Effect of river flow, temperature, and water chemistry on proliferations of the benthic anatoxin-producing cyanobacterium <i>Phormidium</i>

Wood, Susanna A.; Atalah, Javier; Wagenhoff, Annika; Brown, L. L.; Doehring, Kati; Young, Roger G.; Hawes, Ian

doi:10.1086/690114

Cited by 35 publications

(45 citation statements)

References 54 publications

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“…Most scientists investigating Phormidium dynamics at a reach scale have suggested that river flow and watercolumn NO 3 2 play a role in determining its cover (Heath et al 2011, Wood et al 2017, McAllister et al 2018), but the responses of individual patches to NO 3 2 and velocity have not been assessed experimentally. We seeded cobbles with a standardized inoculum of Phormidium, which al-lowed high replication and examination of the effects of velocity and NO 3 2 on the later 2 stages of the accrual cycle: expansion and detachment.…”

Section: Discussionmentioning

confidence: 99%

“…N was not limiting growth and other factors like light or temperature, which were relatively low throughout the experimental period, were limiting growth. Wood et al (2017) highlighted that when DIN concentrations were <0.8 mg/L, DIN and Phormidium cover were positively related. Loza et al (2014) found in a culture study that Phormidium responded positively to NO 3 2 up to concentrations of 100 mg/L.…”

Section: Phormidium Biomass Accrualmentioning

confidence: 97%

“…3 for treatment abbreviations. that N fixation could have been occurring (Heath et al 2014, Wood et al 2017, McAllister et al 2018.…”

Section: Phormidium Biomass Accrualmentioning

confidence: 99%

“…In New Zealand, proliferations of Phormidium consistently occur at dissolved reactive P (DRP) concentrations <0.01 mg/L , 2017, McAllister et al 2016, whereas in Spain, Phormidium autumnale is prevalent in hypertrophic systems (Loza et al 2013). Wood et al (2017) suggested a lower limit of watercolumn dissolved inorganic N (DIN) concentrations for proliferation of Phormidium of~0.1 mg/L, but proliferations have been observed in NO 3 2 concentrations <0.02 mg/L (Wood et al 2017, McAllister et al 2018. The ability to accumulate biomass under low nutrient concentrations is a well-established trait of benthic microbial mats (Bonilla et al 2005).…”

mentioning

confidence: 99%

“…The ability to accumulate biomass under low nutrient concentrations is a well-established trait of benthic microbial mats (Bonilla et al 2005). Investigators have suggested that elevated NO 3 2 concentrations enhance Phormidium growth dur-ing early accrual stages, but that internal mat nutrientcycling processes in established mats allow it to grow independently of water-column nutrient concentrations (McAllister et al 2016, 2018, Wood et al 2017. Studies of the effect of NO 3 2 on Phormidium growth have been limited to laboratory-culture-based (Heath et al 2014 and observational studies (Heath et al 2011, Wood et al 2017, McAllister et al 2018.…”

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confidence: 99%

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The effects of velocity and nitrate on Phormidium accrual cycles: a stream mesocosm experiment

et al. 2018

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Proliferations of benthic cyanobacteria in the genus Phormidium are a global concern because of their increasing prevalence and ability to produce harmful toxins. Most studies have been observational and have linked physicochemical variables to Phormidium cover measured at the reach scale. Authors of these studies have alluded to nutrients and flow as key factors in accrual. Our goal was to use an experimental approach to examine how changes in velocity and NO 3 2 concentrations influence Phormidium accrual. We hypothesized that: 1) Phormidium biomass accrual would be positively correlated with stream velocity; 2) biomass accrual would be positively related to NO 3 2 concentration, which would have a stronger effect during early accrual; 3) an NO 3 2 Â velocity interaction would arise from saturation of accrual at high NO 3 2 and high velocity; and 4) the probability of detachment would increase with decreasing velocity. We assessed mat expansion, biomass (as phycoerythrin and chlorophyll a [Chl a] concentrations, and biovolume), and algal assemblage composition in flow-through channel mesocosms for 16 d. We crossed 2 velocity treatments (0.1 and 0.2 m/s, slow and fast, respectively) with 3 NO 3 2 treatments (0.02, 0.1, and 0.4 mg/L, ambient, medium, and high, respectively). Velocity was positively correlated with all measures of Phormidium biomass, but patch expansion rates increased at similar rates across all treatments. NO 3 2 had no effect during early accrual, but phycoerythrin concentrations increased with increasing NO 3 2 in fast-velocity treatments. At the end of the experiment, patch size was greater in the high-velocity treatments because of a greater number of partial or full patch detachments in slow-velocity treatments. These results suggest that NO 3 2 concentrations do not affect Phormidium expansion and detachment, but may be important during colonization (not investigated), and that mat expansion occurs at a similar rate regardless of velocity, but termination of accrual cycles occurs earlier in slow velocities.

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Section: Discussionmentioning

confidence: 99%

Section: Phormidium Biomass Accrualmentioning

confidence: 97%

“…3 for treatment abbreviations. that N fixation could have been occurring (Heath et al 2014, Wood et al 2017, McAllister et al 2018.…”

Section: Phormidium Biomass Accrualmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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The effects of velocity and nitrate on Phormidium accrual cycles: a stream mesocosm experiment

et al. 2018

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Diversity of freshwater algal assemblages across the United States as revealed by DNA metabarcoding

Schulte,

Craine,

Leopold

et al. 2023

Environmental DNA

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As freshwater algae respond strongly to environmental conditions, algal communities are routinely used as indicators of aquatic health. Algal bioassessments have historically relied upon microscopy‐based identifications that are typically slow, expensive, taxonomically restricted, and inconsistent across analysts and time. Metabarcoding of water column DNA (environmental DNA, or eDNA) can characterize assemblages more quickly, at lower cost, and with higher taxonomic precision than microscopy. As such, eDNA metabarcoding has the potential to improve bioassessments, but relationships between environmental conditions and eDNA‐derived algal assemblage composition need to be determined first. We performed metabarcoding of a plastid 23S rRNA gene region for 1230 freshwater eDNA samples collected from 51 lakes and 617 streams across the conterminous United States to test for assemblage‐wide patterns that may indicate ecological condition. Samples were collected by citizen, academic, and research scientists using a standardized commercial kit. This effort constitutes the largest published water column eDNA survey yet of algal diversity across freshwaters in the United States. We detected 14,943 algal exact sequence variants (ESVs) from 11 divisions. The richness and abundance of cyanobacteria was higher in lakes, while streams were dominated by diatoms. Nationwide, only 1% of variation in stream assemblages was explained by catchment integrity. The remaining, explicable 19% was associated with forest cover, stream order, elevation, and broad‐scale spatial variables. Nevertheless, select ESVs were candidate indicators of gradients in stream catchment integrity and possible eutrophication. Together, we show that algal eDNA metabarcoding has potential for measuring ecological condition relative to water quality. Yet, further sampling along anthropogenic gradients is needed before algal eDNA can be used for large‐scale biomonitoring in the United States. We also found that only 2% of algal ESVs could be assigned to U.S. morphospecies, highlighting the importance of building a more comprehensive reference sequence database to integrate existing morphospecies autecology with eDNA‐based bioassessments.

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In situ river experiments to explore variability in Microcoleus autumnalis mat expansion

et al. 2020

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Effect of river flow, temperature, and water chemistry on proliferations of the benthic anatoxin-producing cyanobacterium Phormidium

Cited by 35 publications

References 54 publications

The effects of velocity and nitrate on Phormidium accrual cycles: a stream mesocosm experiment

The effects of velocity and nitrate on Phormidium accrual cycles: a stream mesocosm experiment

Diversity of freshwater algal assemblages across the United States as revealed by DNA metabarcoding

In situ river experiments to explore variability in Microcoleus autumnalis mat expansion

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