Effect of resiniferatoxin on glutamatergic spontaneous excitatory synaptic transmission in substantia gelatinosa neurons of the adult rat spinal cord

Jiang, Changyu; Fujita, Teizo; Yue, Haosong; Piao, Lianhua; Liu, T.; Nakatsuka, Terumasa; Kumamoto, Eiichi

doi:10.1016/j.neuroscience.2009.09.033

Cited by 31 publications

(36 citation statements)

References 57 publications

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“…In spinal cord slices from rats injected with Freund's complete adjuvant, the TRPV1 antagonist, SB-366791, produced a decrease in the frequency of excitatory post-synaptic currents (EPSCs) (Lappin et al, 2006). TRPV1-mediated increases in EPSC frequency have been observed in the substantia gelatinosa, PAG, medial preoptic nucleus, substantia nigra and locus coeruleus (Jiang et al, 2009;Karlsson et al, 2005;Marinelli et al, 2002;Xing and Li, 2007 Regulation of bloodbrain barrier permeability Human (Golech et al, 2004) 1.5. Role of TRPV1 in pain 1.…”

Section: Physiologymentioning

confidence: 99%

Genotype-dependent responsivity to inflammatory pain: A role for TRPV1 in the periaqueductal grey

Madasu

Okine

Olango

et al. 2016

Pharmacological Research

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Section: Physiologymentioning

confidence: 99%

Genotype-dependent responsivity to inflammatory pain: A role for TRPV1 in the periaqueductal grey

Madasu

Okine

Olango

et al. 2016

Pharmacological Research

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“…Slice preparations from the adult rat spinal cord were prepared as described elsewhere [4,10,13]. Briefly, male SpragueeDawley rats (6e8 weeks old) were anesthetized with urethane (1.2 g/kg, i.p.…”

Section: Methodsmentioning

confidence: 99%

Spontaneous l-glutamate release enhancement in rat substantia gelatinosa neurons by (−)-carvone and (+)-carvone which activate different types of TRP channel

Kang

Jiang

Fujita

et al. 2015

Biochemical and Biophysical Research Communications

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“…Spinal cord slices from adult rats were prepared as described previously (Fujita and Kumamoto 2006;Jiang et al 2009;Liu et al 2004Liu et al , 2008. In brief, male adult Sprague-Dawley rats (6 -8 wk old) were anesthetized with urethane (1.2 g/kg, ip), and then a laminectomy was performed to extract a lumbosacral spinal cord enlargement (L1-S3).…”

Section: Methodsmentioning

confidence: 99%

“…The SG was identified as a translucent band under a binocular microscope with light transmitted from below (Fujita and Kumamoto 2006;Jiang et al 2009;Liu et al 2004Liu et al , 2008. Blind whole-cell voltage-clamp recordings of spontaneous inhibitory postsynaptic currents (sIPSCs) from the SG neurons were made at a holding potential (V H ) of 0 mV, where glutamatergic spontaneous excitatory postsynaptic currents were invisible because the reversal potential for non-N-methyl-D-aspartate (NMDA) receptor channels involved in this production was close to 0 mV (Iyadomi et al 2000;Kohno et al 1999;Liu et al 2008;Luo et al 2002;Yang et al 2004).…”

Section: Methodsmentioning

confidence: 99%

“…PGE 2 inhibits glycinergic inhibitory transmission in rat superficial dorsal horn neurons (Ahmadi et al 2002;for review, see Zeilhofer 2005). Hwang et al (2000) have demonstrated that the metabolites of LOX directly activate the transient receptor potential type-1 channel, which plays a role in regulating nociceptive transmission in the SG (Jiang et al 2009; for review, see Szallasi 2002).…”

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confidence: 99%

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Acetylcholine and norepinephrine mediate GABAergic but not glycinergic transmission enhancement by melittin in adult rat substantia gelatinosa neurons

Liu

Fujita

Kumamoto

2011

Journal of Neurophysiology

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Liu T, Fujita T, Kumamoto E. Acetylcholine and norepinephrine mediate GABAergic but not glycinergic transmission enhancement by melittin in adult rat substantia gelatinosa neurons. J Neurophysiol 106: 233-246, 2011. First published April 27, 2011 doi:10.1152/jn.00838.2010.-GABAergic and glycinergic inhibitory synaptic transmissions in substantia gelatinosa (SG; lamina II of Rexed) neurons of the spinal dorsal horn play an important role in regulating nociceptive transmission from the periphery. It has not yet been well known whether each of the inhibitory transmissions plays a distinct role in the regulation. We report an involvement of neurotransmitters in GABAergic but not glycinergic transmission enhancement produced by the PLA 2 activator melittin, where the wholecell patch-clamp technique is applied to the SG neurons of adult rat spinal cord slices. Glycinergic but not GABAergic spontaneous inhibitory postsynaptic current (sIPSC) was increased in frequency and amplitude by melittin in the presence of nicotinic, muscarinic acetylcholine, and ␣ 1 -adrenergic receptor antagonists (mecamylamine, atropine, and WB-4101, respectively). GABAergic transmission enhancement produced by melittin was unaffected by the 5-hydroxytryptamine 3 receptor and P2X receptor antagonists 930 and pyridoxalphosphate-6-azophenyl-2=,4=-disulphonic acid, respectively). Nicotinic and muscarinic acetylcholine receptor agonists [(Ϫ)-nicotine and carbamoylcholine, respectively] and norepinephrine, as well as melittin, increased GABAergic sIPSC frequency and amplitude. A repeated application of (Ϫ)-nicotine, carbamoylcholine, and norepinephrine, but not melittin, at an interval of 30 min produced a similar transmission enhancement. These results indicate that melittin produces the release of acetylcholine and norepinephrine, which activate (nicotinic and muscarinic) acetylcholine and ␣ 1 -adrenergic receptors, respectively, resulting in GABAergic but not glycinergic transmission enhancement in SG neurons.

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Effect of resiniferatoxin on glutamatergic spontaneous excitatory synaptic transmission in substantia gelatinosa neurons of the adult rat spinal cord

Cited by 31 publications

References 57 publications

Genotype-dependent responsivity to inflammatory pain: A role for TRPV1 in the periaqueductal grey

Genotype-dependent responsivity to inflammatory pain: A role for TRPV1 in the periaqueductal grey

Spontaneous l-glutamate release enhancement in rat substantia gelatinosa neurons by (−)-carvone and (+)-carvone which activate different types of TRP channel

Acetylcholine and norepinephrine mediate GABAergic but not glycinergic transmission enhancement by melittin in adult rat substantia gelatinosa neurons

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