Effect of root applied 24-epibrassinolide on carbohydrate status and fermentative enzyme activities in cucumber (Cucumis sativus L.) seedlings under hypoxia

Kang, Yunyan; Guo, Shirong; Li, Juan; JiuJu, Duan

doi:10.1007/s10725-008-9344-x

Cited by 44 publications

(27 citation statements)

References 40 publications

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“…In this regard, pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH) are considered as important plant proteins for coping with hypoxia-induced cellular damage [81]. According to Kang et al [82], 24-epibrassinolide (EBR) further enhances ADH activity in hypoxic cucumber roots. Therefore, PDC and ADH were analyzed by western blotting to verify the proteomic data.…”

Section: Resultsmentioning

confidence: 99%

Proteomic analysis of the effects of exogenous calcium on hypoxic-responsive proteins in cucumber roots

Xin-shi

Tian

et al. 2012

Proteome Sci

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BackgroundHypoxia acts as a plant stress factor, particularly in cucumbers plants under hydroponic culture. Calcium is involved in stress signal transmission and in the growth of plants. To determine the effect of exogenous calcium on hypoxic-responsive proteins in cucumber (Cucumis sativus L. cv. Jinchun No.2) roots, proteomic analysis was performed using two-dimensional electrophoresis (2-DE) and mass spectrometry.ResultsCucumber roots were used to analyze the influence of hypoxia on plants. The expressions of 38 protein spots corresponding to enzymes were shown to change in response to hypoxia. Of these, 30 spots were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/TOF MS analysis). The proteins were categorized according to functional groups, including glycolysis, the tricarboxylic acid (TCA) cycle, fermentative metabolism, nitrogen metabolism, energy metabolism, protein synthesis and defense against stress. Exogenous calcium appeared to alleviate hypoxic stress via these metabolic and physiological systems. Western blotting was used to analyze the accumulation of alcohol dehydrogenase (ADH) and pyruvate decarboxylase (PDC); calcium further increased the expression of ADH and PDC under hypoxia. In addition, semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to assess the transcript levels of differentially expressed proteins.ConclusionsExogenous calcium enhanced the expression of enzymes involved in glycolysis, the TCA cycle, fermentative metabolism, nitrogen metabolism, and reactive oxygen species (ROS) defense in plants under hypoxia. Calcium appears to induce hypoxic tolerance of cucumber seedlings. These phenomena have prompted us to further investigate the mechanisms by which cucumbers respond to exogenous calcium under hypoxia.

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Section: Resultsmentioning

confidence: 99%

Proteomic analysis of the effects of exogenous calcium on hypoxic-responsive proteins in cucumber roots

Xin-shi

Tian

et al. 2012

Proteome Sci

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“…Kang et al [27] found that the total soluble and starch content of cucumber leaves increased significantly under hypoxic stress because of decreased sugar transport from leaves to roots. Our previous study also found that root-zone hypoxic stress decreased the biomass of plants and soluble protein of leaves [33].…”

Section: Discussionmentioning

confidence: 99%

The effect of exogenous calcium on cucumber fruit quality, photosynthesis, chlorophyll fluorescence, and fast chlorophyll fluorescence during the fruiting period under hypoxic stress

et al. 2018

BMC Plant Biol

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BackgroundPlants often suffer from hypoxic stress during waterlogging and hydroponic culturing. This study investigated the response of cucumber (Cucumis sativus L.) plant growth parameters, leaf photosynthesis, chlorophyll fluorescence, fast chlorophyll a fluorescence transient (OJIP), and fruit quality parameters to hypoxic stress alleviated by exogenous calcium. During the fruiting period, cucumber plants were exposed to hypoxia and hypoxia + Ca2+ treatment (4 mM Ca2+) for 9 d.ResultExogenous calcium application enhanced the biomass and fruit quality of hypoxic stressed cucumber and also increased the net photosynthesis rate, stomatal conductance, intercellular CO2 concentration, maximum quantum efficiency of photosystem II photochemistry, actual photochemical efficiency of PSII, photochemical quenching coefficient, and non-photochemical quenching coefficient. Additionally, measurement of chlorophyll a fluorescence transients showed the positive K- and L-bands were more pronounced in leaves treated with hypoxia compared with those with hypoxia + Ca2+, indicating that hypoxic treatment induced uncoupling of the oxygen-evolving complex and inhibited electron transport beyond plastoquinone pool (Qa, Qb) including possible constraints on the reduction of end electron acceptors of photosystem I. Exogenous calcium can reduce these stress-induced damages in cucumber.ConclusionThis research focused the effect of exogenous calcium on cucumber photosynthesis during the fruiting period under hypoxic stress. Hypoxic stress might impair the photosynthetic electron-transport chain from the donor side of PSII up to the reduction of end acceptors of PSI, and exogenous calcium enhanced electron transport capacity and reduced hypoxic damage of cucumber leaves.

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“…And the previous results showed that exogenous application of BRs to leaves of salt-stressed cotton plants regulated leaf physiology and had little effect on the roots (Shu et al 2011). Kartal et al (2009) found that homobrassinolide (HBL) increased primary root growth during barley germination stage, and Kang et al (2009) reported that root-applied epibrassinolide (EBL) alleviated oxidative damage induced by root-zone hypoxia in cucumber seedlings. Therefore, a root application of BRs might be the most effective to improve cotton salt tolerance.…”

Section: Introductionmentioning

confidence: 99%

Root-applied brassinolide can alleviate the NaCl injuries on cotton

et al. 2015

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Brassinolide (BL) is a plant growth regulator. This study analyzes the effects of BL on cotton growth, Na ? accumulation, proline content, MDA content, antioxidant enzyme activities, and differentially expressed genes (DEGs) of cotton roots under NaCl stress. We grew two cotton cultivars, Sumian 12 (salt sensitive) and Sumian 22 (salt tolerant), in continuously aerated Hoagland's nutrient solution supplemented with 200 mM NaCl. NaCl stress increased the Na ? , proline, and MDA content and decreased root activity and protein content in the roots of Sumian 12 and 22, and the change rang of Sumian 12 was higher than that of Sumian 22. The application of BL counteracted the NaCl stress-induced growth inhibition in the two tested cotton cultivars. It reduced the accumulation of Na ? , enhanced proline content, and resulted in an overall change in the activities of antioxidant enzymes causing a decrease in the MDA content of NaCl-stressed roots, and the influence of BL on salt-stressed Sumian 12 plants was more pronounced than that on Sumian 22. The digital gene expression analysis in Sumian 12 indicated that BL application significantly influenced the gene expression in NaCl-stressed roots. The majority of 7659 (3661 up-regulated/3998 down-regulated) DEGs of NaCl/ CK in roots of Sumian 12 were regulated by BL, and the gene expression pattern as a result of the root-applied BL on NaCl-stressed cotton treatment (BL ? NaCl) was similar to the control. Our results indicate that the rootapplied brassinolide alleviates NaCl stress on cotton through improving root activity, physiology, and gene expression.

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Effect of root applied 24-epibrassinolide on carbohydrate status and fermentative enzyme activities in cucumber (Cucumis sativus L.) seedlings under hypoxia

Cited by 44 publications

References 40 publications

Proteomic analysis of the effects of exogenous calcium on hypoxic-responsive proteins in cucumber roots

Proteomic analysis of the effects of exogenous calcium on hypoxic-responsive proteins in cucumber roots

The effect of exogenous calcium on cucumber fruit quality, photosynthesis, chlorophyll fluorescence, and fast chlorophyll fluorescence during the fruiting period under hypoxic stress

Root-applied brassinolide can alleviate the NaCl injuries on cotton

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