Effect of Radiocontrasts on Selected Membrane Transport Systems

Oliveira, Rubens Marona de; Costa, Bartira Ercí­lia Pinheiro da; Fervenza, Fernando C.; Wagner, Mário Bernardes; d'Avila, Domingos O.; Poli-de-Figueiredo, Carlos Eduardo

doi:10.1080/08860220500243247

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“…Modifications of carrier-mediated membrane transport have been described in pregnancy [2,3], uremia [4][5][6][7][8][9][10], hypertension [11], pregnancy-induced hypertension [12][13][14], and inherited diseases [1]. The hypothesis of this study is that cell incubation at physiological temperature (37°C) depletes the existing intracellular arginine pool and alters membrane transporters kinetic parameters.…”

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confidence: 99%

Erythrocytes l-Arginine y+ Transporter Inhibition by N-Ethylmaleimide in Ice-bath

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et al. 2010

Cell Biochem Biophys

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Erythrocytes L: -arginine uptake is conveyed by y+ and y+L membrane transport systems. Pre-incubation with N-ethylmaleimide for 10 min at 37°C inhibits the y+ system. The aim of this study was to determine the ideal pre-incubation temperature in evaluating y+ and y+L systems. Cells were pre-incubated with or without N-ethylmaleimide for 10 min at 4°C and 37°C. L: -Arginine uptake was quantified by radioisotope and standard erythrocytes membrane flux methodology. Results demonstrate that erythrocytes L: -arginine content is depleted by pre-incubation at 37°C for 10 min, thus changing the V (max) measurement. The inhibitory effect of N-ethylmaleimide pre-incubation was temperature independent and already complete after 1 min of incubation. No significant difference in kinetic parameters was detected between cells pre-incubated at 37°C or 4°C, under zero-trans conditions. In conclusion, we suggest that measurement of erythrocytes L: -arginine uptake by y+ and y+L systems could be carried out without N-ethylmaleimide pre-incubation at 37°C.

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