Effect of reduced energy, protein and entire substitution of inorganic phosphorus by phytase on performance and bone mineralisation of laying hens

Lei, Qiao; Shi, Lei; Zhang, K Y; Ding, X M; Bai, Shiping; Liu, Y G

doi:10.1080/00071668.2011.562875

Cited by 19 publications

(11 citation statements)

References 36 publications

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“…As discussed by Lei et al (2011), the phytase already present in the negative controls alone could be enough to compensate for a small restriction in energy, protein and amino acids. Furthermore, this weak response could indicate that the nutritional levels of the positive control diet were above the requirement of hens for most of the evaluated variables.…”

Section: Discussionmentioning

confidence: 99%

Phytase and protease supplementation for laying hens in peak egg production

Vieira

Barbosa

Tavares

et al. 2016

SCA

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The effects of enzyme combinations in diets for commercial laying hens need further clarification. The goal of this study was to determine if the type of protease used in diets supplemented with phytase affects performance, nutrient intake, egg quality or intestinal mucosa morphometry of laying hens during peak egg production. Seven hundred and eighty hens (25-week-old Hy-Line W36 hens) were assigned to a completely randomized design composed of five treatments/diets with 12 replicates of 13 birds each. The five treatments were: 1) positive control: diet formulated according to the Hy-Line nutritional recommendations, without proteases , 2) negative control A: positive control diet reduced in energy, protein and amino acids according to protease A matrix, without protease supplementation, 3) negative control B: positive control diet reduced in energy, protein and amino acids according to protease B matrix, without protease supplementation, 4) negative control A plus protease A, 5) negative control B plus protease B. There was no effect of the treatments (P > 0.05) on egg production, egg mass or feed conversion; however, the nutritional restriction imposed by the negative controls reduced egg weight (negative control A, P=0.02), albumen height (P < 0.01) and the Haugh unit (P < 0.01). Protease supplementation reduced the calculated intake of protein and amino acids compared to the positive control; nevertheless, protease A was able to maintain egg weight, albumen height and the Haugh unit at the same levels as that obtained with the positive control hens. The intestinal mucosa responded to treatment only at the jejunum (P < 0.01), but the negative controls did not modify villus height or crypt depth compared to the positive control. However, crypt depth of protease B hens was higher than that of the positive control hens. In conclusion, when included in diets supplemented with phytase, the type of protease affects performance, nutrient intake, egg quality and intestinal mucosa morphometry of laying hens during peak egg production. Key words: Amino acid. Egg production. Egg quality. Enzyme. Nutritional restriction. ResumoOs efeitos da utilização em conjunto de enzimas exógenas para aves de postura precisam ser mais explorados na literatura. No intuito de determinar se o tipo de protease, em dietas suplementadas com fitase, interfere no desempenho, qualidade do ovo, ingestão de nutrientes e morfometria da mucosa intestinal de galinhas em pico de postura, 780 galinhas Hy-Line W36 de 25 semanas foram distribuídas em um delineamento inteiramente casualizado composto por cinco tratamentos/dietas com 12 repetições de 13 aves cada. Os cinco tratamentos foram: 1) controle positivo: dieta formulada de acordo com as recomendações nutricionais da Hy-Line, sem proteases, 2) controle negativo A: dieta controle positivo reduzida em energia, proteína e aminoácidos conforme a matriz nutricional da protease A, sem proteases, 3) controle negativo B: dieta controle positivo reduzida em energia, proteína e aminoácidos confor...

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Section: Discussionmentioning

confidence: 99%

Phytase and protease supplementation for laying hens in peak egg production

Vieira

Barbosa

Tavares

et al. 2016

SCA

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“…Shell weight (g) and shell thickness (mm) were not affected by NSP enzymes and phytase supplementation to BD (TABLE 5). [21] Reported no effect of phytase supplementation to low inorganic phosphorus, energy and protein diets on shell weight and shell thickness. Specific gravity was not affected by addition of BD with NSP enzymes and phytase individually or in combination (TABLE 5).…”

Section: Egg Quality Traitsmentioning

confidence: 99%

“…[22] Reported that the addition of 600 FTU/kg phytase improved the metabolism of nutrients in nutritionally deficient diets. [21] Reported increased tibia ash per cent on supplementation of phytase to low inorganic phosphorus, energy and protein diets. Phosphorus digestibility significantly improved on supplementing phytase to corn soybean meal based diets [17].…”

Section: Nutrient Retentionmentioning

confidence: 99%

Additive Effect of NSP Enzymes and Phytase on Performance, Egg Quality, Nutrient Retention and Gut Health of Laying Hens Fed Corn-Soybean Meal Based Low Energy Diets*

2013

IOSR-JAVS

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A trial was conducted to evaluate pure NSP enzyme combination derived from in vitro studies and commercially available phytase to corn-soybean meal based low energy diets singly and combination of both. The experiment was conducted by using completely randomized design on one hundred and fifty layer birds (40 weeks) of uniform body weight and production with five treatments, six replicates and five hens in each replicate for three laying periods with twenty eight days in each laying period. The performance was measured in terms of egg production, feed intake, weight changes, feed efficiency, egg quality, nutrient retention, and gut health. Egg production improved (P<0.05) with supplementation of phytase alone or in combination of phytase and NSP enzymes. No effect of supplementing NSP enzymes, phytase alone or in combination was observed on feed intake, FCR, egg quality traits and retentions of DM, OM and NFE. Significantly (P<0.05) higher retentions of CP, CF, EE, GE and phosphorus was observed with supplementation of NSP enzymes and similar trend was observed with both NSP and phytase to BD except for phosphorus indicating no associative effect of phytase and NSP enzymes on above nutrient retentions. Intestinal pH, viscosity and E. coli count significantly (P<0.05) reduced with supplementation of NSP enzymes and no further improvement was observed on these variables with supplementation of phytase with NSP enzymes. Gut histology revealed broad and disrupted villi with little goblet cell activity. No significant (P<0.05) effect on feed cost due to addition of phytase and/or NSP enzymes to BD was observed. The cost of feed to produce dozen eggs was comparable among SD, BD and BD supplemented with NSP enzymes and phytase.

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“…The use of phytase in animal industry is not only to enhance the utilization of phosphorous, but it also eliminates the anti-nutritive effects of phytate which ensure the bioavailability of minerals and phosphorous to promote bone strength (Lei et al, 2011). It also reduces the fecal phosphorous contents up to 50% (Konietzny and Greiner, 2004).…”

Section: Introductionmentioning

confidence: 99%

Optimization of phytase production in solid state fermentation by different fungi

Gull¹,

Hameed²,

Aslam³

et al. 2013

Afr. J. Microbiol. Res.

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The present study was conducted to explore the potential fungal isolates for maximum production of phytase by optimizing solid substrate preference and growth parameters (pH, temperature, fermentation time) of solid state fermentation (SSF). It has been inferred from the results that all the tested fungi (Aspergillus niger, Aspergillus flavus, Aspergillus versicolor, Aspergillus nidulans, Cladosporium cladosporioides, Trichoderma reesei, and Trichoderma viride) showed inducible expression of phytase. Fungal isolates showed different preference of solid substrate out of wheat bran, lentil, oat, corn and bagasse for maximum production of phytase. Lentil and bagasse were not used preferably by any tested fungi. Among the tested fungi, maximum phytase production was observed in A. flavus (80 U/g of solid substrate) using wheat bran as solid substrate at pH 6 after 7 days of fermentation period at 30°C. It was established that solid substrates with high phytate and low inorganic phosphate (Pi) contents are substrate of choice for phytase production by SSF.

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Effect of reduced energy, protein and entire substitution of inorganic phosphorus by phytase on performance and bone mineralisation of laying hens

Cited by 19 publications

References 36 publications

Phytase and protease supplementation for laying hens in peak egg production

Phytase and protease supplementation for laying hens in peak egg production

Additive Effect of NSP Enzymes and Phytase on Performance, Egg Quality, Nutrient Retention and Gut Health of Laying Hens Fed Corn-Soybean Meal Based Low Energy Diets*

Optimization of phytase production in solid state fermentation by different fungi

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