Effect of rosemary supplementation on oxidative stress after myocardial infarction in rats

Abstract

ObjectiveTo analyze the influence of rosemary (R) consumption on cardiac oxidative stress after myocardial infarction (MI).MaterialMale Wistar rats weighing 200g were allocated into 6 groups: 1) Sham group fed standard chow (SA0, n = 6); 2) sham group fed standard chow supplemented with 0.02% of dried R (diet 1) (SA1, n = 6); 3) sham group fed standard chow supplemented with 0.2% of dried R (diet 2) (SA2, n = 6); 4) MI rats fed standard chow (IA0, n = 6); 5) MI rats fed diet 1 (IA1, n = 6); 6) MI rats fed diet 2 (IA2, n = 6). After three months of treatment, heart tissue was collected and tested for lipid hydroperoxide (LH) and antioxidant enzymes catalase, gluthatione peroxidase (GPx), superoxide dismutase (SOD) and Nrf2 expression. Comparisons were made by two‐way ANOVA test and post‐test of Holm‐Sidak. Values were presented as mean ± standard error.ResultsMI rats showed higher cardiac levels of LH and SOD than Sham rats. Rosemary supplementation reduced LH with diet 2 and SOD with both doses. No differences were observed for catalase, GPx and Nrf2.ConclusionChow supplementation with rosemary reduced oxidative stress after myocardial infarction. Financial support: FAPESP.