Effect of rumen microbiota transfaunation on the growth, rumen fermentation, and microbial community of early separated Japanese Black cattle

Abstract

This study aimed to investigate the effect of rumen microbiota transfaunation on the growth, rumen fermentation, and the microbial community of Japanese Black cattle that were separated early from their dams. Here, 24 calves were separated from their dams immediately after calving, 12 of which were transfaunated via inoculation with rumen fluid from adult cattle at the age of 2 months while the remaining 12 were kept unfaunated (not‐inoculated). Feed efficiency monitoring was performed during 7–10 months of age. Body weight and feed intake were not significantly different between the transfaunated and unfaunated cattle. Transfaunation increased the relative levels of acetate and butyrate but decreased those of propionate, which increased the non‐glucogenic/glucogenic short‐chain fatty acid ratio. Microbial 16S, 18S, and ITS ribosomal RNA gene amplicon analysis showed that rumen microbial diversity and composition differed between transfaunated and unfaunated cattle; transfaunation increased the abundance of acetate‐ and butyrate‐producing bacteria, and decreased the abundance of bacterial genera associated with propionate production. Transfaunation also increased the abundance of Methanomassiliicoccaceae_group10 (1.94% vs. 0.05%) and Neocallimastix (27.1% vs. 6.8%) but decreased that of Methanomicrobium (<0.01% vs. 0.06%). Our findings indicate that rumen microbiota transfaunation shifts rumen fermentation toward acetate and butyrate production through a change in the rumen microbial composition in Japanese Black cattle.

Exploring the Rumen Microbiota and Serum Metabolite Profile of Hainan Black Goats with Different Body Weights before Weaning

“…Previous research, such as the work by Chiquette et al [38], demonstrated that the introduction of Prevotella bryantii 25A into the rumen of dairy cows via a rumen fistula led to increased butyrate levels. Similarly, Takizawa et al [39] found that microbiota translocation in Japanese black cattle resulted in a higher abundance of Prevotellaceae_UCG-004, subsequently elevating acetate and butyrate levels. This was further supported by Zhao et al [40], who confirmed through in vitro fermentation tests that Prevotellaceae_UCG-001 contributes to lignocellulose degradation and elevates VFA concentrations.…”

Assessing the impact of three feeding stages on rumen bacterial community and physiological characteristics of Japanese Black cattle

“…Multiple factors contribute to the variation in rumen microbiota, and breed per se significantly impacts the composition of cattle ruminal microbiota 9 – 11 . Recently, there has been ongoing analysis of the rumen microbiome in Japanese Black cattle 12 , 13 , but discussions have mainly focused on the relationship between rumen fermentation characteristics and microorganisms. Moreover, blood metabolites originate from the metabolites produced by rumen microbes; therefore, analyzing the connection between the rumen microorganisms influencing rumen fermentation and the blood metabolites reflecting the outcomes of rumen fermentation would be useful.…”