Effect of repeated eCG treatments and ovum pick-up on ovarian response and oocyte recovery during early pregnancy in suckling beef cows

Aller, Juan F.; Mucci, N.; Kaiser, G; Callejas, S.; Alberio, R. H.

doi:10.1016/j.anireprosci.2012.06.001

Cited by 16 publications

(12 citation statements)

References 35 publications

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“…In the current study, pregnant cows were independently associated with a decreasing number of oocytes aspirated per cow after adjusting for the random effect of the day on which ovaries were harvested (Table 3). These results correlate with findings of a number of studies where significantly reduced follicle and oocyte numbers were described in pregnant cows [35][36][37].…”

Section: Several Studies Reported Higher Recovery Rates Of Viable Oocsupporting

confidence: 90%

Serum albumin concentration of donor cows as an indicator of developmental competence of oocytes

et al. 2019

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Highlights• Serum albumin level of donor cows independently and significantly predicts developmental competence of oocytes.• Adjusting the levels of protein supplementation in in vitro culture protocols based on donor serum albumin may be warranted.• Albumin levels in follicular fluid are not correlated, indicating regulatory mechanism that determines follicular fluid albumin level. AbstractAdequate nutrition is required for maintenance of normal reproduction in cattle.Albumin, the best marker and fundamental part of nutrition, most abundant plasma protein and major component of fetal bovine serum, is the best predictor of malnourishment in South African cattle. The aim of this study was to determine if serum albumin concentrations of donor cows predict the developmental competence of oocytes, and if additional protein supplementation of the in vitro culture media improves embryo outcomes in oocytes from cows with inadequate serum albumin concentrations. Oocytes (n = 1024) were recovered from donors with inadequate (≤35.9 g/L), or adequate serum albumin concentrations (≥36.0 g/L).Four hundred and sixty oocytes originated from cows with inadequate serum albumin and 564 from cows with adequate serum albumin. Oocytes of these cohorts were randomly allocated to a control and supplemented fetal bovine serum in vitro embryo culture protocol. Multiple linear, logistic and Poisson regression analyses were performed to estimate the effects of

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Section: Several Studies Reported Higher Recovery Rates Of Viable Oocsupporting

confidence: 90%

Serum albumin concentration of donor cows as an indicator of developmental competence of oocytes

et al. 2019

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“…cows that had not produced a viable embryo for 1 or more years in a conventional MOET programme) of different breeds in an earlier trial (Looney et al 1994). In contrast, these parameters were similar to those recorded in suckling beef cows (Aller et al 2012). It is known that oocytes recovery depends on the number of antral follicles with diameter > 2 mm, and that follicular population is affected by genetics, breeding system, nutrition, body condition score, and stage of lactation (Lucy et al 1992;Pushpakumara et al 2003;Kouamo et al 2015).…”

Section: Discussionmentioning

confidence: 75%

“…It has been speculated that the larger volume, associated with increased viscosity and higher intra-follicular pressure of larger follicles may in part interfere with oocyte collection during the insertion and rotation of the needle inside the follicle. In contrast, in suckling beef cows recovery rate was not affected by the treatment with a higher dose of eCG, thus the increased number of follicles resulted in increased number of oocytes and blastocysts (Aller et al 2012). It can be concluded that the treatment with eCG, although increasing the number of medium follicles and oocyte competence, was not effective in maximising either the number of oocytes for IVEP or the overall number of embryos produced.…”

Section: Discussionmentioning

confidence: 80%

Efficacy of repeated ovum pick-up in Podolic cattle for preservation strategies: a pilot study

Presicce

Neglia

Salzano

et al. 2019

Italian Journal of Animal Science

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The study evaluated the effects of eCG treatment prior to ovum pick up (OPU) on follicular population, oocyte and embryo yields in summer and autumn in Podolic cattle. The effects of repeated OPU on cattle wellbeing was also documented. Twenty-six animals were used, and split into two groups, treatment (OPU; n ¼ 18) and control (CG; n ¼ 8). The OPU cattle were subsequently split into two subgroups (n ¼ 9) and underwent repeated OPU, without and with eCG priming, for a total of 8 sessions (4 sessions/season). Follicular population, oocyte and embryo yields were recorded in those subgroups. CG was handled in the same manner of OPU, except for epidural anaesthesia and follicular aspiration. Biochemical profile, serum protein electrophoresis and haptoglobin levels were analysed in OPU and CG. Hormonal priming increased the number of medium follicles (1.7 ± 0.2 vs 0.9 ± 0.2, p < .05), while it decreased the recovery rate and number of cumulus-enclosed oocytes (COCs) (recovery rate: 38.3 ± 3.5 vs 60.5 ± 4.0%; COCs: 2.3 ± 0.3 vs 3.4 ± 0.4, respectively; p < .01). However, priming increased cleavage (72.9 ± 5.7 vs 49.4 ± 5.4; p < .05) and blastocyst (41.1 ± 5.7 vs 23.0 ± 4.2; p ¼ .054) rates. With regard to season's effect, a higher number of COCs was recorded in autumn than in summer (3.1 ± 0.4 vs 2.6 ± 0.3; p < .05) without affecting though the number of embryos produced (0.9 on average). Since haematological parameters did not vary between OPU and CG, our preliminary data suggest that repeated OPU may be used as a conservation strategy in Podolic cattle without affecting wellbeing. HIGHLIGHTS Podolic cattle is an endangered Italian breed, reared in semi-extensive/extensive systems Ovum pickup (OPU) can be carried out in this breed without impairing animal health and welfare The eCG treatment before OPU (priming) did not improve the number of embryos produced per donor.

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“…However, OTU can also be applied in pregnant cows, in which collection of oocytes by OPU is possible in the first three months of pregnancy (72)(73)(74)(75)(76). Treatment and sampling of ovaries can be carried out with surgical techniques (77), but these invasive procedures are impractical for serial treatment and sampling on a regular basis.…”

Section: Relevance Of Using Otu As Research Tool In Bovine Reproductionmentioning

confidence: 99%

“…OTU devices can be used safely in calves (52, 84) and the general consensus is that neither well-being nor health of bovine donors subjected to OPU is significantly affected (85)(86)(87). Although application of OTU for the aspiration of ovarian follicles can induce changes in reproductive hormone profiles (88)(89)(90)(91), follicular growth (92), and alter the length of the estrous cycle (93), the reproductive performance after termination of follicular aspirations is usually unaffected (3,76,94,95). Nevertheless, there is evidence indicating the formation of ''residual follicles'' (i.e., follicles that refilled with fluid after follicular aspiration) following OPU, which could result in the formation of follicular cysts or impaired follicular growth (96).…”

Section: Relevance Of Using Otu As Research Tool In Bovine Reproductionmentioning

confidence: 99%

Biomedical Applications of Ovarian Transvaginal Ultrasonography in Cattle

Velazquez

Kues

Niemann

2014

Animal Biotechnology

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Ovarian transvaginal ultrasonography (OTU) has been used world-wide for commercial ovum pick-up programs for in vitro embryo production in elite herds, providing an excellent model for the elucidation of factors controlling bovine oocyte developmental competence. Noninvasive sampling and treatment of ovarian structures is easily accomplished with bovine OTU techniques providing a promising system for in vivo delivery of transgenes directly into the ovary. The current review summarizes existing bovine OTU models and provides prospective applications of bovine OTU to undertake research in reproductive topics of biomedical relevance, with special emphasis on the development of in vivo gene transfer strategies.

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Effect of repeated eCG treatments and ovum pick-up on ovarian response and oocyte recovery during early pregnancy in suckling beef cows

Cited by 16 publications

References 35 publications

Serum albumin concentration of donor cows as an indicator of developmental competence of oocytes

Serum albumin concentration of donor cows as an indicator of developmental competence of oocytes

Efficacy of repeated ovum pick-up in Podolic cattle for preservation strategies: a pilot study

Biomedical Applications of Ovarian Transvaginal Ultrasonography in Cattle

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