Effect of rhPDGF-BB Delivery on Mediators of Periodontal Wound Repair

Abstract

Growth factors such as platelet-derived growth factor (PDGF) exert potent effects on wound healing including the regeneration of tooth-supporting structures. This investigation examined the effect of the local delivery of PDGF-BB when combined with reconstructive periodontal surgery on local wound fluid (WF) levels of PDGF-AB, vascular endothelial growth factor (VEGF), and bone collagen telopeptide (ICTP) in humans with advanced periodontitis. Sixteen patients exhibiting localized periodontal osseous defects were randomized to one of three groups (beta-TCP carrier alone, beta-TCP + 0.3 mg/mL of recombinant human PDGF-BB [rhPDGF-BB], or beta-TCP + 1.0 mg/mL of rhPDGF-BB) and monitored for 6 months. WF was harvested and analyzed for PDGF-AB, VEGF, and ICTP WF levels. Teeth contralateral to the target lesions served as controls. Increased levels of VEGF in the WF was observed for all surgical treatment groups with the 1.0 mg/mL rhPDGF-BB group showing the most pronounced difference at 3 weeks in the AUC analysis versus control (p < 0.0001). PDGF-AB WF levels were increased for the carrier alone group compared to both rhPDGFBB groups. Low-dose rhPDGF-BB application elicited increases in ICTP at days 3-5 in the wound healing process, suggesting a promotion of bone turnover at early stages of the repair process (p < 0.02). These results demonstrate contrasting inducible expression patterns of PDGF-AB, VEGF, and ICTP during periodontal wound healing in humans.

Influence of Aging on Biological Properties of Periodontal Ligament Cells

“…The results of the present investigation demonstrated no clear effect of aging on PDGF-1 and bFGF expression. PDGF-1 and bFGF are potent mitogenic and angiogenic factors that play key roles in periodontal wound repair [37,38] and, similarly to the present study, previous investigations have suggested that cellular aging may not act through alterations on the expression of growth factors or the number of cell-surface receptors [39]. Aged cells seem to become increasingly refractory to mitogenic and regulatory signals; however, the mechanisms underlying the hyporesponsiveness to mitogenic stimuli remain controversial.…”

The effect of Emdogain® and platelet-derived growth factor on the osteoinductive potential of hydroxyapatite tricalcium phosphate

“…PDGF-BB may enhance angiogenesis by directly effecting pericytes, vSMCs, and endothelial cells [63][64][65][66] or indirectly by promoting the production of angiogenic growth factors such as VEGF [67,68] or FGF2 [69,70]. It is possible that the angiogenic effects demonstrated in the PDGF group are mediated directly by increased local concentrations of PDGF-BB or indirectly via endogenous VEGF and FGF2.…”

Regeneration of Dental-Pulp-like Tissue by Chemotaxis-Induced Cell Homing

“…21,22 Platelet-derived growth factor (PDGF) at a dose of 10 ng/mL (R&D) was adopted for its roles in promoting angiogenesis, especially sprouting of existing blood vessels. 23,24 Nerve growth factor (NGF) at a dose of 50 ng/mL (R&D) was adopted for its roles in promoting the survival and growth of nerve fibers. 25 Bone morphogenetic protein-7 (BMP7) at a dose of 100 ng/mL was adopted for its roles in promoting mineralized tissue formation.…”