Effect of radiotherapy on the DNA cargo and cellular uptake mechanisms of extracellular vesicles

Szatmári, Tünde; Balázs, Katalin; Csordás, Ilona Barbara; Sáfrány, Géza; Lumniczky, Katalin

doi:10.1007/s00066-023-02098-2

Cited by 5 publications

(8 citation statements)

References 242 publications

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“…Based on their size and biogenetic pathways, EVs can be delineated into three distinct types: exosomes, microvesicles, and apoptotic bodies (ABs) [ 4 , 17 , 21 ]. Each subtype of EV possesses unique characteristics and functions in intercellular communication.…”

Section: Formation Of Evs and Mechanisms Of Exodna Loadingmentioning

confidence: 99%

“…Recently, a new biopsy method called liquid biopsy (LB) has gained attention for its ability to detect various fluid components and screen for early stage tumors and evaluate drug efficacy. The main materials used in LB for tumor diagnosis are tumor circulating cells (CTCs), circulating tumor DNA (ctDNA), and extracellular vesicles (EVs), which contain tumor-specific biomarkers such as DNA driver mutations, miRNA, and cell molecules [ 1 , 2 , 3 , 4 , 5 ]. However, there are some issues with ctDNA.…”

Section: Introductionmentioning

confidence: 99%

“…As a result, disease diagnosis and treatment may be delayed until ctDNA secretion reaches a detectable level, which typically occurs in the advanced stages of a tumor. Therefore, the effective acquisition and detection of tumor-derived DNA is an area of focus [ 3 , 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…They play a critical role in intercellular communication, as well as tumor growth, migration, and recurrence [ 1 , 9 ]. EVs carry various bioactive molecules, including DNA, miRNA, lipids, and proteins, and their genetic characteristics are consistent with the parental cells, reflecting the physiological and pathological status of the body [ 2 , 3 , 4 ]. Of the various molecules, exosomal DNA (exoDNA) is particularly noteworthy, comprising nuclear DNA, mitochondrial DNA (mtDNA), and even viral DNA [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…Cancers 2024, 16, x FOR PEER REVIEW 2 of 20 molecules [1][2][3][4][5]. However, there are some issues with ctDNA.…”

Section: Introductionmentioning

confidence: 99%

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Exosomal DNA: Role in Reflecting Tumor Genetic Heterogeneity, Diagnosis, and Disease Monitoring

Xiang,

Xie,

2023

Cancers

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Extracellular vesicles (EVs), with exosomes at the forefront, are key in transferring cellular information and assorted biological materials, including nucleic acids. While exosomal RNA has been thoroughly examined, exploration into exosomal DNA (exoDNA)—which is stable and promising for cancer diagnostics—lags behind. This hybrid genetic material, combining contributions from both nuclear and mitochondrial DNA (mtDNA), is rooted in the cytoplasm. The enigmatic process concerning its cytoplasmic encapsulation continues to captivate researchers. Covering the entire genetic landscape, exoDNA encases significant oncogenic alterations in genes like TP53, ALK, and IDH1, which is vital for clinical assessment. This review delves into exosomal origins, the ins and outs of DNA encapsulation, and exoDNA’s link to tumor biology, underscoring its superiority to circulating tumor DNA in the biomarker arena for both detection and therapy. Amidst scientific progress, there are complexities in the comprehension and practical application of the exoDNA surface. Reflecting on these nuances, we chart the prospective research terrain and potential pitfalls, forging a path for future inquiry. By illuminating both the known and unknown facets of exoDNA, the objective of this review is to provide guidance to the field of liquid biopsy (LB) while minimizing the occurrence of avoidable blind spots and detours.

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Section: Formation Of Evs and Mechanisms Of Exodna Loadingmentioning

confidence: 99%