Effect of ruminal administration of the lactate-utilizing strain Megasphaera elsdenii (Me) NCIMB 41125 on abrupt or gradual transition from forage to concentrate diets

Henning, Pieter; Horn, C.H.; Leeuw, K-J.; Meissner, H.H.; Hagg, F.M.

doi:10.1016/j.anifeedsci.2010.02.002

Cited by 57 publications

(61 citation statements)

References 15 publications

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“…Counotte et al (11) found that more than 70% of lactic acid was fermented by M. elsdenii via the acrylate pathway in dairy cows and that propionic acid is the final product. Henning et al (17) reported that high-producing dairy cows treated with M. elsdenii after calving showed significantly increased VFA levels, particularly propionic acid, in the rumen. M. elsdenii altered the rumen fermentation patterns, which prompted the production of propionate.…”

Section: Discussionmentioning

confidence: 99%

Correlation between Composition of the Bacterial Community and Concentration of Volatile Fatty Acids in the Rumen during the Transition Period and Ketosis in Dairy Cows

Wang

Zhao

et al. 2012

Appl Environ Microbiol

107

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ABSTRACTThe transition period is a severe challenge to dairy cows. Glucose supply cannot meet demand and body fat is mobilized, potentially leading to negative energy balance (NEB), ketosis, or fatty liver. Propionate produces glucose by gluconeogenesis, which depends heavily on the number and species of microbes. In the present study, we analyzed the rumen microbiome composition of cows in the transition period, cows with ketosis, and nonperinatal cows by terminal restriction fragment length polymorphism (TRFLP) analysis of 16S rRNA genes and quantitative PCR. TRFLP analysis indicated that the quantity ofVeillonellaceaeorganisms was reduced and that ofStreptococcaceaeorganisms was increased in rumen samples from the transition period and ketosis groups, with the number ofLactobacillaceaeorganisms increased after calving. Quantitative PCR data suggested that the numbers of the main propionate-producing microbes,Megasphaera elsdeniiandSelenomonas ruminantium, were decreased, while numbers of the main lactate-producing bacterium,Streptococcus bovis, were increased in the rumen of cows from the transition period and ketosis groups, with the number ofLactobacillussp. organisms increased after calving. Volatile fatty acid (VFA) and glucose concentrations were decreased, but the lactic acid concentration was increased, in rumen samples from the transition period and ketosis groups. Our results indicate that the VFA concentration is significantly related to the numbers ofSelenomonas ruminantiumandMegasphaera elsdeniiorganisms in the rumen.

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Section: Discussionmentioning

confidence: 99%

Correlation between Composition of the Bacterial Community and Concentration of Volatile Fatty Acids in the Rumen during the Transition Period and Ketosis in Dairy Cows

Wang

Zhao

et al. 2012

Appl Environ Microbiol

107

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“…but in vitro (Counotte et al, 1981) and in vivo (Klieve et al, 2003) studies have shown that M. elsdenii is the key species performing this function. It has been shown that in cows supplemented with a M. elsdenii DFM, the pattern of rumen fermentation was altered in favour of propionate with potential benefits on energy balance and animal productivity (Henning et al, 2010;Aikman et al, 2011). Hino et al (1994) have also shown that combining lactate-producing bacteria with M. elsdenii is effective for increasing propionate production.…”

Section: Propionate-forming Bacteriamentioning

confidence: 99%

The use of direct-fed microbials for mitigation of ruminant methane emissions: a review

Jeyanathan

Martin

Morgavi

2014

Animal

132

115

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Concerns about the environmental effect and the economic burden of methane (CH 4 ) emissions from ruminants are driving the search for ways to mitigate rumen methanogenesis. The use of direct-fed microbials (DFM) is one possible option to decrease CH 4 emission from ruminants. Direct-fed microbials are already used in ruminants mainly to increase productivity and to improve health, and are readily accepted by producers and consumers alike. However, studies on the use of DFM as rumen CH 4 mitigants are scarce. A few studies using Saccharomyces cerevisiae have shown a CH 4 -decreasing effect but, to date, there has not been a systematic exploration of DFM as modulators of rumen methanogenesis. In this review, we explored biochemical pathways competing with methanogenesis that, potentially, could be modulated by the use of DFM. Pathways involving the redirection of H 2 away from methanogenesis and pathways producing less H 2 during feed fermentation are the preferred options. Propionate formation is an example of the latter option that in addition to decrease CH 4 formation increases the retention of energy from the diet. Homoacetogenesis is a pathway using H 2 to produce acetate, however up to now no acetogen has been shown to efficiently compete with methanogens in the rumen. Nitrate and sulphate reduction are pathways competing with methanogenesis, but the availability of these substances in the rumen is limited. Although there were studies using nitrate and sulphate as chemical additives, use of DFM for improving these processes and decrease the accumulation of toxic metabolites needs to be explored more. There are some other pathways such as methanotrophy and capnophily or modes of action such as inhibition of methanogens that theoretically could be provided by DFM and affect methanogenesis. We conclude that DFM is a promising alternative for rumen methane mitigation that should be further explored for their practical usage.

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“…The condition usually occurs during transition from an all-roughage to a high-concentrate diet over a short time when the microbial population and the rumen environment have not adapted properly to the fermentation medium of primarily starches and sugars. To prevent the condition, lactic acid needs to be removed rapidly, in which the robust strain NCIMB 41125 of the lactic acid utilizing species Megasphaera elsdenii excels (Henning et al, 2010a;Meissner et al, 2010). The strain (hereafter referred to as M.e.)…”

Section: ____________________________________________________________mentioning

confidence: 99%

“…Higher intakes with less animal variation and day-to-day variation during the initial adaptation phases when M.e. was administered have been reported in most experimental station trials (Henning et al, 2010a;b;Meissner et al, 2010), although there are exceptions (Leeuw et al, 2009), especially in commercial trials (Meissner et al, 2010). The reasons could be associated with rate of adaptation, dietary energy level and grain processing.…”

mentioning

confidence: 99%

Short communication: Megasphaera elsdenii on the performance of steers adapting to a high-concentrate diet, using three or five transition diets

Drouillard¹,

Henning²,

Meissner³

et al. 2012

SA J. An. Sci.

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________________________________________________________________________________ AbstractMegasphaera elsdenii (M.e.) NCIMB 41125 is a robust lactate utilizing strain of M.e. that is effective in minimizing the risk of ruminal acidosis in feedlot cattle. When dosed orally, cattle adapt smoothly to increasing concentrates in the diet, the incidence of digestive disturbances, morbidity and mortality is reduced, and carcass yield improves. One could therefore expect that the smooth transition should benefit overall performance. Dosing with the organism also provides the opportunity of a reduction in the time necessary for adaptation, rendering a further decrease in the cost of feeding. These two objectives were tested with 80 yearling crossbred steers blocked by weight before allotment to the respective treatments. The trial design was a randomized 2 × 2 factorial of two drench treatments (M.e. vs. placebo) and two adaptation periods (17 vs. 8 days). In the M.e. treatment, 40 steers were dosed orally on day 1 of the trial with 200 mL inoculum containing 10 11 cells. In the placebo treatment, the other 40 steers were dosed orally with only the 200 mL inoculum. In the 17-day transition period, five diets (5-transition) were used, which increased progressively in concentrate percentage, whereas in the 8-day transition period only three of the five diets were fed (3-transition). The steers were fed individually for 63 days before being transferred to group pens and fed until day 95, when they were slaughtered. Dry matter intake was not affected by dose or transition treatment. Body weight at 28 days and 63 days did not differ between dose and transition treatments; neither did ADG and FCR. Hot carcass weight was higher in M.e. steers than in placebo steers. None of the parameters differed significantly between the 5-transition and the 3-transition treatments. It was concluded that dosing with M.e. NCIMB 41125 should provide a small benefit to performance of feedlot cattle, with a further benefit in cost savings as dosing with the organism should allow a shorter adaptation period. ________________________________________________________________________________Keywords: Steers, transition diets, feed intake, body weight, growth, growth, carcass characteristics # Corresponding author: Kleeuw@arc.agric.zaRuminal acidosis in feedlot cattle manifests when lactic acid accumulates rapidly and pH declines as a result. The condition usually occurs during transition from an all-roughage to a high-concentrate diet over a short time when the microbial population and the rumen environment have not adapted properly to the fermentation medium of primarily starches and sugars. To prevent the condition, lactic acid needs to be removed rapidly, in which the robust strain NCIMB 41125 of the lactic acid utilizing species Megasphaera elsdenii excels (Henning et al., 2010a;Meissner et al., 2010). The strain (hereafter referred to as M.e.) is dosed at the commencement of the adaptation phase and supports a smooth transition as observed, with a...

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Effect of ruminal administration of the lactate-utilizing strain Megasphaera elsdenii (Me) NCIMB 41125 on abrupt or gradual transition from forage to concentrate diets

Cited by 57 publications

References 15 publications

Correlation between Composition of the Bacterial Community and Concentration of Volatile Fatty Acids in the Rumen during the Transition Period and Ketosis in Dairy Cows

Correlation between Composition of the Bacterial Community and Concentration of Volatile Fatty Acids in the Rumen during the Transition Period and Ketosis in Dairy Cows

The use of direct-fed microbials for mitigation of ruminant methane emissions: a review

Short communication: Megasphaera elsdenii on the performance of steers adapting to a high-concentrate diet, using three or five transition diets

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