Effect of Rice AUXIN BINDING PROTEIN57 (OsABP57) Overexpression in Response to Flooding

Zambrose, Zarith Ayunie; Roszelin, Sitti' Aisyah Mohd; Hazbir, Nur Aminah Binti Mohd; Chew, Bee Lynn; Jumali, Siti Sarah; Yahya, Wan Asrina Wan; Isa, Nurulhikma Md

doi:10.17265/2161-6264/2020.03.001

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…Image J allows the measurement of density profiles, peak heights as well and peak intensity of the band of the expected molecular weight. Gene expression is relative to the loading control gene from the control plant (without treatment) and normalized to the internal constitutive gene (target gene × (loading gene (control)/internal constitutive gene)) (Zambrose et al, 2020). The fold change value was generated from the normalized value.…”

Section: Densitometry Analysismentioning

confidence: 99%

Characterization And Functional Study Of Stress-Associated Protein In Rice And Arabidopsis

Mohd Roszelin,

Mohd Hazbir,

Jumali

et al. 2023

MAB

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Environmental stress can hinder the growth and development of crops, thereby reducing productivity. Plants can adapt to changing environments through various morpho-physiological changes, transcriptome regulation, signaling, translational and post-translational modifications. Stress Associated Proteins (SAPs) have been shown to play a crucial role in plant adaptation to biotic and abiotic stressors. They are encoded by a family of genes that produce a zinc finger protein with A20 and/or AN1 domains at either their N or C-terminal ends. Therefore, this study focused on understanding the role of the Oryza sativa SAP gene family (OsSAPs) in response to drought and salinity stress. In-silico analysis revealed that most of the OsSAP family members were upregulated by stress; two highly inducible OsSAP genes were also upregulated in response to stress under a rice-specific background. To study gene function, an Arabidopsis transformation system was employed using three genotypes: Col-0 (wild type), overexpressed transgenic OsSAP8, and atsap2 T-DNA knockout mutant. Arabidopsis AtSAP2 gene, which is homologous to rice OsSAP8, was used as a comparison to the loss of function mutation in Arabidopsis. Morphophysiological analysis showed that the atsap2 mutant displayed a sensitive phenotype to drought and salinity stress through low relative chlorophyll content and delayed inflorescence development and flowering as compared to Col-0 and transgenic OsSAP8. This suggests that the abolished atsap2 gene may contribute to reduced stress tolerancein plants. In contrast, transgenic OsSAP8 overexpression demonstrated tolerance to drought and salinity stress by maintaining relative chlorophyll content under both stress conditions, indirectly reflecting sustained photosynthetic machinery and stable photosynthetic rate. Further investigation, such as measuring the photosynthesis rate, is required to establish the correlation between chlorophyll data and photosynthesis activity.

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Section: Densitometry Analysismentioning

confidence: 99%

Characterization And Functional Study Of Stress-Associated Protein In Rice And Arabidopsis

Mohd Roszelin,

Mohd Hazbir,

Jumali

et al. 2023

MAB

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“…Previously, a study showed that the ABP57 overexpression had increased the resistance of transgenic rice to drought stress (23). Moreover, the 35S::ABP57 transgenic Arabidopsis possessed root elongation changes and also restoration of root cells under drought and submergence conditions (24)(25)(26).…”

Section: Introductionmentioning

confidence: 99%

In silico promoter and expression analyses of rice Auxin Binding Protein 57 (ABP57)

et al. 2021

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Auxin Binding Protein 57 (ABP57) is one of the molecular components involved in rice response to abiotic stress. The ABP57 gene encodes an auxin receptor which functions in activating the plasma membrane H+-ATPase. Biochemical properties of ABP57 have been characterized; however, the function of ABP57, particularly on stress and hormone responses is still limited. This study was conducted to understand the regulation of ABP57 expression under abiotic stress. Thus, in silico identification of cis-acting regulatory elements (CAREs) in the promoter region of ABP57 was performed. Several motifs and transcription factor binding site (TFBS) that are involved in abiotic stress such as ABRE, DRE, AP2/EREBP, WRKY and NAC were identified. Next, expression analysis of ABP57 under drought, salt, auxin (IAA) and abscisic acid (ABA) was conducted by reverse transcription-PCR (RT-PCR) to verify the effect of these treatments on ABP57 transcript level. ABP57 was expressed at different levels in the shoot and root under drought conditions, and its expression was increased under IAA and ABA treatments. Moreover, our results showed that ABP57 expression in the root was more responsive to drought, auxin and ABA treatments compared to its transcript in the shoot. This finding suggests that ABP57 is a drought-responsive gene and possibly regulated by IAA and ABA.

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Effect of Rice AUXIN BINDING PROTEIN57 (OsABP57) Overexpression in Response to Flooding

Cited by 2 publications

References 21 publications

Characterization And Functional Study Of Stress-Associated Protein In Rice And Arabidopsis

Characterization And Functional Study Of Stress-Associated Protein In Rice And Arabidopsis

In silico promoter and expression analyses of rice Auxin Binding Protein 57 (ABP57)

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