Effect of resveratrol incorporated in liposomes on proliferation and UV-B protection of cells

Caddeo, Carla; Teskač, Karmen; Sinico, Chiara; Kristl, Julijana

doi:10.1016/j.ijpharm.2008.07.024

Cited by 199 publications

(115 citation statements)

References 32 publications

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“…The preservation of the antioxidant activity after three months storage in nanoencapsulated form suggests that lipidic nanoemulsions can present a solution to the problem of instability of resveratrol, which was reported to be easily oxidized (Frozza et al, 2011). This also came in accordance with several other authors (Lucas-Abellán et al, 2007;Caddeo et al, 2008;Coimbra et al, 2011;Sessa et al, 2014), who declared that encapsulation of resveratrol in carriers, such as cyclodextrin complexes, liposomes and lecithin-based nanoemulsions were able to improve the stability of resveratrol. Similarly, this could be applied to curcumin, in which authors reported that its encapsulation in nanoformulations improves its stability (Rachmawati et al, 2015).…”

Section: Stability Of the Selected Nanoemulsion Formulasupporting

confidence: 86%

Development of an optimized hyaluronic acid-based lipidic nanoemulsion co-encapsulating two polyphenols for nose to brain delivery

Nasr

2015

Drug Delivery

170

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RESEARCH ARTICLEDevelopment of an optimized hyaluronic acid-based lipidic nanoemulsion co-encapsulating two polyphenols for nose to brain delivery Maha NasrDepartment of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt AbstractThe development of mucoadhesive lipidic nanoemulsion based on hyaluronic acid, co-encapsulating two polyphenols (resveratrol and curcumin) for the transnasal treatment of neurodegenerative diseases was attempted in the current manuscript. Nanoemulsions were prepared by the spontaneous emulsification method, and were characterized for their particle size, zeta potential, mucoadhesive strength and morphology. The selected formula was tested for its antioxidant potential, in vitro and ex vivo release of the two polyphenols, safety on nasal mucosa and in vivo quantification of the two drugs in rat brains. Its stability was tested by monitoring the change in particle size, zeta potential, drugs' content and antioxidant potential upon storage for 3 months. The optimized hyaluronic acid based nanoemulsion formula displayed a particle size of 115.2 ± 0.15 and a zeta potential of À23.9 ± 1.7. The formula displayed a spherical morphology and significantly higher mucoadhesive strength compared to its non mucoadhesive counterpart. In addition, the nanoemulsion was able to preserve the antioxidant ability of the two polyphenols and protect them from degradation. Diffusion controlled release of the two drugs was achievable till 6 hours, with an ex vivo flux across sheep nasal mucosa of 2.86 and 2.09 mg/cm 2 hr for resveratrol and curcumin, respectively. Moreover, the mucoadhesive nanoemulsion was safe on nasal mucosa and managed to increase the amounts of the two polypehnols in the brain (about 7 and 9 folds increase in AUC 0-7 h for resveratrol and curcumin, respectively). Hyaluronic acid based lipidic nanoemulsion proved itself as a successful carrier enhancing the solubility, stability and brain targetability of polyphenols.

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Section: Stability Of the Selected Nanoemulsion Formulasupporting

confidence: 86%

Development of an optimized hyaluronic acid-based lipidic nanoemulsion co-encapsulating two polyphenols for nose to brain delivery

Nasr

2015

Drug Delivery

170

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“…Large negative or positive zeta potential values induce repulsive interaction between the vesicles and thus avoiding aggregation of vesicles. Accordingly in the present study, the zeta potential of all the formulations was found to be less than À30 mV, which is considered as an acceptable value for physical stability of the vesicles (Caddeo et al, 2008). Amounts of EGCG and HA were optimized to be 10 mg and 1.75 mg respectively for 100 mg of lipid (ETF20) by considering the vesicle size, zeta potential and percentage of EE.…”

Section: Effect Of Drug Concentrationmentioning

confidence: 65%

Skin delivery of epigallocatechin-3-gallate (EGCG) and hyaluronic acid loaded nano-transfersomes for antioxidant and anti-aging effects in UV radiation induced skin damage

et al. 2017

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(2017) Skin delivery of epigallocatechin-3-gallate (EGCG) and hyaluronic acid loaded nano-transfersomes for antioxidant and anti-aging effects in UV radiation induced skin damage, Drug Delivery, 24:1, 61-74, DOI: 10.1080/10717544.2016 AbstractThe present work attempts to develop and statistically optimize transfersomes containing EGCG and hyaluronic acid to synergize the UV radiation-protective ability of both compounds, along with imparting antioxidant and anti-aging effects. Transfersomes were prepared by thin film hydration technique, using soy phosphatidylcholine and sodium cholate, combined with high-pressure homogenization. They were characterized with respect to size, polydispersity index, zeta potential, morphology, entrapment efficiency, Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), X-ray Diffraction (XRD), in vitro antioxidant activity and ex vivo skin permeation studies. Cell viability, lipid peroxidation, intracellular ROS levels and expression of MMPs (2 and 9) were determined in human keratinocyte cell lines (HaCaT). The composition of the transfersomes was statistically optimized by Design of Experiments using Box-Behnken design with four factors at three levels. The optimized transfersome formulation showed vesicle size, polydispersity index and zeta potential of 101.2 ± 6.0 nm, 0.245 ± 0.069 and À44.8 ± 5.24 mV, respectively. FTIR and DSC showed no interaction between EGCG and the selected excipients. XRD results revealed no form conversion of EGCG in its transfersomal form. The optimized transfersomes were found to increase the cell viability and reduce the lipid peroxidation, intracellular ROS and expression of MMPs in HaCaT cells. The optimized transfersomal formulation of EGCG and HA exhibited considerably higher skin permeation and deposition of EGCG than that observed with plain EGCG. The results underline the potential application of the developed transfersomes in sunscreen cream/lotions for improvement of UV radiation-protection along with deriving antioxidant and anti-aging effects.

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“…This study indicates that resveratrol is much more photoprotective when used in low dose. In an another study, Howitz et al (2003) showed that the photoprotective effect of resveratrol from radiation induced apoptosis in HEK 293 cells was reversed at concentrations greater than 50 (M. Caddeo et al (2008) also showed that at low concentration, resveratrol increased the number of cells without any evidence of toxicity. In contrast, cells treated with higher concentration of resveratrol showed loss of membrane integrity due to polymerized actin degradation and impaired functional status of cell (Caddeo et al 2008).…”

Section: Dose Dependency Of Resveratrolmentioning

confidence: 97%

Dose-Dependency of Resveratrol in Providing Health Benefits

2010

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Effect of resveratrol incorporated in liposomes on proliferation and UV-B protection of cells

Cited by 199 publications

References 32 publications

Development of an optimized hyaluronic acid-based lipidic nanoemulsion co-encapsulating two polyphenols for nose to brain delivery

Development of an optimized hyaluronic acid-based lipidic nanoemulsion co-encapsulating two polyphenols for nose to brain delivery

Skin delivery of epigallocatechin-3-gallate (EGCG) and hyaluronic acid loaded nano-transfersomes for antioxidant and anti-aging effects in UV radiation induced skin damage

Dose-Dependency of Resveratrol in Providing Health Benefits

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