Effect of Radiation Sterilization on the Osteoinductive Properties and the Rate of Remodeling of Bone Implants Preserved by Lyophilization and Deep-Freezing

Dziedzic‐Goclawska, A.; Ostrowski, K.; Stachowicz, W.; Michalik, J.; Grzesik, Wojciech J.

doi:10.1097/00003086-199111000-00006

Cited by 50 publications

(31 citation statements)

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“…Freezing reduces the mobility of water molecules, and may therefore decrease the production of free radicals. Protection of the osteoinductive properties of bone allografts has been observed when irradiation was performed at low temperatures (Dziedzic-Goclawska et al 1991). Furthermore, the removal of marrow by rinsing the allografts before irradiation may have been favorable, since gamma irradiation of human bone allografts alters medullary lipids and releases toxic compounds from osteoblastlike cells (Moreau et al 2000).…”

Section: Histomorphometrymentioning

confidence: 99%

“…These proteins may be released during the resorptive phase of bone graft incorporation, and can have a profound effect on bone cell physiology. Although studies have been performed to determine the effect of irradiation on BMPs (Munting et al 1988, Wientroub and Reddi 1988, Dziedzic-Goclawska et al 1991, Ijiri et al 1994, Andriano et al 2000, Ripamonti et al 2000, Pekkarinen et al 2005, the effect of irradiation on the incorporation process of morselized bone chips remains unclear. We studied the effects of steri-lization by gamma irradiation on the incorporation of impacted morselized allografts.…”

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confidence: 99%

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Irradiation has no effect on the incorporation of impacted morselized bone: A bone chamber study in goats

2007

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Background Gamma irradiation has been widely used for sterilization of bone allografts. However, gamma irradiation alters proteins. This is favorable when it reduces immunogenicity, but is undesirable when osteoinductive proteins are damaged. Although the effect of gamma irradiation on BMPs has been studied, the effect of irradiation on the process of incorporation of morselized bone chips remains unclear. We studied the effects of sterilization by gamma irradiation on the incorporation of impacted morselized allografts.Methods Bone chambers with impacted allografts, rinsed impacted allografts, allografts that were rinsed and subsequently irradiated, and an empty control were implanted in proximal medial tibiae of goats. Incorporation was evaluated using histology and histomorphometry.Results Histology revealed evidence of bone graft incorporation, which proceeded in a similar way in unprocessed, rinsed, and both rinsed and irradiated bone grafts. After 12 weeks, no difference in bone and tissue ingrowth was found between the unprocessed, the rinsed, and the rinsed and subsequently irradiated allografts. The amount of unresorbed graft remnant was highest in the unprocessed bone grafts.Interpretation We conclude that sterilization with gamma irradiation does not influence the incorporation of impacted rinsed bone allografts.

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Section: Histomorphometrymentioning

confidence: 99%

mentioning

confidence: 99%

Irradiation has no effect on the incorporation of impacted morselized bone: A bone chamber study in goats

2007

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“…The increased damages observed in absence of free water in pre-dried specimen may be due to direct damages to the proteins by irradiation, suggesting a higher sensitivity of freezedried proteins to irradiation than hydrated ones. This is supported by the observation of better osteoinductive properties of demineralised powder when irradiated in the hydrated frozen state (54). Collagen degradation by irradiation may account for the accelerate graft remodelling (54-55).…”

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confidence: 94%

Influence of Freeze-Drying and Irradiation on Mechanical Properties of Human Cancellous Bone: Application to Impaction Bone Grafting

Cornu

2012

Bone Grafting

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“…17,20 g-Irradiation is a widely accepted secondary sterilization procedure. 15,[21][22][23][24][25] Six hundred thousand tissue grafts, a conservative estimate figure, in developed countries are sterilized annually using radiation. 26 A variable radiation doses (10-50 kGy) has been used to treat allogenic bone grafts.…”

Section: Introductionmentioning

confidence: 99%

BMP‐7 and CBFA1 in allograft bone in vivo bone formation and the influence of γ‐irradiation

Chen

Yang

et al. 2006

J Biomedical Materials Res

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An initial study showed that morselized human bone grafts were osteoconductive and osteoinductive when implanted in nude rat tibial window defects, and 25 kGy of gamma-irradiation significantly reduced those properties. The mechanism of the osteoinductivity and the influence of gamma-irradiation required further investigation. In this study we assessed the paraffin sections of seven morselized human bone grafts implanted into rat tibial defects for 3 weeks after being treated with 0, 15, or 25 kGy gamma-radiation respectively. Osteoclast-like cell counting and protein expressions of bone morphogenetic protein-7 (BMP-7), core binding factor alpha1 (CBFA1), and proliferating cell nuclear antigen (PCNA) were investigated and the positive signals were quantitatively analyzed. More new bone formation was observed in the 0 and 15 kGy groups compared with 25 kGy groups. The newly formed bones were found mainly from the intact cortex into the defects bridged by the implanted grafts. A dense staining of BMP-7 and CBFA1 was noted in the osteoblast-like cells in those areas. The BMP-7 and CBFA1 staining was also seen in the cells surrounding the implanted grafts in the centre areas of the defects in distance from the intact cortex. Quantitative analysis of immunohistochemical staining of the centre areas of the defects showed that gamma-irradiation (15 and 25 kGy) significantly reduced the expression of CBFA1 and BMP-7. In conclusion, morselized human bone grafts may contain some factors, which induced osteoblast lineage differentiation and bone formation and gamma-irradiation damages those bone inducing factors.

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Effect of Radiation Sterilization on the Osteoinductive Properties and the Rate of Remodeling of Bone Implants Preserved by Lyophilization and Deep-Freezing

Cited by 50 publications

References 0 publications

Irradiation has no effect on the incorporation of impacted morselized bone: A bone chamber study in goats

Irradiation has no effect on the incorporation of impacted morselized bone: A bone chamber study in goats

Influence of Freeze-Drying and Irradiation on Mechanical Properties of Human Cancellous Bone: Application to Impaction Bone Grafting

BMP‐7 and CBFA1 in allograft bone in vivo bone formation and the influence of γ‐irradiation

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