Effect of riboflavin-butyrate on cardiac glutathione reductase affected by adriamycin.

Abstract

SummaryMale Wistar rats received intraperitoneal injections of adriamycin (4mg/kg body weight/day) and/or riboflavin-butyrate (20mg/kg body weight/day) for 6 consecutive days. Cardiac mitochondria were then prepared for our present experiment. The combined use of riboflavin-butyrate with adriamycin was evaluated for reduction of lipid peroxide formation in rat cardiac mitochondria. In order to find the mechanism of the effect of riboflavin-butyrate, the glutathione peroxidase glutathione reductase system was examined. Adriamycin reduced the glutathione reductase activity in rat cardiac mitochondria, but did not affect the glutathione peroxidase activity. The mitochondrial content of flavin adenine dinucleotide, a prosthetic group of glutathione reductase, was greatly reduced and apoprotein of glutathione reductase also de creased. The administration of riboflavin-butyrate with adriamycin in creased flavin adenine dinucleotide and glutathione reductase activity. These results suggest that exogenous administration of riboflavin-butyrate is capable of reducing lipid peroxide by both enzymatic detoxification through glutathione reductase and non-enzymatic detoxification due to direct reaction with lipid peroxide.

Effects of Dietary Supplementation with Vitamin E, Riboflavin and Selenium on Central Nervous System Oxygen Toxicity

“…There is some evidence suggesting that dietary supplementation with Se, vitamin E (VIT E, a-tocopherol) and riboflavin (RIB), under certain oxidative stresses (e.g., treatment with paraquat or adriamycin) may afford protection against substantial loss of cellular GSH levels (Chung & Maines 1981;Hino et al 1985;Taniguchi & Hara 1983). Yet, the available information on the potential benefit of the above nutrients as regards exposure of the CNS to HBO are not conclusive (Jamieson 1989).…”

Riboflavin Deficiency Caused by Treatment with Adriamycin.

“…However, adriamycin produces a dose-dependent cardiotoxicity that restricts its clinical use in chemotherapy. We reported that intraperitoneal administration of adriamycin induces the depletion of flavin content in rat heart mitochondria (1). The combination use of adriamycin with riboflavin was found to be effective in preventing heart mitochondrial disorders in rats treated with adriamycin (1).…”

“…We reported that intraperitoneal administration of adriamycin induces the depletion of flavin content in rat heart mitochondria (1). The combination use of adriamycin with riboflavin was found to be effective in preventing heart mitochondrial disorders in rats treated with adriamycin (1). Therefore, the depletion of riboflavin in tissues is considered to be closely related to the administration of adriamycin .…”