Effect of residual sericin on the structural characteristics and properties of regenerated silk films

Lee, Ji Hye; Song, Dae Woong; Park, Young Hwan; Um, In Chul

doi:10.1016/j.ijbiomac.2016.04.073

Cited by 39 publications

(29 citation statements)

References 20 publications

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“…On the one hand, it could be due to the presence of SS in the SFN coming from the remaining SS in the SF-D4 fibers. On the other hand, if almost all the SS had been removed during the overall process and washing steps, the SS present in the ionic liquid solution might have interfered in the molecular arrangement during SF recrystallization in the nanoparticles , leading to a decrease in the total βsheet content as previously described by Lee et al [36].…”

Section: Sf Secondary Structure Analysismentioning

confidence: 72%

“…Previous studies have found that different degumming processes affect SF yarns properties [15,23,24,32,33] and also concern the production of SF-based engineered materials like films [34][35][36], 3D scaffolds [28] or electro-spun mats [27,37]. However, to the best of our knowledge, no study on how the degumming process affects SFN formation, their structural characteristics and the changes in their size, Z-potential and surface charge density have been carried out.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Revealing the Influence of the Degumming Process in the Properties of Silk Fibroin Nanoparticles

Carissimi¹,

Lozano-Pérez²,

Montalbán³

et al. 2019

Preprint

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In recent years, numerous research studies have shown the excellent characteristics of silk fibroin nanoparticles as a vehicle for drugs delivery and it is foreseeable that their production could reach industrial scale in the coming years. For this reason, it is essential to know all the parameters that affect the formation of nanoparticles in order to standardize the process. Several studies have stated that the process used for sericin removal (degumming) from silk cocoons has a strong impact in the silk fibroin integrity and their mechanical properties after processing it into biomaterials. In this work, silk cocoons were degummed following four standard methods: autoclaving, short alkaline (Na2CO3) boiling, long alkaline (Na2CO3) boiling and ultrasounds. The resultant silk fibroin fibers were dissolved in the ionic liquid 1-ethyl-3-methylimidazolium acetate and used for nanoparticle synthesis by rapid desolvation in polar organic solvents. The relative efficiencies of the degumming processes and the integrity of the resulting fibroin fibers obtained were analyzed by weight loss, optical microscopy, thermogravimetric analysis, infrared spectroscopy and SDS-PAGE. Particle sizes and morphology were analyzed by Dynamic Light Scattering and Field Emission Scanning Electronic Microscopy. The results showed that the different treatments had a remarkable impact on the integrity of the silk fibroin chains, as confirmed by gel electrophoresis which can be correlated with particle mean size and size distribution changes. The study confirm that all the parameters of the process must be controlled in order to reach an optimum reproducibility of the nanoparticle production.

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Section: Sf Secondary Structure Analysismentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Revealing the Influence of the Degumming Process in the Properties of Silk Fibroin Nanoparticles

Carissimi¹,

Lozano-Pérez²,

Montalbán³

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…If waste sericin is recovered and recycled, it will not only reduce environmental pollution but can be used in different fields such as pharmaceutical, cosmetics, food industry, biomaterial manufacturing etc. [15][16][17][18][19][20][21] Due to high concentration of hydrophilic groups it shows excellent water retention property and these groups have strong polar side groups which imparts property of electrostatic bonding with drug molecules. 22 This character is used to prepare and stabilize nanocrystals of poorly soluble drugs which shows enhanced dissolution rates and bioavailability.…”

Section: Introductionmentioning

confidence: 99%

Hydrochlorothiazide Nanocrystals Stabilization by Silk Sericin

Giri¹,

Jadhav²,

Patil³

et al. 2019

IJPER

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Aim: The aim of present study deals with preparation and stabilization of Hydrochlorothiazide nanocrystals using silk sericin. Materials and Methods: Nanocrystals of BCS class II drugs were primed by antisolvent precipitation method in ratio of HCTZ: Sericin (1:0.5, 1:1, 1:1.5, 1:2). Further Nanocrystals were investigated by particle size, zeta potential, drug content, ATR-FTIR, XRD, DSC, SEM, in vitro dissolution study and stability study. Zeta potential (1:0.5) shows higher value-18.8 than other batches which means optimized P1 batch have more stability than other batches. Low loss of drug content in lyophilized P1 batch resulted in excellent revival of nanocrystals. In FTIR, principle peaks of pure drug HCTZ were retained in (1:0.5) P1 batch which are almost identical. Further XRD proved that HCTZ existed in crystalline state in the HCTZ nanocrystals. DSC of HCTZ and HCTZ nanocrystals peaks were found to be nearly indistinguishable. Nanocrystals were found to be sharp needle in shape. Results: Nanocrystals of P1 batch displayed enhanced dissolution performance than other batches as its % drug dissolution was found to be 89.39. % crystallinity of nanocrystals was somewhat increased but not any noteworthy difference after 3 months storage as % crystainity of P1 batch after storage for 3 months was found to be 44.01 to 45.07 hence it was increased to some extent, but difference was not noteworthy. Conclusion: Result verified that, sericin improves solubility and stability of nanocrystals. Thus, sericin play important role in stabilization of nanocrystals of poorly soluble drugs.

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“…Silk has been considered an excellent textile material for a long time because it has good luster and feel (Lee et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Effect of treatment temperature on mechanical properties of silk textiles made with silk/polyurethane core-spun yarn

Bae¹,

Um²

2016

International Journal of Industrial Entomology

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Silk has been used extensively in textile applications because of its good luster and feel. However, the low elongation and elastic recovery of silk has limited its use in a wider variety of textile applications. In this study, silk textile samples were made with a highly twisted silk/polyurethane core-spun yarn. They were immersed in water and dried at different temperatures, and the effect of treatment temperature on the mechanical properties of the silk textile was examined. It was found that the water temperature strongly affected the morphology and mechanical properties of the silk textile, whereas the drying temperature did not. As the water temperature was increased, the weft silk yarn became tangled and the interval between warp yarns decreased, resulting in shrinkage of the silk textile. When the silk textile was immersed in water at high temperature (i.e., 100°C), the elongation of the textile increased eight-fold as compared to an untreated silk textile. The maximum elastic recovery ratio of the silk textile was 96.7%.

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Effect of residual sericin on the structural characteristics and properties of regenerated silk films

Cited by 39 publications

References 20 publications

Revealing the Influence of the Degumming Process in the Properties of Silk Fibroin Nanoparticles

Revealing the Influence of the Degumming Process in the Properties of Silk Fibroin Nanoparticles

Hydrochlorothiazide Nanocrystals Stabilization by Silk Sericin

Effect of treatment temperature on mechanical properties of silk textiles made with silk/polyurethane core-spun yarn

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