Effect of recombinant human platelet‐derived growth factor‐BB and bone morphogenetic protein‐2 application to demineralized dentin on early periodontal ligament cell response

Abstract

The purpose of this study is to investigate the early responses of human periodontal ligament cells attached to recombinant human platelet-derived growth factor-BB and bone morphogenetic protein-2 applied EDTA-demineralized dentin. One hundred and seventy-four root-planed flat dentin blocks were prepared from the mid-third of periodontally diseased human tooth roots. After demineralization with 24% EDTA (pH 7.02) 120 dentin blocks were treated with 0.5 and 1 microgram/ml rhPDGF-BB, 1 and 3 micrograms/ml rhBMP-2 and only MEM as control (24/group). Human periodontal ligament cells (HPLC) were seeded on these dentin surfaces and incubated. The alkaline phosphatase (ALP) activity and protein concentration of the attached cell were assessed at d 2, 4 and 7. Fifty-four dentin blocks were seeded with HPLC after application of 1 microgram/ml rhPDGF-BB, 3 micrograms/ml rhBMP-2 and MEM (18/group) and then incubated. At d 2, 4 and 7, the attached cells were stained and counted under light microscope. The results showed a significant increase of protein concentration and cell number in PDGF-BB treated groups than control (p < 0.05, p < 0.01) but not the ALP activity, and a significant increase of ALP activity was observed in BMP-2 treated groups than control (p < 0.05) but protein concentration and cell number remained almost the same over time. Thus, rhPDGF-BB and rhBMP-2 application to EDTA demineralized dentin surfaces promote the early human periodontal ligament cell responses by increasing cell proliferation and differentiation, respectively, which would ultimately enhance periodontal regeneration.

Human periodontal ligament fibroblast response to rhPDGF-BB application on periodontally diseased root surfaces-in vitro

“…Similar findings were observed in studies carried out by Belal et al (2006), Zaman et al (1999). On day 7, Group 4 also showed slight increase in number of cells (50 AE 8.9) attached.…”

“…Fibroblasts have shown maximum proliferative as well as chemotactic response to PDGF-BB homodimer (Oates et al, 1993). Exogenous addition of PDGF has shown to significantly improve cell migration, cell proliferation and the mitotic activity of PLFs, (Oates et al, 1993) but little is known about the influence of PDGF-BB on the morphology of fibroblasts in spite of the innumerable research done on rhPDGF-BB (Belal, 2006;Gamal et al, 1998;Jayakumar et al, 2011;Mumford et al, 2001;Nevins et al, 2003Nevins et al, , 2005Oates, 1993;Sant'Ana et al, 2007;Sarment et al, 2006;Zaman et al, 1999). An assessment of the influence of PDGF-BB on the morphology of PLFs can provide added information to the existing literature about the influence of rhPDGF-BB on PLFs.…”

Mesenchymal stem cells in the dental tissues: perspectives for tissue regeneration

“…The resultant improved regenerative capability could be related to increased recruitment of progenitor MSC, which subsequently differentiate to form PDL tissue. Recently, PDLSC transfected with expression vectors for platelet-derived growth factor and bone morphogenetic protein were investigated in periodontal tissue engineering models (40). These studies revealed the regeneration of normal periodontal tissues, containing organized cementum, alveolar bone and the PDL attachment apparatus.…”

Regulatory mechanisms of periodontal regeneration

“…The application of recombinant human platelet-derived growth factor and recombinant human bone morphogenetic protein-2 to EDTA demineralized dentin surfaces promotes early human periodontal ligament cell responses by increasing cell proliferation and differentiation, ultimately enhancing periodontal regeneration (Zaman et al, 1999). More recently, Zhu et al (2001) have demonstrated that Ad2/platelet-derived growth factor can effectively transduce cells derived from the periodontium and can promote biological activity equivalent to platelet-derived growth factor-AA.…”