Effect of Removable Dentures on Colonization of Aerobic Bacteria in the Oral Cavity and Antibiotic Pattern of the Common Isolated Bacteria

Abstract

Background and aims: Wearing a removable dental prosthesis causes a change in the micro flora of the mouth. The aim of this research was to verify the composition of aerobic bacterial in the oral cavity of patients with removable dentures and with normal teeth (without dentures), and antibiotics pattern for common isolates. Methods: Bacteriological investigations were performed in 122 individuals (61 removable dentures: 61 normal teeth) attending dental clinics of Faculty of dentistry, Sana’a University, Yemen and private dental clinics. The culturing and antibiotic sensitivity were conducted in the Microbiology Department of the National Center of Public Health Laboratories (NCPHL) Sana'a, Yemen. Tongue dorsa and palate mucosa swabs were collected from normal teeth and denture groups, and as well swabs from mucosal part of denture surfaces in prosthetic patients. Cultivation in microaerophilic (5% CO2) and oxygenic conditions were performed on solid selective and non-selective media in addition to media enriched with 5% blood. Results: Regarding the prosthetic patients, the rate of bacterial isolates from the palate, back, tongue and dental plaque smears was higher potential pathogenic bacteria as S. aureus and Enterobacteriaceae spp in denture wearers, as E. coli (6.6% in dentures vs. 1.6% in the absence of dentures), Klebsiella pneumoniae (11.4% in dentures versus 1.6% in the normal teeth) and Pseudomonas aeruginosa (13.1% versus 0.0% ).While in viridians Streptococcus including Strept .mutans, there was a lower colonization rate in denture patients (18% in palate verses, 73.8% in individuals without dentures). Conclusion: The study demonstrated an elevated rate of bacterial isolates from palate, back, tongue and plaque swabs in denture patients of pathogenic bacteria such as S. aureus, E. coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa; while in Streptococcus viridians including S. mutans, there was a lower colonization rate in denture patients verses a very high rate in individuals without dentures. Peer Review History: Received: 7 November 2022; Revised: 13 December; Accepted: 8 January 2023, Available online: 15 January 2023 Academic Editor: Ahmad Najib, Universitas Muslim Indonesia, Indonesia, ahmad.najib@umi.ac.id Received file: Reviewer's Comments: Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 7.0/10 Reviewers: Dr. Alfonso Alexander Aguileral, University of Veracruz, Mexico, aalexander_2000@yahoo.com Dr. A.A. Mgbahurike, University of Port Harcourt, Nigeria, amaka_mgbahurike@yahoo.com Similar Articles: ETIOLOGY AND RISK FACTORS OF STOMATITIS AMONG YEMENI DENTURE WEARERS PREVALENCE AND ASSOCIATED FACTORS OF ORAL NON-CANDIDA ALBICANS CANDIDA CARRIAGE IN DENTURE WEARERS IN SANA'A CITY- YEMEN THE EFFECT OF NANOSILVER AND CHLORHEXIDINE MOUTHWASH ON ANAEROBIC PERIODONTAL PATHOGENS COUNTS

The Effect of Dental Implants on Secretory Iga Level in Saliva

“…After positioning the implant fixture to maximize osseointegration, a dental prosthesis is affixed. Before the dental prosthetic (a tooth, bridge, or denture) is affixed to the implant or an abutment is positioned to support a dental prosthetic/crown, osseointegration must take a varied period of healing time [16,17].…”

“…It was reported that during the healing period needed for osseointegration, there was a variable degree of change in the oral conditions, such as changes in the colonization of oral microorganisms and oral s-IgA level, before either the dental prosthetic (a tooth, bridge, or denture) is attached to the implant or an abutment is placed to hold a dental prosthetic/crown [16,17]. Implant success or failure is largely determined by the thickness and condition of the bone and gingival tissues surrounding the implant, as well as by factors such as serum immunoglobulin A (sIgA), gingival sulcus cytokines, oral microbial balance, patient health, and medications that alter the likelihood of osseointegration [18][19][20].…”

The Prevalence and Associated Factors of Staphylococcus Aureus Colonization in the Oral Cavity of Adults

“…It also caused oral infections. Acute dentoalveolar abscess, dry mouth, denture wearers, and angular chelitis [29][30][31][32][33] , among other oro-facial illnesses, have also been linked to S. aureus in the oral cavity has been linked to systemic infections and potentially fatal conditions include aspiration pneumonia, rheumatoid arthritis, and infective endocarditis 23,34-36 . When there are more colonization in oral cavity, there is a higher chance of infections, which can change oral and overall health issues.…”