Effect of radiation-induced damage of trabecular bone tissue evaluated using indentation and digital volume correlation

Full-Field Strain Measurements of the Muscle-Tendon Junction Using X-ray Computed Tomography and Digital Volume Correlation

“…Using our PTA staining protocol, the results presented illustrated the mechanical behavior across the MTJ. Even larger gaps between the muscle fibers and tendon fascicles were detected on the PTA-stained specimens after XCT imaging than on the stained specimen without XCT imaging (Figure 6g), and this suggested that the imaging process could have affected the tissue further through dehydration or radiation damage [54]. Even though XCT has this limitation, it has advantages over other imaging techniques that have been used to analyze strain distribution.…”

“…Synchrotron has higher resolution than XCT; however, it also has higher beam current [37] and has been reported to damage biological macromolecules, which affects the morphology and mechanical properties of the specimens [75,76]. It has been reported previously that the temperature inside the XCT increases by only 2 • C after 3-h XCT imaging [54]. Therefore, the damage on the specimens through the XCT imaging process may be less than the other imaging techniques.…”

“…Phase contrast XCT is useful for soft tissue imaging [52], however, it requires a longer scan time than XCT without phase contrast to acquire sufficient number of projections [53], which can also damage specimens. A previous study on trabecular bone showed that 9-h XCT imaging did not cause any radiation damage on specimens [54]. Therefore, XCT was chosen as an imaging technique to be used for the strain measurement in this study.…”

Data-driven image mechanics (D2IM): a deep learning approach to predict displacement and strain fields from undeformed X-ray tomography images - Evaluation of bone mechanics

“…higher resolution) and mechanical (i.e. increased number of in situ steps) requirements are more demanding, with experiment duration for one specimen close or exceeding the 24h [3, 4]. This can result in extended exposure to X-rays, which is proven to have a substantial impact on the structural and mechanical properties of musculoskeletal tissues both in high-flux synchrotron [5] and laboratory XCT setups [3].…”

“…increased number of in situ steps) requirements are more demanding, with experiment duration for one specimen close or exceeding the 24h [3, 4]. This can result in extended exposure to X-rays, which is proven to have a substantial impact on the structural and mechanical properties of musculoskeletal tissues both in high-flux synchrotron [5] and laboratory XCT setups [3]. In addition, experimental campaigns to characterise bone using in situ XCT mechanics and DVC are typically conducted with a limited sample size of 2-3 specimens per bone type due to tissue availability, particularly in cases of in vivo treatment [6].…”