Effect of repeated passaging and cell density on proliferation and differentiation potential of cord blood unrestricted somatic stem cells

Demerdash, Zeinab; El-Baz, Hanan; Maher, Kesmat; Hassan, S. S.; Salah, Faten; Hassan, Marwa; Elzallat, Mohamed; El-Shafei, M. E.; Taha, Tamer

doi:10.1016/j.nhtm.2014.11.043

Cited by 5 publications

(4 citation statements)

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“…This contradicts findings in other cell types. For example, ESC core factor expression in cord blood stem cells is highest at HD ( Demerdash et al., 2015 ), but LD is preferred for core factor expression in mesenchymal stem cells ( Kim et al., 2017 ). Thus, the sensitivity of genes like Oct4 and Nanog to seeding density might be highly context dependent.…”

Section: Discussionmentioning

confidence: 99%

β-catenin links cell seeding density to global gene expression during mouse embryonic stem cell differentiation

et al. 2022

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Summary Although cell density is known to affect numerous biological processes including gene expression and cell fate specification, mechanistic understanding of what factors link cell density to global gene regulation is lacking. Here, we reveal that the expression of thousands of genes in mouse embryonic stem cells (mESCs) is affected by cell seeding density and that low cell density enhances the efficiency of differentiation. Mechanistically, β-catenin is localized primarily to adherens junctions during both self-renewal and differentiation at high density. However, when mESCs differentiate at low density, β-catenin translocates to the nucleus and associates with Tcf7l1, inducing co-occupied lineage markers. Meanwhile, Esrrb sustains the expression of pluripotency-associated genes while repressing lineage markers at high density, and its association with DNA decreases at low density. Our results provide new insights into the previously neglected but pervasive phenomenon of density-dependent gene regulation.

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Section: Discussionmentioning

confidence: 99%

β-catenin links cell seeding density to global gene expression during mouse embryonic stem cell differentiation

et al. 2022

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“…Demerdash et al. () reported that MSCs exhibit high expression of pluripotency genes in early passages, and this expression decreases gradually with increasing passages.…”

Section: Discussionmentioning

confidence: 99%

Transplantation of amniotic membrane‐derived multipotent cells ameliorates and delays the progression of chronic kidney disease in cats

Vidane

Pinheiro

Casals

et al. 2016

Reprod Domestic Animals

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“…In a subsequent study, Hutton et al (24) replaced the FBS with a serum substitute and added basic fibroblast growth factor. Demerdash et al (25) indicated that the number of expansion times of the feeder culture system were limited and that the growth ability of UCBSCs was reduced following differentiation for a certain number of generations. Furthermore, the authors of that study demonstrated that feeder layer cells weakened the effect of exogenous factors on UCBSCs and the animal/human-derived feeder layer was unable to achieve its clinical effect due to contamination with heterogenic/allogeneic antigens (23,(26)(27)(28)(29).…”

Section: Differentiation Of Ucbscsmentioning

confidence: 99%

DNA barcode to trace the development and differentiation of cord blood stem cells (Review)

et al. 2021

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Umbilical cord blood transplantation was first reported in 1980. Since then, additional research has indicated that umbilical cord blood stem cells (UCBSCs) have various advantages, such as multi-lineage differentiation potential and potent renewal activity, which may be induced to promote their differentiation into a variety of seed cells for tissue engineering and the treatment of clinical and metabolic diseases. Recent studies suggested that UCBSCs are able to differentiate into nerve cells, chondrocytes, hepatocyte-like cells, fat cells and osteoblasts. The culture of UCBSCs has developed from feeder-layer to feeder-free culture systems.The classical techniques of cell labeling and tracing by gene transfection and fluorescent dye and nucleic acid analogs have evolved to DNA barcode technology mediated by transposon/retrovirus, cyclization recombination-recombinase and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 strategies. DNA barcoding for cell development tracing has advanced to include single cells and single nucleic acid mutations. In the present study, the latest research findings on the development and differentiation, culture techniques and labeling and tracing of UCBSCs are reviewed. The present study may increase the current understanding of UCBSC biology and its clinical applications.

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Effect of repeated passaging and cell density on proliferation and differentiation potential of cord blood unrestricted somatic stem cells

Cited by 5 publications

References 0 publications

β-catenin links cell seeding density to global gene expression during mouse embryonic stem cell differentiation

β-catenin links cell seeding density to global gene expression during mouse embryonic stem cell differentiation

Transplantation of amniotic membrane‐derived multipotent cells ameliorates and delays the progression of chronic kidney disease in cats

DNA barcode to trace the development and differentiation of cord blood stem cells (Review)

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