Effect of route of administration of dinoprost tromethamine on plasma profiles of 13,14-dihydro-15-keto-prostaglandin F2α and progesterone in lactating Holstein cows

Abstract

Our objective was to determine the effect of route of administration of dinoprost tromethamine 7 d after treatment with GnRH on circulating 13,14-dihydro-15-keto-prostaglandin F2α (PGFM) and progesterone (P4) concentrations in lactating dairy cows. Multiparous Holstein cows fitted with indwelling jugular catheters were randomized 7 d after the last GnRH of an Ovsynch protocol (G2, d 0) to receive 25 mg of dinoprost tromethamine either intramuscularly (IM) or subcutaneously (SC). The SC cows had greater circulating PGFM concentrations 15 to 90 min after treatment than the IM cows; however, circulating P4 concentrations during induced luteolysis did not differ at any time based on route of administration.

Comparison of Intra-CL Injection and Peripheral Application of Prostaglandin F2α Analog on Luteal Blood Flow and Secretory Function of the Bovine Corpus Luteum

“…Quality assurance of beef in particular seeks to minimize meat damage to preserve its value, by minimizing the use of hormones in beef production (40). The previous studies of Chebel et al (50) and Mezera et al (51), which focused on differences between SC and IM administration, indicated no differences in cows that underwent complete luteolysis. These authors suggested that the SC method of dinoprost administration is acceptable for CL regression.…”

“…Regulation of the estrous cycle is widely applied in veterinary practice for estrus synchronization. The most effective routes and sites for administration of PGF 2α analogs, used for manipulation of the reproductive processes in breeding cows, have been the subject of numerous studies ( 40 , 50 , 51 ). Besides IM administration of PGF 2α analogs, their subcutaneous injection (SC) in the cervical area, or SC injection in the ischio-rectal fossa have also produced declines in P 4 concentration, inducing luteolysis in the cow ( 40 , 50 , 51 ).…”

“…The most effective routes and sites for administration of PGF 2α analogs, used for manipulation of the reproductive processes in breeding cows, have been the subject of numerous studies ( 40 , 50 , 51 ). Besides IM administration of PGF 2α analogs, their subcutaneous injection (SC) in the cervical area, or SC injection in the ischio-rectal fossa have also produced declines in P 4 concentration, inducing luteolysis in the cow ( 40 , 50 , 51 ). Recently, we have noted that intra-CL injection of dinoprost reduced P 4 levels at 4 h in the middle-stage CL, whereas it did not change P 4 profiles in the early-stage CL ( 22 ).…”

“…The method of intra-CL application of drugs/hormones could be a prevalent tool in bovine reproduction. It is important to know that the dairy and beef cow industry economies depend on fertility, and this has been the subject of numerous studies ( 40 , 50 , 51 , 61 ). Quality assurance of beef in particular seeks to minimize meat damage to preserve its value, by minimizing the use of hormones in beef production ( 40 ).…”