Effect of royal jelly on experimental colitis Induced by acetic acid and alteration of mast cell distribution in the colon of rats

Abstract

This study investigated the effects of royal jelly (RJ) on acetic acid-induced colitis in rats. Twenty adult female Wistar albino rats were divided into four treatment groups of 5 animals each, including a control group (Group I); Group II was treated orally with RJ (150 mg kg−1 body weight); Group III had acetic acid-induced colitis; and Group IV had acetic acid-induced colitis treated orally with RJ (150 mg kg−1 body weight) for 4 weeks. Colitis was induced by intracolonic instillation of 4% acetic acid; the control group received physiological saline (10 mL kg−1). Colon samples were obtained under deep anaesthesia from animals in all groups. Tissues were fixed in 10% formalin neutral buffer solution for 24 h and embedded in paraffin. Six-micrometre-thick sections were stained with Mallory’s triple stain and toluidine blue in 1% aqueous solution at pH 1.0 for 5 min (for Mast Cells). RJ was shown to protect the colonic mucosa against the injurious effect of acetic acid. Colitis (colonic damage) was confirmed histomorphometrically as significant increases in the number of mast cells (MC) and colonic erosions in rats with acetic acid-induced colitis. The RJ treatment significantly decreased the number of MC and reduced the area of colonic erosion in the colon of RJ-treated rats compared with rats with untreated colitis. The results suggest that oral treatment with RJ could be used to treat colitis.

Royal jelly protects male rats from heat stress‐induced reproductive failure

“…The RJ contains vitamins such as E and C (Karaca et al, ) which have been reported that can reduce oxidative stress via MDA levels and superoxide dismutase and glutathione peroxidase activities improvement in diabetic patients erythrocytes (Pourmoradian, Mahdavi, Mobasseri, Faramarzi, & Mobasseri, ). Additionally, previous reports have indicated that RJ has membranoprotective effect during sperm freezing (Shahzad et al., ).…”

Exosome-like vesicles in Apis mellifera bee pollen, honey and royal jelly contribute to their antibacterial and pro-regenerative activity

“…Bee pollen, honey and royal jelly have demonstrated remarkable antimicrobial and pro-regenerative characteristics. Pre-clinical studies described an improvement of disease scores following royal jelly application in a number of conditions including mucositis, colitis, bone formation and infected ulcers (El-Gayar et al, 2016;Karaca et al, 2010;Özan et al, 2015;Suemaru et al, 2008). Furthermore, honey has been shown to be strongly antibacterial against various bacterial strains (e.g.…”

Therapeutic effect of adenosine on experimentally induced acute ulcerative colitis model in rats

“…By far, AA-induced ulcerative colitis has been used eminently in many experimental studies 3,7,9,14,15 . However, the effects of adenosine treatment in this model were not reported before.…”

“…Acute colitis was induced by rectal administration of 4% solution of acetic acid (AA) (Sigma Chemical Co.) in a volume of 1ml in 20 rats 14 . No drug was administered to the remaining 10 rats, assigned as the control group (n=10).…”