The present investigation entitled effect of organic media, plant growth regulators and different colour of wrappers on success and survival of air layering of Guava (Psidium guajava L.) was carried out in Month of August during 2019 and 2020 at the Fruit Research Station Entkhedi, Bhopal, (M.P.) under the R.A.K. College of Agriculture, Sehore. The experiment was laid down in Randomized Block Design with three replication and twenty-five treatments. In these treatments five colour of Poly wrappers was used i.e.,White, Black,; Blue, Red and Green, five concentrations of Growth regulators i.e., IBA 0 ppm, IBA 2500 ppm, IBA 5000 ppm, IBA 7500 ppm and IBA 10000 ppm and four types of media i.e., Vermicompost, Soil, Leaf manure and Sphagnum moss. Observations was recorded Survival percentage of air-layers, Average number of new sprouts per layer, Average number of leaves per air layer and Average number of branches per air layers. Result obtained that the treatment T21 (IBA 10000 PPM + Vermicompost + W5) found superior in all the parameters of shoots characters and economics also during 2019-20 and 2020-21 also in pooled data.