Effect of Red Blood Cell Aging In Vivo on Their Aggregation Properties In Vitro: Measurements with Laser Tweezers

Ermolinskiy, Petr B.; Луговцов, А.Е.; Yaya, François; Lee, Kisung; Kaestner, Lars; Wagner, Christian; Priezzhev, Alexander V.

doi:10.3390/app10217581

Cited by 22 publications

(15 citation statements)

References 36 publications

(61 reference statements)

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“…Depending on the density difference between the RBCs and the reference fluid, the RBCs could potentially disappear in the reference fluid stream, owing to RBC sedimentation. To avoid RBC disappearance from the reference fluid stream, the density of the reference fluid needed to be larger than that of the RBCs (i.e., ρ RBC = 1.085–1.122 g/mL) [ 16 , 33 ]. Instead of 1× PBS ( ρ PBS = 1 g/mL), a glycerin solution (40%) ( ρ Glycerin =1.1145 g/mL) was selected as the reference fluid [ 37 ].…”

Section: Resultsmentioning

confidence: 99%

“…These alterations contribute to a reduction of mass transport and interrupt the blood flow in capillary vessels. To effectively detect changes in blood samples from a physical point of view, several rheological properties (i.e., viscosity [ 3 , 4 , 5 , 6 ], viscoelasticity [ 7 ], red blood cell (RBC) deformability [ 8 , 9 , 10 , 11 , 12 , 13 ], RBC aggregation [ 14 , 15 , 16 , 17 ], RBC sedimentation rate [ 18 , 19 ], and Hct [ 20 ]) have been measured for screening or diagnosing various diseases, including coronary heart disease, hypertension, diabetes [ 21 ], sickle cell anemia [ 22 , 23 ], and malaria. Plasma protein (i.e., fibrinogen) contributes to increasing RBC aggregation.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Blood Biophysical Properties Using Pressure Sensing with Micropump and Microfluidic Comparator

Kang

2022

Micromachines

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To identify the biophysical properties of blood samples consistently, macroscopic pumps have been used to maintain constant flow rates in a microfluidic comparator. In this study, the bulk-sized and expensive pump is replaced with a cheap and portable micropump. A specific reference fluid (i.e., glycerin solution [40%]) with a small volume of red blood cell (RBC) (i.e., 1% volume fraction) as fluid tracers is supplied into the microfluidic comparator. An averaged velocity (<Ur>) obtained with micro-particle image velocimetry is converted into the flow rate of reference fluid (Qr) (i.e., Qr = CQ × Ac × <Ur>, Ac: cross-sectional area, CQ = 1.156). Two control variables of the micropump (i.e., frequency: 400 Hz and volt: 150 au) are selected to guarantee a consistent flow rate (i.e., COV < 1%). Simultaneously, the blood sample is supplied into the microfluidic channel under specific flow patterns (i.e., constant, sinusoidal, and periodic on-off fashion). By monitoring the interface in the comparator as well as Qr, three biophysical properties (i.e., viscosity, junction pressure, and pressure-induced work) are obtained using analytical expressions derived with a discrete fluidic circuit model. According to the quantitative comparison results between the present method (i.e., micropump) and the previous method (i.e., syringe pump), the micropump provides consistent results when compared with the syringe pump. Thereafter, representative biophysical properties, including the RBC aggregation, are consistently obtained for specific blood samples prepared with dextran solutions ranging from 0 to 40 mg/mL. In conclusion, the present method could be considered as an effective method for quantifying the physical properties of blood samples, where the reference fluid is supplied with a cheap and portable micropump.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assessment of Blood Biophysical Properties Using Pressure Sensing with Micropump and Microfluidic Comparator

Kang

2022

Micromachines

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“…Subsequently, the OT is now being used in the investigation of an increasing number of biochemical and biophysical processes [23][24][25][26][27][28][29][30][31][32][33]. After decades of developments, convenient commercial OT machines and automatic manipulation systems with microfluidic chips [34][35][36][37] are becoming available and popular.…”

Section: Introductionmentioning

confidence: 99%

A novel methodology for detecting variations in RBCs surface antigens by cell-tearing using optical tweezers

Lin

Wang

Tien

et al. 2022

Preprint

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Background: The quantitative analysis of cell surface antigens has attracted increasing attention due to the antigenic variation recognition that can facilitate diagnoses. The whole-cell-based analysis minimizes sample size, suggesting an alternative approach for detecting the variation in cell surface antigens. The optical tweezers (OT) are practical for precise single-cell manipulations, and has been widely used as a convenient tool in interdisciplinary fields. However, employing this technology as a biosensor using a "tearing" operation is still rare. Method:This paper presents a novel methodology based on the “tearing” operation of optical tweezers (OT) incorporated with the “dilution method” of antibodies to detect variations in red blood cell (RBC) surface antigens. The RBCs attach to the corresponding antibody-coated cover glass. Then, the binding firmness between an RBC and the functionalized surface is assayed by optically tearing using gradually reduced laser powers incorporated with serial antibody dilutions. Results:The experiment result shows that the higher dilution (lower antibody concentration), the lower power (lower optical force) needed to tear off the RBC binding from the functionalized surface, i.e., the antibody dilution fold is inversely proportional to the laser power. With the relative-quantitative analysis, the variation in RBC surface antigens can be intuitively estimated by comparing the maximum allowable dilution folds. The estimation gives that the antigens on the B3-type RBC are 35.7% of that on the B-type RBC, which is consistent with the literature findings using conventional biological methods. Conclusions:This study proposes a new application of the optical tweezers as a biosensor using the optically cell-tearing operation to estimate the variation in RBC surface antigens. With the proposed methodology, the detection of antigenic variation was successfully implemented without complicated optical force calculations and labored biological processing. One drop of blood from the fingertip is more than enough to obtain a satisfactory detection. It suggests an novel approach for antigenic variation analyses based on the whole-cell operation.

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“…Blood behaves as a non-Newtonian fluid at lower shear rates, but as a Newtonian fluid at higher shear rates [1]. It is influenced significantly by hemorheological properties (i.e., viscoelasticity [2,3], hematocrit [4,5], deformability [6,7], and aggregation [8,9]), and vessel geometries (i.e., channel size and cell-free layer). Clinical vascular diseases are characterized by abnormal blood flow or unsteady blood flow [10,11].…”

Section: Introductionmentioning

confidence: 99%

Quantitative Monitoring of Dynamic Blood Flows Using Coflowing Laminar Streams in a Sensorless Approach

Kang¹

2021

Applied Sciences

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Determination of blood viscosity requires consistent measurement of blood flow rates, which leads to measurement errors and presents several issues when there are continuous changes in hematocrit changes. Instead of blood viscosity, a coflowing channel as a pressure sensor is adopted to quantify the dynamic flow of blood. Information on blood (i.e., hematocrit, flow rate, and viscosity) is not provided in advance. Using a discrete circuit model for the coflowing streams, the analytical expressions for four properties (i.e., pressure, shear stress, and two types of work) are then derived to quantify the flow of the test fluid. The analytical expressions are validated through numerical simulations. To demonstrate the method, the four properties are obtained using the present method by varying the flow patterns (i.e., constant flow rate or sinusoidal flow rate) as well as test fluids (i.e., glycerin solutions and blood). Thereafter, the present method is applied to quantify the dynamic flows of RBC aggregation-enhanced blood with a peristaltic pump, where any information regarding the blood is not specific. The experimental results indicate that the present method can quantify dynamic blood flow consistently, where hematocrit changes continuously over time.

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Effect of Red Blood Cell Aging In Vivo on Their Aggregation Properties In Vitro: Measurements with Laser Tweezers

Cited by 22 publications

References 36 publications

Assessment of Blood Biophysical Properties Using Pressure Sensing with Micropump and Microfluidic Comparator

Assessment of Blood Biophysical Properties Using Pressure Sensing with Micropump and Microfluidic Comparator

A novel methodology for detecting variations in RBCs surface antigens by cell-tearing using optical tweezers

Quantitative Monitoring of Dynamic Blood Flows Using Coflowing Laminar Streams in a Sensorless Approach

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