Effect of rainbow trout (Oncorhynchus mykiss) seminal plasma on the post-thaw quality of ram semen cryopreserved in a soybean lecithin-based or egg yolk-based extender

Üstüner, Burcu; Alçay, Selim; Toker, Mehmed Berk; Nur, Zekariya; Gökçe, Elif; Sonat, Füsun Ak; Gül, Zülfiye; Duman, Muhammed; Ceniz, Cafer; Uslu, Aydın; Sağırkaya, Hakan; Soylu, Mustafa Kemal

doi:10.1016/j.anireprosci.2015.11.017

Cited by 36 publications

(33 citation statements)

References 34 publications

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“…The acrosomal integrity is adversely affected by cryopreservation, and this adverse effect leads to fertilisation losses (Alcay, Gokce, et al, 2016; Nur et al, 2010; Ustuner et al, 2016). In our study, there was no statistical difference among RTS groups (RTS1, RTS2 and RTS4) and the control group at post‐thaw.…”

Section: Discussionmentioning

confidence: 99%

“…However, cryopreservation can induce detrimental effects (ice crystallisation, cold shock, lipid peroxidation, etc; Nur, Zik, Ustuner, Sagirkaya, & Ozguden, 2010). These undesirable effects may cause irreversible decrease on sperm function parameters (Nur et al, 2010; Ustuner et al, 2016). The success of sperm cryopreservation depends on the composition of the extenders which is a crucial factor to protect spermatozoon (Alcay, Gokce, et al, 2016; Toker, Alcay, Gokce, & Ustuner, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Seminal plasma contains biochemical components that regulate sperm function (Ciereszko, Glogowski, & Dabrowski, 2000). The use of seminal plasma of different species in the semen extenders has been tested to minimise the detrimental effects of cryopreservation (Baran, Ak, Ileri, & Soylu, 2004; Gunay et al, 2006; Ustuner et al, 2016). Rainbow trout seminal plasma (RTS) contains proteins (2.1 ± 0.3 mg/ml), monosaccharides, amino acids, free fatty acids, phospholipids, vitamins and neutral lipids (Ciereszko et al, 2000; Shaliutina‐Kolešová et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Rainbow trout seminal plasma (RTS) contains proteins (2.1 ± 0.3 mg/ml), monosaccharides, amino acids, free fatty acids, phospholipids, vitamins and neutral lipids (Ciereszko et al, 2000; Shaliutina‐Kolešová et al, 2016). In addition, RTS supplemented extenders (egg yolk based) have been successfully used for ram semen cryopreservation (Ustuner et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Goat semen cryopreservation with rainbow trout seminal plasma supplemented lecithin‐based extenders

et al. 2020

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The objective of this study was to determine the optimum concentrations of rainbow trout seminal plasma (RTS) supplemented extenders for goat semen quality at post‐thaw and after incubation. Five sexually mature Saanen goat (Capra aegagrus hircus) were used for semen collection. Pooled semen was diluted with soybean lecithin‐based extender without RTS (control) or supplemented with different concentrations of RTS (1%, 2%, 4% or 8%), at a final concentration of 150 × 106 spermatozoon/ml. Sperm motility, plasma membrane functional integrity (HOST), damaged acrosome (PSA‐FITC), mitochondrial activity (rhodamine123) and DNA integrity (TUNEL) were evaluated. Spermatological parameters were evaluated at post‐thaw and after 6 hr incubation. RTS8 group preserved sperm motility, acrosomal integrity, plasma membrane functional integrity and mitochondrial function better than the control group (p < .05). The study demonstrated that RTS supplemented lecithin‐based extenders have useful effects on goat spermatozoa. In addition, the results of the current study represented the positive effect of using 8% RTS supplemented extender.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Goat semen cryopreservation with rainbow trout seminal plasma supplemented lecithin‐based extenders

et al. 2020

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“…The results of the current study are in agreement with the previous reports in dogs (Axnér & Lagerson ), rams (Ustuner et al . ), and bulls (Crespilho et al . ).…”

Section: Discussionmentioning

confidence: 99%

Effects of egg yolk and soybean lecithin on sperm quality determined by computer‐assisted sperm analysis and confocal laser scanning microscope in chilled canine sperm

Nguyen

Ponchunchoovong

Kupittayanant

et al. 2019

Veterinary Medicine & Sci

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The reduction of spermatozoa survival time is a major problem of canine chilled sperm for artificial insemination. The aim of the study was to improve the quality of canine chilled sperm during storage time. We therefore, evaluated the effects of eight treatments with different levels of soybean lecithin concentration (1, 3 and 5%) and egg yolk (20%) in Tris‐citric‐fructose or Tris‐citric‐fructose‐mineral salts extender on chilled canine sperm quality during 10 days of storage. The sperm motility was analysed by computer‐assisted sperm analysis (CASA), whereas plasma membrane integrity, acrosome membrane integrity and mitochondrial membrane potential parameters were determined using a fluorescent staining combination of propidium iodide (PI), Hoechst 33342 (H342), fluorescein isothiocyanate‐conjugated Pisum sativum agglutinin (FITC‐PSA) and 5,5′,6,6′‐tetrachloro‐1,1′,3,3′‐tetraethylbenzimidazolyl‐carbocyanine iodide (JC‐1) by confocal laser scanning microscope. The results showed that egg yolk was found to be better than soybean lecithin in Tris‐citric‐fructose or Tris‐citric‐fructose‐mineral salts extender for maintaining the quality of chilled canine sperm within 10 days of storage (P < 0.05). Although egg yolk in Tris‐citric‐fructose extender could maintain the motility better than other extenders, egg yolk in Tris‐citric‐fructose‐mineral salts extender was the highest in intact plasma membrane, intact acrosome membrane and high mitochondrial membrane potential (P < 0.05). In contrast, the sperm quality of soybean lecithin in Tris‐citric‐fructose‐mineral salts extender was lower than that of soybean lecithin in Tris‐citric‐fructose extender, and soybean lecithin 1% was greater than soybean lecithin 3% and 5% in plasma membrane integrity, acrosome membrane integrity and mitochondrial membrane potential (P < 0.05). In conclusion, soybean lecithin cannot replace egg yolk in Tris‐citric‐fructose or Tris‐citric‐fructose‐mineral salts extenders, and egg yolk in Tris‐citric‐fructose‐mineral salts extender is superior to other extenders in chilling canine sperm.

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The effects of supplementation of vitamin D to the egg‐yolk extender on cryopreservation of ram semen

Aktar,

Toker,

Koca

et al. 2024

Veterinary Medicine & Sci

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ObjectivesThis study aimed to examine the effects of supplementation of vitamin D to the egg‐yolk extender on characteristics of frozen–thawed ram semen.MethodsSemen samples obtained from adult rams were pooled and divided into five equal volumes. It was reconstituted with extenders containing different concentrations of vitamin D: 0 (control), 12.5 (VITD 12.5), 25 (VITD 25), 50 (VITD 50), and 100 ng/mL (VITD 100), and then they were frozen. Sperm motility parameters, plasma membrane functional integrity, acrosomal integrity, DNA fragmentation, and mitochondrial membrane potential of the groups were evaluated after sperm thawing.ResultsTotal motility and progressive motility were higher in VITD 50 than in all other groups (p < 0.05). Higher sperm straightness, linearity, and wooble were higher in VITD 50 than in the control group (p < 0.05). A similar pattern of VITD 50 was observed for plasma membrane integrity and mitochondrial membrane potential (p > 0.05).ConclusionsIn the study, it was observed that adding vitamin D to the extender had a beneficial effect on ram spermatological parameters. In addition, it was concluded that the use of the 50 ng/mL vitamin D in the extender provided more effective protection than the other doses.

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Effect of rainbow trout (Oncorhynchus mykiss) seminal plasma on the post-thaw quality of ram semen cryopreserved in a soybean lecithin-based or egg yolk-based extender

Cited by 36 publications

References 34 publications

Goat semen cryopreservation with rainbow trout seminal plasma supplemented lecithin‐based extenders

Goat semen cryopreservation with rainbow trout seminal plasma supplemented lecithin‐based extenders

Effects of egg yolk and soybean lecithin on sperm quality determined by computer‐assisted sperm analysis and confocal laser scanning microscope in chilled canine sperm

The effects of supplementation of vitamin D to the egg‐yolk extender on cryopreservation of ram semen

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