Effect of Salicylic Acid, 2,4-D and 2i-P on the Production of Secondary Metabolites in Garcinia brasiliensis Mart. Callus

Teixeira, Maria Gessi; Carvalho, Marília; Leite, Marco Aurélio; Barbosa, Sandro; Filho, Plínio Rodrigues dos Santos; Santos, Breno Régis

doi:10.1590/1678-4324-2019170303

Cited by 5 publications

(2 citation statements)

References 43 publications

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“…& Arn [13]. Likewise, the significant effect of 2iP on TPs and TFs has been reported before in other ornamental and medicinal species such as Garcinia brasiliensis Mart [37]. Moreover, TFs were found to be high in the callus obtained from MS medium treated with Kin (2.0 mg/L) combined with NAA (1.0 mg/L) of Canscora decussata (Roxb.)…”

Section: Acclimatizationsupporting

confidence: 60%

Production of phenolics and flavonoids with antioxidant evaluations of Brunfelsia pauciflora (Cham. &Schltdl.) Benth. through in vitro propagated cultures.

Sayed

Marzouk²,

Sokkar

2022

Egypt. J. Chem.

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The present study considered the in vitro propagation of Brunfelsia pauciflora utilizing various cytokinins, as well the estimation of the antioxidant, total phenolics (TPs), and flavonoids (TFs) for shootlets of each treatment. The explants were sterilized with 0.2% mercuric chloride (20 min) and then cultured on Murashige and Skoog medium (MS) supplemented with three cytokinins; benzyl aminopurine (BAP), 6-Furfuryl-aminopurine (Kin), and isopentenyl adenine (2iP) at different concentrations. The highest shootlets number and fresh weight were obtained using 1.0 mg/L BAP, while shootlets length is increased using 2.0 mg/L kin. Additionally, significant numbers of leaves were observed for the plantlets supplemented with kin (1.0 & 3.0 mg/L) and 2iP (2.0 & 3.0 mg/L). The shootlets were rooted one month later with different strengths (full, ¾, ½, or ¼) on MS media without or with 1.0g/L activated charcoal (AC). Shootlet cultured on full-strength MS medium with AC gave the longest root, while the greatest numbers of roots were recorded using ¾ strength without AC. All the rooted shootlets were acclimatized on peat moss and recorded with the longest root length and greatest leaves and roots numbers. Alternatively, the methanolic extract of shootlets supplemented with 2iP (3.0 mg/L) yielded the maximum antioxidant effect (89.89 ± 0.28 mg Trolox /g extract), TPs (57.574±0.82 mg Gallic/g extract), and TFs (46.146 ± 0.31 mg Catechin/g extract) compared with the control (MS free) and mother plant. In conclusion, the antioxidant potential was shown to be strongly correlated with the TFs and the number of leaves /shootlet.

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Section: Acclimatizationsupporting

confidence: 60%

Production of phenolics and flavonoids with antioxidant evaluations of Brunfelsia pauciflora (Cham. &Schltdl.) Benth. through in vitro propagated cultures.

Sayed

Marzouk²,

Sokkar

2022

Egypt. J. Chem.

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“…However, according to [26] the highest level of phenolic compounds was found in the calluses of Leucojum aestivum initiated and multiplied on the medium supplemented with dicamba. According to [35] Phenols are secondary metabolic compounds present in vegetables and synthesized in the shikimic acid pathway. These compound present different chemical structures as well as different biological properties and can influence the texture of cellular structures.…”

Section: Discussionmentioning

confidence: 99%

Effect of auxin type on the total phenols synthesis and some enzymatic proteins activity in maize callus from seeds irradiated to gamma radiation

Ahou-Nadia¹,

Koutoua²,

Salomé³

et al. 2020

World J. Adv. Res. Rev.

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Secondary metabolites of plants widely used in the medicinal and cosmetic industries, can be produced by tissue culture. In vitro production of these secondary metabolites is affected by various factors such as the type and concentration of phyto regulators. In this study, 2,4-dichlorophenoxyacetic acid (2,4-D) and 2-methoxy-3,6-dichlorobenzoic acid (Dicamba) effect on phenolic compounds synthesis as well as Phenylalanine ammonia lyase (PAL), tyrosine ammonia lyase (TAL), Polyphenoloxidase (PPO), peroxidase (POD), Catalase (CAT) and Ascorbate Peroxidase (APX) activities from root explants have been studied. The root explants come from the seeds in vitro germination of maize irradiated (200 and 300 grays) or not irradiated (control) with gamma radiation. The results obtained showed that the auxin type had a very highly significant effect (p <0.001) on all parameters studied. Callus induced on medium containing 2,4-D gave a higher content of phenolic compounds, PAL, TAL, PPO, POD, CAT and APX than callus induced on medium containing dicamba. The highest content of total phenols (76.971 µmol/g fresh callus) was obtained in callus from 300 grays. By cons, the contents of POD, PAL, TAL, CAT and APX (4.034; 0.031; 0.145; 1315.596 and 2461.714 mmol/min/g fresh callus) were obtained in callus from 200 grays.

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In Vitro Production of Bioactive Compounds from Plant Cell Culture

Lakshmaiah¹,

Banadka²,

Thirumoorthy³

et al. 2022

Nutraceuticals Production From Plant Cell Factory

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Effect of Salicylic Acid, 2,4-D and 2i-P on the Production of Secondary Metabolites in Garcinia brasiliensis Mart. Callus

Cited by 5 publications

References 43 publications

Production of phenolics and flavonoids with antioxidant evaluations of Brunfelsia pauciflora (Cham. &Schltdl.) Benth. through in vitro propagated cultures.

Production of phenolics and flavonoids with antioxidant evaluations of Brunfelsia pauciflora (Cham. &Schltdl.) Benth. through in vitro propagated cultures.

Effect of auxin type on the total phenols synthesis and some enzymatic proteins activity in maize callus from seeds irradiated to gamma radiation

In Vitro Production of Bioactive Compounds from Plant Cell Culture

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