Effect of Rat Intrinsic Factor on Vitamin B12 Absorption in Pernicious Anemia

Abels, J.; Woldring, Mg; Vegter, J. J. M.; Nieweg, H. O.

doi:10.1126/science.126.3273.558

Cited by 9 publications

(3 citation statements)

References 2 publications

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“…The activity of rat IF was inconsistent with two human IMH pre parations. Rat IF was once used in the treat ment of pernicious anemia patients with success (30). Our results with human IMH, however, have not been conclusive because of the quality of the material.…”

Section: Discussioncontrasting

confidence: 47%

Attachment of the B12-Binding Principles Obtained from Lactobacillus leichmannii to Intestinal Mucosa Homogenates

Sasaki

1971

J. Vitaminol

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The B12-binding principles isolated from cellular components of Lacotobacillus leichmannii ATCC 7830 were studied for the activity to augment vitamin B12 uptake by intestinal mucosa homogenates (IMH) of the guinea pig, hog, rat, hamster, and man. It was found that the uptake of B12 by the IMH of these species was markedly augmented by these principles. The degrees of augmenta tion were much greater than that effected by the homologous intrinsic factor (IF). The IF-augmented uptake of B12 by guinea pig IMH was reduced when calcium ion was deleted from the incubation medium, while the deletion of calcium ion had no effect on the uptake augmented by the B12-binding principle. Boiling of IMH for 10min completely abolished the IF-augmented uptake of B12 but the effect of the B12-binding principle was not affected at all. These results indicate that difference exists between IF and the B12-binding principle in the mode of attachment to the adsorptive surface of the small intestine. The B12 uptake by everted sacs prepared from the distal portion of guinea pig ileum was slightly augmented by guinea pig IF but not by the B12-binding princinciple. However, the mucous materials liberated into the medium from the everted sacs during incubation took up large amounts of B12 in the presence of the B12 binding principle as well as IF.

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Section: Discussioncontrasting

confidence: 47%

Attachment of the B12-Binding Principles Obtained from Lactobacillus leichmannii to Intestinal Mucosa Homogenates

Sasaki

1971

J. Vitaminol

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“…Studies with everted sacs of small intestine from gastrectomized rats may provide useful information in this connection. Finally, the demonstration that hog intrinsic factor concentrate will enhance vitamin B12 uptake by rat small intestine further weakens the indirectly (22) and directly (17) attacked concept of species specificity of intrinsic factor as a phenomenon of major importance. Data previously reported (8) would suggest the interesting speculation that perhaps an organ specificity may exist for intrinsic factor and may be of greater significance than the species specificity.…”

Section: Resultsmentioning

confidence: 99%

Mechanism of Intrinsic Factor Action in Everted Sacs of Rat Small Intestine*

Herbert¹

1959

J. Clin. Invest.

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Three decades have passed since Castle (1) and his associates demonstrated the existence of intrinsic factor, yet knowledge of its mechanism of action is still so limited that in 1958 two workers felt justified in concluding there was no such thing as intrinsic factor (2). In prior studies (3, 4), a mechanism involved in the enhanced uptake of vitamin B12 by rat liver slices in the presence of hog intrinsic factor concentrate (HIFC) was demonstrated. This mechanism appeared to be dependent on the presence on liver slices of receptors for intrinsic factor, which in the presence of calcium would "take up" nonmetabolically either free intrinsic factor or intrinsic factor bound to vitamin B12. The present studies indicate the same mechanism may operate in the rat small intestine. A preliminary report of this work has been presented (5). MATERIALS AND METHODSThe basic protocol for each experiment was that previously used in studies with rat liver slices (4). It consisted of two successive one hour incubations of everted sacs (6) of rat small intestine at 60 C. in air; the first with 0.5 mg. HIFC 1 in the Krebs-Ringer-Tris (4) incubation medium, the second with 0.01 Ag. vitamin B,2-Coso added. Controls were run identically, except for exclusion of HIFC. Radioactivity retained in the mucosa of the everted sacs after three washings was determined in a well-type scintillation counter. Each sample was counted

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“…For example, pig intrinsic factor is active in man, though resistance may develop after prolonged use (Blackburn, Spray, Swan, Tudhope & Wilson, 1959). Rat gastric juice promoted the absorption of vitamin B,, in man (Abels, Woldring, Vegter & Nieweg, 1957) and human intrinsic factor is active in the rat (Taylor et al 1958). Intrinsic factor combines with or binds vitamin B,,, but this property is shared by other proteins which are not active as intrinsic factor (Bird & Hoevet, 1951;Abels, Nieweg & Schipperijn, 1964).…”

Section: Source Nature and Mode Of Action Of Intrinsic Factormentioning

confidence: 99%

Absorption of vitamin B₁₂from the intestine

Spray

1967

Proc. Nutr. Soc.

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Effect of Rat Intrinsic Factor on Vitamin B12 Absorption in Pernicious Anemia

Cited by 9 publications

References 2 publications

Attachment of the B12-Binding Principles Obtained from Lactobacillus leichmannii to Intestinal Mucosa Homogenates

Attachment of the B12-Binding Principles Obtained from Lactobacillus leichmannii to Intestinal Mucosa Homogenates

Mechanism of Intrinsic Factor Action in Everted Sacs of Rat Small Intestine*

Absorption of vitamin B₁₂from the intestine

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Effect of Rat Intrinsic Factor on Vitamin B12 Absorption in Pernicious Anemia

Cited by 9 publications

References 2 publications

Attachment of the B12-Binding Principles Obtained from Lactobacillus leichmannii to Intestinal Mucosa Homogenates

Attachment of the B12-Binding Principles Obtained from Lactobacillus leichmannii to Intestinal Mucosa Homogenates

Mechanism of Intrinsic Factor Action in Everted Sacs of Rat Small Intestine*

Absorption of vitamin B12from the intestine

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Product

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Absorption of vitamin B₁₂from the intestine