Effect of Rubisco Activase Deficiency on the Temperature Response of CO2 Assimilation Rate and Rubisco Activation State: Insights from Transgenic Tobacco with Reduced Amounts of Rubisco Activase

Yamori, Wataru; Caemmerer, Susanne von

doi:10.1104/pp.109.146514

Cited by 81 publications

(68 citation statements)

References 54 publications

(89 reference statements)

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“…At higher temperature, the steady-state photosynthesis in rice can be influenced by Rubisco activase. Yamori and von Cammerer (2009) reported that steady-state photosynthetic rate at higher temperature decreased in transgenic tobacco containing reduced Rubisco activase, which exhibited the same levels of photosynthesis as the wild-type at growth temperature. Since Rubisco activase limitation on photosynthesis may vary with the rice cultivar, it can be physiologically meaningful and also useful for improvement of photosynthetic efficiency in rice to analyze the difference in the potential of light-induction and heat tolerance of photosynthesis induced by Rubisco activase among various cultivars and wild relatives.…”

Section: Discussionmentioning

confidence: 99%

Photosynthetic Characteristics of Antisense Transgenic Rice Expressing Reduced Levels of Rubisco Activase

Masumoto

Fukayama

Hatanaka

et al. 2012

Plant Production Science

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Section: Discussionmentioning

confidence: 99%

Photosynthetic Characteristics of Antisense Transgenic Rice Expressing Reduced Levels of Rubisco Activase

Masumoto

Fukayama

Hatanaka

et al. 2012

Plant Production Science

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“…The frozen leaf sample was ground in liquid nitrogen and homogenized in an extraction buffer (Yamori and von Caemmerer, 2009;Yamori et al, 2010a). The content of Rieske FeS of Cyt b 6 /f complex and d-subunit of ATP synthase was quantified by immunoblotting with anti-Rieske FeS antibody and anti-ATP synthase (d) antibody (Agrisera).…”

Section: Gas Exchange and Fluorescence Measurementsmentioning

confidence: 99%

“…After gas exchange had reached the steady-state rate for at least 30 min at a given leaf temperature, the leaf section in the chamber was taken out and immediately frozen in liquid nitrogen. Rubisco catalytic sites and Rubisco activation state were determined by the stoichiometric binding of 14 C-carboxy-arabinitol-P 2 , whereas chloroplast NADP-MDH activation state was assayed by monitoring NADH oxidation at 340 nm (Yamori and von Caemmerer, 2009). …”

Section: Gas Exchange and Fluorescence Measurementsmentioning

confidence: 99%

“…The content of Rieske FeS of Cyt b 6 /f complex and d-subunit of ATP synthase was quantified by immunoblotting with anti-Rieske FeS antibody and anti-ATP synthase (d) antibody (Agrisera). Rubisco activase was also quantified by immunoblotting with anti-activase antibody (Yamori and von Caemmerer, 2009). Chl was extracted in 80% (v/v) acetone and determined (Porra et al, 1989).…”

Section: Gas Exchange and Fluorescence Measurementsmentioning

confidence: 99%

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The Roles of ATP Synthase and the Cytochrome b 6/f Complexes in Limiting Chloroplast Electron Transport and Determining Photosynthetic Capacity

et al. 2010

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In C 3 plants, CO 2 assimilation is limited by ribulose 1,5-bisphosphate (RuBP) regeneration rate at high CO 2 . RuBP regeneration rate in turn is determined by either the chloroplast electron transport capacity to generate NADPH and ATP or the activity of Calvin cycle enzymes involved in regeneration of RuBP. Here, transgenic tobacco (Nicotiana tabacum 'W38') expressing an antisense gene directed at the transcript of either the Rieske iron-sulfur protein of the cytochrome (Cyt) b 6 /f complex or the d-subunit of chloroplast ATP synthase have been used to investigate the effect of a reduction of these complexes on chloroplast electron transport rate (ETR). Reductions in d-subunit of ATP synthase content did not alter chlorophyll, Cyt b 6 /f complex, or Rubisco content, but reduced ETR estimated either from measurements of chlorophyll fluorescence or CO 2 assimilation rates at high CO 2 . Plants with low ATP synthase content exhibited higher nonphotochemical quenching and achieved higher ETR per ATP synthase than the wild type. The proportional increase in ETR per ATP synthase complex was greatest at 35°C, showing that the ATP synthase activity can vary in vivo. In comparison, there was no difference in the ETR per Cyt b 6 /f complex in plants with reduced Cyt b 6 /f content and the wild type. The ETR decreased more drastically with reductions in Cyt b 6 /f complex than ATP synthase content. This suggests that chloroplast ETR is more limited by Cyt b 6 /f than ATP synthase content and is a potential target for enhancing photosynthetic capacity in crops.Plants capture light energy with their light-harvesting systems, including chlorophylls (Chls) and carotenoids, and drive photosynthetic electron transport through the thylakoid membranes of the chloroplasts. Electrons excised from water in PSII are ultimately transferred to NADP + via PSI, resulting in production of NADPH. This process is known as linear electron transport. At the same time, this linear electron transport that passes through the cytochrome (Cyt) b 6 /f complex generates a proton gradient across the thylakoid membrane (DpH;Allen, 2003). Together with the proton gradient generated by the water-splitting complex associated with PSII, these proton gradients enable ATP production by the ATP synthase complex and help to regulate nonphotochemical quenching (NPQ) of excitation energy (Mü ller et al., 2001). There is also a cyclic electron transport that depends on the PSI photochemical reactions and also passes through the Cyt b 6 /f complex. The cyclic electron transport can generate a DpH and drives ATP synthesis by ATP synthase without concomitant generation of NADPH (Shikanai, 2007). ATP and NADPH generated by light reactions are utilized primarily in the Calvin cycle and photorespiratory cycle. The activity and regulation of the Cyt b 6 /f complex and the ATP synthase are thus key components determining the rate of NADPH and ATP production for CO 2 fixation. Photosynthetic CO 2 assimilation rate can be viewed as being limited either by the capacity o...

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“…Low rubisco activity at 25 • C might be due to the reduction in RuBP regeneration [7,25]. RuBP regeneration might be affected by the lower RuBP consumption by rubisco.…”

Section: Discussionmentioning

confidence: 99%

Photosynthesis of Sago Palm (Metroxylon sagu Rottb.) Seedling at Different Air Temperatures

et al. 2018

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Photosynthetic activities of the sago palm (Metroxylon sagu Rottb.) were studied to find out its sensitivity to changes in ambient air temperature. The minimum ambient air temperature designed for the experiment was 25-29 • C, while the higher end was 29-33 • C. Several photosynthetic parameters were studied to support our analysis in sago photosynthetic activity, including diurnal leaf gas exchange, assimilation rate vs. CO 2 concentration, leaf greenness, leaf chlorophyll content, and photosynthetic rate vs. irradiance. We found that sago palm photosynthetic activity tends to be more sensitive to minimum than to maximum ambient air temperature. The plants exposed to higher air temperatures had dark green leaf color associated with higher rates of diurnal photosynthesis, chlorophyll content, and rubisco limited photosynthetic activity. They also exhibited higher trend in optimum irradiance absorption level. Consequently, maximum light energy dissipation occurred at higher temperatures.

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Effect of Rubisco Activase Deficiency on the Temperature Response of CO2 Assimilation Rate and Rubisco Activation State: Insights from Transgenic Tobacco with Reduced Amounts of Rubisco Activase

Cited by 81 publications

References 54 publications

Photosynthetic Characteristics of Antisense Transgenic Rice Expressing Reduced Levels of Rubisco Activase

Photosynthetic Characteristics of Antisense Transgenic Rice Expressing Reduced Levels of Rubisco Activase

The Roles of ATP Synthase and the Cytochrome b 6/f Complexes in Limiting Chloroplast Electron Transport and Determining Photosynthetic Capacity

Photosynthesis of Sago Palm (Metroxylon sagu Rottb.) Seedling at Different Air Temperatures

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