by Lam et al (1998); unfortunately, we were not able to test by biochemical assays whether OG also inhibit PM Ca 2+ -ATPase during the first few minutes. Overall, the observed rapid effects of OG on PM Ca 2+ fluxes may contribute to a rapid increase in (Ca 2+ ) cyt , as observed in different plant materials in response to OG (Messiaen et al, 1993;Messiaen and Van Cutsem, 1994;Chandra and Low, 1997;Lecourieux et al, 2002;Navazio et al, 2002), which is required for onset of the oxidative burst (Chandra and Low, 1997).…”

Section: Discussionmentioning

confidence: 78%

Involvement of the Plasma Membrane Ca²⁺‐ATPase in the Short‐Term Response of Arabidopsis thaliana Cultured Cells to Oligogalacturonides

et al. 2004

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Treatment of Arabidopsis thaliana cells with oligogalacturonides (OG) initiates a transient production of reactive oxygen species (ROS), the concentration of which in the medium peaks after about 20 min of treatment. The analysis of OG effects on Ca (2+) fluxes shows that OG influence both Ca (2+) influx and Ca (2+) efflux (measured as (45)Ca (2+) fluxes) in a complex way. During the first 10 - 15 min, OG stimulate Ca (2+) influx and decrease its efflux, while at successive times of treatment, OG cause an increase of Ca (2+) efflux and a slight decrease of its influx. Treatment with sub- micro M concentrations of eosin yellow (EY), which selectively inhibits the Ca (2+)-ATPase of plasma membrane (PM), completely prevents the OG-induced increase in Ca (2+) efflux. EY also suppresses the transient feature of OG-induced ROS accumulation, keeping the level of ROS in the medium high. The biochemical analysis of PM purified from OG-treated cells indicates that treatment with OG for 15 to 45 min induces a significant decrease in Ca (2+)-ATPase activation by exogenous calmodulin (CaM), and markedly increases the amount of CaM associated with the PM. During the same time span, OG do not influence the expression of At-ACA8, the main isoform of PM Ca (2+)-ATPase in suspension-cultured A. thaliana cells, and of CaM genes. Overall, the reported results demonstrate that the PM Ca (2+)-ATPase is involved in the response of plant cells to OG and is essential in regulation of the oxidative burst.

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Section: Discussionmentioning

confidence: 78%

Involvement of the Plasma Membrane Ca²⁺‐ATPase in the Short‐Term Response of Arabidopsis thaliana Cultured Cells to Oligogalacturonides

et al. 2004

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“…Abscisic acid (ABA) accumulation, a typical response in many plants subjected to drought, stimulated the increase in cytosolic Ca 2+ by inducing both Ca 2+ influx from the extracellular space and Ca 2+ release from intracellular stores (Pei et al 2000, Murata et al 2001. Cytosolic Ca 2+ efflux is regulated by Ca 2+ -ATPase (Lam et al 1998), implying that Ca 2+ -ATPase activity must be increased to pump excess cytosolic Ca 2+ into extracellular space and/or vacuole. In the present work, therefore, the increased Ca 2+ -ATPase activity under mild drought (0-6 d) may result from the stimulation of excess cytosolic Ca 2+ concentration.…”

Section: Discussionmentioning

confidence: 99%

Effects of drought on photosynthetic characteristics of flag leaves of a newly-developed super-high-yield rice hybrid

Chen¹,

Liu²,

Zhang³

et al. 2004

Photosynt.

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We investigated responses of chloroplasts from flag leaves of a newly-developed super-high-yield rice (Oryza sativa L.) hybrid LiangYouPeiJiu (LYPJ) to water stress (withholding irrigation) during the grain-filling period. In the early stage of water stress (0-6 d) only the activity of Hill reaction was inhibited, whereas activities of photophosphorylation and Ca 2+ -ATPase, and ATP content were increased and peaked in the day 6 of withholding irrigation. In the late stage of water stress (6-12 d), the activities of photosynthetic O 2 evolution, Hill reaction, photophosphorylation, and Ca 2+ -ATPase, and ATP content were significantly reduced. The membrane lipid content was sharply decreased, especially of sulfoquinovosyl-diacylglycerol (SQDG) and phosphatidylglycerol (PG). The changes in the ultrastructure of chloroplasts included mainly a decrease in number of grana and increase in number of osmiophilic granules.

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“…Tomato ( Lycopersicon esculentum cv Moneymaker) and tobacco ( Nicotiana tabacum cv Petit Havana SR1) cell‐suspension cultures were grown as described previously (Parsons et al ., 1999; Vera‐Estrella et al ., 1992). Soluble, microsomal and plasma membrane cell fractions were prepared as described by Lam et al . (1998), with the exception that 5 m m EGTA and 5% w/v insoluble polyvinylpolypyrrolidone were included in the homogenization buffer.…”

Section: Methodsmentioning

confidence: 99%

“…Tomato cell extracts were prepared as described previously (Lam et al ., 1998), centrifuged at 10 000 g for 20 min, and the supernatant fraction (termed cell homogenate) used for recruitment experiments. Aliquots (20 µl, containing approximately 40 µg total protein) were incubated for 20 min at room temperature in the presence of either 5 m m EGTA, 10 µ m CaCl 2 and 50 µ m trifluoperazine (TFP), 10 µ m CaCl 2 alone, or 10 µ m CaCl 2 and 1 µ m recombinant petunia CaM81 with gentle shaking.…”

Section: Methodsmentioning

confidence: 99%

Alternative splicing of a novel diacylglycerol kinase in tomato leads to a calmodulin‐binding isoform

Snedden

Blumwald²

2000

The Plant Journal

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Summary Calmodulin is a regulatory protein activated during Ca2+ signalling. We have isolated a cDNA, designated LeCBDGK (Lycopersicon esculentum calmodulin‐binding diacylglycerol kinase) encoding a novel calmodulin‐binding protein with sequence similarity to diacylglycerol kinases from animals. Diacylglycerol kinases convert diacylglycerol to phosphatidic acid. We delineated the calmodulin‐binding domain to approximately 25 residues near the C‐terminus of LeCBDGK. We have also isolated a second diacylglycerol kinase cDNA, designated LeDGK1, identical to LeCBDGK, except that it lacks the calmodulin‐binding domain. Both recombinant LeCBDGK and LeDGK1 were catalytically active in vitro. Anti‐DGK antiserum detected two immunoreactive proteins associated with microsomal and plasma membrane fractions from cell suspensions. The higher molecular weight immunoreactive protein was also present in soluble extracts and bound to calmodulin–agarose in the presence of calcium, demonstrating that native LeCBDGK is a calmodulin‐binding protein. In the presence of calcium, LeCBDGK associated with membrane cell fractions in vitro, but calmodulin antagonists disrupted this association, suggesting a possible role of calcium in the recruitment of LeCBDGK from soluble to membrane cell fractions. Native LeCBDGK and calmodulin co‐immunoprecipitated from tomato soluble cell extracts, suggesting their interaction in vivo. The same gene encodes both LeCBDGK and LeDGK1 and the calmodulin‐binding domain of LeCBDGK is encoded by a separate exon. Thus, alternative transcript splicing leads to calmodulin‐binding and non‐binding forms of diacylglycerol kinases in tomato. Possible roles of LeCBDGK and LeDGK1 in calcium and lipid signalling are discussed.

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Effect of race-specific elicitors ofCladosporium fulvumon the tomato plasma membrane Ca2+-ATPase

Cited by 12 publications

References 45 publications

Involvement of the Plasma Membrane Ca²⁺‐ATPase in the Short‐Term Response of Arabidopsis thaliana Cultured Cells to Oligogalacturonides

Involvement of the Plasma Membrane Ca²⁺‐ATPase in the Short‐Term Response of Arabidopsis thaliana Cultured Cells to Oligogalacturonides

Effects of drought on photosynthetic characteristics of flag leaves of a newly-developed super-high-yield rice hybrid

Alternative splicing of a novel diacylglycerol kinase in tomato leads to a calmodulin‐binding isoform

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Effect of race-specific elicitors ofCladosporium fulvumon the tomato plasma membrane Ca2+-ATPase

Cited by 12 publications

References 45 publications

Involvement of the Plasma Membrane Ca2+‐ATPase in the Short‐Term Response of Arabidopsis thaliana Cultured Cells to Oligogalacturonides

Involvement of the Plasma Membrane Ca2+‐ATPase in the Short‐Term Response of Arabidopsis thaliana Cultured Cells to Oligogalacturonides

Effects of drought on photosynthetic characteristics of flag leaves of a newly-developed super-high-yield rice hybrid

Alternative splicing of a novel diacylglycerol kinase in tomato leads to a calmodulin‐binding isoform

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Involvement of the Plasma Membrane Ca²⁺‐ATPase in the Short‐Term Response of Arabidopsis thaliana Cultured Cells to Oligogalacturonides

Involvement of the Plasma Membrane Ca²⁺‐ATPase in the Short‐Term Response of Arabidopsis thaliana Cultured Cells to Oligogalacturonides