Effect of reserpine on cell proliferation in the developing rat brain: A biochemical study

Patel, Ambrish J.; Béndek, G.; Balázs, R.; Lewis, Paul D.

doi:10.1016/0006-8993(77)90008-7

Cited by 51 publications

(6 citation statements)

References 28 publications

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“…The transitory presence of fibers containing a large amount of DBH in this intense proliferation area could suggest a relationship between NA and cell division. However, this relationship al ready taken in account by others [13,17,18] has not been clearly demonstrated yet.…”

Section: Discussionmentioning

confidence: 90%

Distribution of Dopamine-Beta-Hydroxylase-Like Immunoreactive Fibers in the Rat Cerebellar Cortex During Ontogeny

1982

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Postnatal ontogeny of dopamine-β-hydroxylase (DBH)-like immunoreactive fibers in the rat cerebellar cortex has been studied by immunofluorescence on sagittal sections of the vermis. Except in the case of unusual thick highly fluorescent fibers noticed in the external granular layer during the first 2 weeks after birth, the arrival of DBH-containing fibers through the cerebellar cortex follows an inside-out gradient: at birth sparse fluorescent axons are observed in the album of the cerebellum. They invade the internal granular layer at postnatal day 2 (P2) and reach the Purkinje cell layer at P5. From P5 to P15, the density of fibers increases in this layer, which becomes the most innervated layer of the cerebellar cortex, as in the adult. When the external grains migrate, DBH-containing fibers extend progressively from depth to surface in the molecular layer and show an adult pattern at P30. The relationship between our results and other morphological and biochemical studies is discussed.

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Section: Discussionmentioning

confidence: 90%

Distribution of Dopamine-Beta-Hydroxylase-Like Immunoreactive Fibers in the Rat Cerebellar Cortex During Ontogeny

1982

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“…The cerebellar thymidine kinase activ ity is 30 times higher than that of the fore brain [25]. While in the forebrain the most important proliferative area, the ventricular subependymal layer produces exclusively glia cells, in the external granular layer of cere bellum only small neurons are formed and a lesser amount of glia is produced by the internal granular layer [20].…”

Section: Discussionmentioning

confidence: 94%

“…In contrast with our previous studies using drugs to deplete catecholamines such as reserpine, 6-OHDA and tetrabenazine [24][25][26], which are CNS depressants or used for chemi cal sympathectomy, in this study we used amphetamine, an antidepressant with pro foundly different chemical structure and be havioral effect. Since amphetamine has been reported to disturb development [15] and pro-tein synthesis in the brain at high doses [22,35], and is also known to deplete norepineph rine stores in brain [16], it seemed to be of interest to investigate whether any changes of [3H]-thymidine incorporation could be de tected in the developing rat brain after amphe tamine treatment.…”

Section: Introductionmentioning

confidence: 99%

Effect of Amphetamine on the Metabolism and Incorporation of [<sup>3</sup>H]-Thymidine into DNA of Developing Rat Brain

Béndek¹,

Hahn²

1981

Dev Neurosci

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High doses of dl-amphetamine (15–40 mg/kg in free base, s.c.) inhibited the cell proliferation in the forebrain of 10-day-old rats 2 h after treatment, in terms of the incorporation of [³H]-thymidine into DNA. The relative specific radioactivity of DNA was decreased to 70% compared to controls. The norepinephrine level of the forebrain was reduced to 30–50% of the control value. The observed elevation of plasma corticosterone level did not appear to mediate the effect on cell proliferation. Kinetic studies indicated that the entry of precursor from the blood to the brain tissue was enhanced, but the higher tissue concentration of precursor was not utilized in the DNA synthesis with the same speed. The phosphorylation of radioactive thymidine to thymidine phosphates was only slightly inhibited, and the suppression of DNA synthesis was evident even after the correction made on the basis of concentration of radioactive thymidine phosphates available for synthesis. DNA synthesis in the cerebellum was not suppressed by amphetamine treatment. The possible connection between the change in norepinephrine pools and the proliferative activity is considered.

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“…Homogenates were centrifuged at 35,000 x g for 30 min at O°C and the resulting supernatants were removed and frozen. On the day of the assay, supernatants were thawed and duplicate aliquots analyzed for thymidine kinase activity by the radioisotopic method of Patel et al (1977) with minor modifications. Reagent blanks with homogenization buffer used in place of tissue extracts were analyzed in parallel in each assay and results corrected by subtracting blank values from all sample values in that assay.…”

Section: Methodsmentioning

confidence: 99%

Comparison of Postnatal Brain Development in Meadow Voles (Microtus pennsylvanicus) and Pine Voles (Microtus pinetorum)

Gutierrez

Meyer

Novak

1989

Journal of Mammalogy

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Previous studies on behavioral maturation and responses to neonatal administration of hydrocortisone in meadow voles (Microtus pennsylvanicus) and pine voles (M. pinetorum) raised the possibility that temporal development of the brain might differ in these two closely related species. This hypothesis was tested by measuring changes in brain mass (whole brain, cerebrum, and cerebellum) and thymidine kinase activity (an index of mitotic activity) in the cerebrum and cerebellum of both species from day 2 to day 35 postpartum. Brain mass and enzyme activity indicated an accelerated rate of brain development in meadow voles compared to pine voles, particularly during the first 1-2 weeks of postnatal life. This conclusion was further supported by results of brain-body mass allometry performed on the same subjects. Finally, encephalization quotients calculated for adult (65-day-old) animals revealed that meadow voles but not pine voles possess smaller brains relative to their body masses than expected.Meadow voles (Microtus pennsylvanicus) occupy a grassland habitat, appear to be promiscuous (Madison, 1980), and mainly females care for young (Gruder-Adams and Getz, 1985;McGuire and Novak, 1984;Oliveras and Novak, 1986). In contrast, pine voles (M. pinetorum) are found in a deciduous, orchard habitat, display a monogamous mating system (FitzGerald and Madison, 1983), and both sexes show a substantial amount of parental care (McGuire and Novak, 1984;Oliveras and Novak, 1986). Recent findings in our laboratory suggest that these two species may differ not only in habitat selection and social organization but also with respect to their rate of postnatal behavioral maturation. The present investigation of brain development in meadow and pine voles was conducted to determine whether a neurological basis for such differing behavioral development could be identified.Using behavioral indices such as last day of observed nipple attachment, McGuire and Novak (1984) found that meadow voles usually are weaned considerably earlier (approximately day 13) than pine voles (approximately day 21). Although several explanations can be offered for this result, one possibility is a more rapid maturation of adult feeding capabilities and maternal independence on the part of meadow voles compared to pine voles. Prohazka et al. (1986) compared these two species in terms of the development of swimming, a complex motor behavior that can be quantified along dimensions such as the animal's head and nose position in the water and the progressive inhibition of front paw movements as the animal matures. On both measures, meadow voles approached their adult Swimming pattern more rapidly than pine voles. Finally, in the same study Prohazka et al. (1986) investigated developmental effects of a single injection of hydrocortisone given on day 2 postpartum. Previous experiments using albino rats (Rattus norvegicus) generally revealed retardative influences of hydrocortisone on brain and body growth, and on behavioral maturation (Balazs and Richter, 1973; Co...

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Effect of reserpine on cell proliferation in the developing rat brain: A biochemical study

Cited by 51 publications

References 28 publications

Distribution of Dopamine-Beta-Hydroxylase-Like Immunoreactive Fibers in the Rat Cerebellar Cortex During Ontogeny

Distribution of Dopamine-Beta-Hydroxylase-Like Immunoreactive Fibers in the Rat Cerebellar Cortex During Ontogeny

Effect of Amphetamine on the Metabolism and Incorporation of [<sup>3</sup>H]-Thymidine into DNA of Developing Rat Brain

Comparison of Postnatal Brain Development in Meadow Voles (Microtus pennsylvanicus) and Pine Voles (Microtus pinetorum)

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