Aim:The aim of study was radiographic assessment of the effect of conservative access cavity preparation and different root canal tapers preparation on apical transportation of curved root canals.Materials and methods: we used 45-extracted mandibular permanent molar having mesial root canal curvature range .CBCT done to determine the root curvature and anatomy for access cavity standardization. Teeth were selected and divided into three groups, according to access cavity preparation design into: Group1 (Traditional access cavity), Group2 (Conservative access cavity), Group3 (Truss access cavity). Pre instrumentation standardized periapical x-ray were done with #15 k-file inserted inside mesiobuccal canal Then teeth were divided according to rotary file system used for canal instrumentation into three subgroups ( Oneshape , ProTaperNext and Trunatomy). After access preparation done all teeth were instrumented using OneShape 25/0.6 , Protaper Next X1,X2 and Trunatomy small (#20) and Prime (#26) . Post instrumentation standardized radiograph were taken with (OneShape 25/0.6, ProTaper Next X2 and Trunatomy Prime (#26)) file inserted inside mesiobuccal canal. Superimposition of pre and post instrumentation radiographs were done using Adobe Photoshop program . Image-J software was used to measure angle of deviation between the 2 files. Statistical analysis for image analysis results was performed by applying ANOVA test (one way) followed by Tukey post hoc test.Results: Conservative access cavity showed highest angle of deviation when used with OneShape and Trunatomy while showed the lowest angle of deviation with ProTaper next .Regarding the type of file used, OneShape showed the highest angle of deviation with conservative and truss access cavities and lowest with traditional access cavity while ProTaper next showed the lowest angle of deviation with conservative and Truss access cavities .