Effect of resistance selection on activities of detoxification enzymes and digestive enzymes in &lt;I&gt;Plutella xylostella&lt;/I&gt;

Liu, Yang; Liu, Shuangqing; Gong, Lei; Zhang, Huichao; JiCai, Tan; Song-qing, Huang; ZhongXia, Yang

doi:10.3724/sp.j.1238.2013.00529

Cited by 1 publication

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“…These insect detoxification enzymes can be individually or synergistically implicated in the detoxification metabolism of different insecticides [17]. Yang et al found that the activities of glutathione S-transferases (GSTs), carboxylesterase (CarE) and acetylcholinesterase (AChE) in the resistant strain were higher than those in the susceptible strain [18].…”

Section: Introductionmentioning

confidence: 99%

Glutathione S-Transferase May Contribute to the Detoxification of (S)-(−)-Palasonin in Plutella xylostella (L.) via Direct Metabolism

Fan

Liu

et al. 2022

Insects

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The control of P. xylostella primarily involves chemical insecticides, but overuse has brought about many negative effects. Our previous study reported that (S)-(−)-palasonin (PLN) is a plant-derived active substance with significant insecticidal activity against P. xylostella. However, we noticed a possible cross-resistance between (S)-(−)-palasonin and other insecticides which may be related to metabolic detoxification. In order to further explore the detoxification effect of detoxification enzymes on (S)-(−)-palasonin in P. xylostella, the effects of (S)-(−)-palasonin on enzyme activity and transcription level were determined, and the detoxification and metabolism of GSTs on (S)-(−)-palasonin were studied by in vitro inhibition and metabolism experiments. During this study, GST enzyme activity was significantly increased in P. xylostella after (S)-(−)-palasonin treatment. The expression levels of 19 GSTs genes were significantly increased whereas the expression levels of 1 gene decreased. Furthermore, (S)-(−)-palasonin is shown to be stabilized with GSTs and metabolized GSTs (GSTd1, GSTd2, GSTs1 and GSTs2) in vitro, with the highest metabolic rate of 80.59% for GSTs1. This study advances the beneficial utilization of (S)-(−)-palasonin as a botanical pesticide to control P. xylostella in the field.

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Section: Introductionmentioning

confidence: 99%