Effect of red light on optically trapped spermatozoa

Chow, Kay W.; Preece, Daryl; Berns, Michael W.

doi:10.1364/boe.8.004200

Cited by 10 publications

(11 citation statements)

References 16 publications

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“…From this point on, and given the availability of other laser systems also emitting in the NIR, there was a systematic effort to determine the less damaging spectral regions for biological optical trapping between 700 and 1100 nm [42,71,72,73], along with detailed analysis of the biological outcomes of 1060–1064 nm exposure [74,75,76,77,78,79,80,81,82,83,84,85].…”

Section: Photodamage In Optical Tweezersmentioning

confidence: 99%

“…Thus, warmer conditions can prime the plasmatic membrane for damage, either photothermal or photochemical (singlet oxygen). Recently, experiments trapping human spermatozoa with 1064 nm light and then measuring the swimming performance under additional 633 nm exposure have been published [84]. No direct negative results are reported, but the exposure time to the NIR light was limited to 20 s, on concerns that photodamage could occur to the cells.…”

Section: Photodamage In Optical Tweezersmentioning

confidence: 99%

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Optical Tweezers: Phototoxicity and Thermal Stress in Cells and Biomolecules

Blázquez‐Castro

2019

Micromachines

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For several decades optical tweezers have proven to be an invaluable tool in the study and analysis of myriad biological responses and applications. However, as with every tool, they can have undesirable or damaging effects upon the very sample they are helping to study. In this review the main negative effects of optical tweezers upon biostructures and living systems will be presented. There are three main areas on which the review will focus: linear optical excitation within the tweezers, non-linear photonic effects, and thermal load upon the sampled volume. Additional information is provided on negative mechanical effects of optical traps on biological structures. Strategies to avoid or, at least, minimize these negative effects will be introduced. Finally, all these effects, undesirable for the most, can have positive applications under the right conditions. Some hints in this direction will also be discussed.

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Section: Photodamage In Optical Tweezersmentioning

confidence: 99%

Section: Photodamage In Optical Tweezersmentioning

confidence: 99%

Optical Tweezers: Phototoxicity and Thermal Stress in Cells and Biomolecules

Blázquez‐Castro

2019

Micromachines

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“…The viscosity of the HEPES‐buffered mHTF with 5% SSS was varied between 1 and 15 cP, similar to native viscosities of the human male reproductive tract, by addition of methylcellulose (0.5–2%). A 2017 study from Chow et al . showed that exposure to red light increases the swimming force of spermatozoa.…”

Section: Measurements In Complex Mediamentioning

confidence: 99%

Strategies for Optical Trapping in Biological Samples: Aiming at Microrobotic Surgeons

Bunea

Glückstad

2019

Laser & Photonics Reviews

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Optical trapping and manipulation of objects down to the Ångstrom level has revolutionized research at the smallest scales in all natural sciences. The flexibility of optical trapping methods facilitates real‐time monitoring of the dynamics of biological processes in model systems and even in living cells. Different optical trapping and manipulation approaches allow displacement of nanostructures with subnanometer precision and force measurements with femtonewton precision. Due to inherent constraints of optical methods, most optical trapping experiments are performed in water or simple aqueous solutions. However, in recent years, there is an ever‐growing interest of shifting from simple aqueous media towards more biologically‐relevant media. Precise optical trapping and manipulation, combined with state‐of‐the‐art microfabrication, will enable the development of microrobotic “surgeons” with tremendous potential for biomedical and microengineering applications. This review introduces the basics of optical trapping and discusses its applications for biological samples, with focus on trapping in biological media and strategies for overcoming the challenges of optical manipulation in complex environments as a stepping‐stone for microrobotic “surgeons.”

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“…In this special issue, we highlight two exciting optical trapping applications [9][10] in the area of cell biology.…”

Section: Optical Trapping Applicationsmentioning

confidence: 99%

Biophotonics feature: introduction

Campagnola

Côté

Pavone³

et al. 2018

Biomed. Opt. Express

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We introduce the feature issue on the Optics in the Life Sciences Congress held on April 2-5, 2017 in San Diego, CA. The Congress consisted of 5 topical symposia: (i) Bio-optics Design and Application; (ii) Novel Techniques in Microscopy; (iii) Optical Molecular Probes, Imaging and Drug Delivery; (iv) Optical Trapping Applications; and (v) Optics and the Brain. These separate symposia also held joint sessions of common interest. The following highlights some of the topics from the Congress.

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Effect of red light on optically trapped spermatozoa

Cited by 10 publications

References 16 publications

Optical Tweezers: Phototoxicity and Thermal Stress in Cells and Biomolecules

Optical Tweezers: Phototoxicity and Thermal Stress in Cells and Biomolecules

Strategies for Optical Trapping in Biological Samples: Aiming at Microrobotic Surgeons

Biophotonics feature: introduction

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