Effect of saline osmolarity on the steady-state level of water and electrolytes in kidney cortex cells

Kleinzeller, A.; Nedvı́dková, J; Knotková, A.

doi:10.1016/0005-2736(67)90122-8

Cited by 20 publications

(7 citation statements)

References 20 publications

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“…Like them, we could offer no adequate explanation for the ionic changes when examining the distribution of ions according to the Nernst equations (Table 4). It is clear from the present results, as well as those of Sigler and Janacek (1971) and Kleinzeller et al (1967) who also measured cellular potentials, that the observed ionic changes due to changes in osmolality cannot be explained simply by extrapolating from the normal condition at physiological Na concentrations and osmolalities.…”

Section: Discussionsupporting

confidence: 68%

“…Kleinzeller et al (1967) with a similar experimental arrangement showed no change in cell Na or K content but an increase in concentration of both. Our K data agree with theirs but Na data are opposite, again, possibly due to the temperature differences and nearly complete absence of bicarbonate from their solutions.…”

Section: Discussionmentioning

confidence: 75%

“…The experiments of Hughes and MacKnight (1976) may not be directly applicable to phenomena of kidney transport, since they were performed at 25° and because their solution lacked bicarbonate ion, shown to play a significant role in renal cellular Na transport (Fromter, Rumrich and Ullrich, 1973). There are no data in the literature on cellular content or concentration of Na, K or Cl in response to hypertonic saline and non-electrolyte solutions at 37° in kidney cortical cells, although Kleinzeller, Nedvidkova and Knotkova (1967) reported changes at 25° but with only low Na and mannitol.…”

Section: Introductionmentioning

confidence: 99%

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EFFECT OF HYPO‐ AND HYPERTONIC SALINE AND RAFFINOSE ON CELL CONTENT OF Na, Cl and K IN RESPIRING RAT KIDNEY CORTEX AT 37°

Kweifio-Okai

1981

Aust J Exp Biol Med

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Summary. Changes in cell Na, K and Cl content (per kg dry weight) and concentration (per kg intracellular water) were measured in respiring rat renal cortical slices incubated for 60 min at 37° in hypo-and hypertonic saline with and without the addition of a non-electrolyte (raffinose). Both hypertonic saline and raffinose increased cellular concentration of Na, Cl and K, the former by producing minimal cell shrinkage and major entry of Na and Cl into the cell, the latter entirely by cell shrinkage leading also to a loss of Na content but not of K. In hypotonic saline both content and concentration of Na and Cl did not change significantly, whereas that for K dropped markedly. Addition of ouabain (1 mmoI/1) produced a significant gain of cell Na and loss of K on a one to one basis but did not alter the effect of raffinose. It is concluded that sustained changes in cell ion concentrations and content in response to pericellular osmolality are produced and that these are directed towards equilibrating cellular activity of water to that of the surrounding medium.

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Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

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EFFECT OF HYPO‐ AND HYPERTONIC SALINE AND RAFFINOSE ON CELL CONTENT OF Na, Cl and K IN RESPIRING RAT KIDNEY CORTEX AT 37°

Kweifio-Okai

1981

Aust J Exp Biol Med

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“…This cannot be assessed by considering the intracellular compartment as homogeneous with respect to solute distribution. Compartmentalization of Cl occurs in renal cortical cells (Kleinzeller, Nedvidkova & Knotkova, 1967), and studies on washout of 36Cl suggest that this may also occur in medullary cells (Law, 1977).…”

Section: Discussionmentioning

confidence: 99%

The influence of chloride ions on renal outer medullary cell volume.

Law¹

1979

The Journal of Physiology

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SUMMARY1. Slices of rat renal outer medulla have been incubated in media made hyperosmotic (540 and 1055 m-osmole/kg H20) by the addition of urea, and containing variable concentrations of Cl (90, 144, 189 and 215 mM) and constant concentrations of Na (180 mM) and K (5.9 mM). A small number of incubations have been conducted in the presence of 100 mM-Na.2. Changes in cell volume during incubation have been calculated on the basis of initial and final slice weight and inulin space.3. The capacity of cells to shrink in response to extracellular osmotic stress was related principally to the external Cl concentration rather than to osmolality, increases in concentration being associated with enhanced shrinkage. Shrinkage was accompanied by net loss of cellular Cl. The ratio between intra-and extracellular C1 concentration (ca. 0.41) remained constant in all media.4. In media containing low Cl concentration (90 mM), reduction of media Na concentration to 100 mM enhanced shrinkage. This effect was not observed when medium Cl concentration was greater than 90 mM.5. Ethacrynic acid-cysteine (1 mM) significantly impaired the shrinkage response to extracellular osmotic stress, and caused cell swelling in media of relatively low Cl concentration and osmolality. It did not abolish the dependency of cell volume upon Cl concentration. There was marked reduction in the net amount of Cl lost from cells. 6. Ethacrynic acid-cysteine caused an increase in cellular Na content only in media containing 540 m-osmole/kg H20 and Cl concentrations less than 215 mm.7. Ouabain (1 mM) inhibited cell shrinkage to a lesser extent than ethacrynic acidcysteine in all media except that causing the greatest shrinkage under control conditions (215 mM-Cl/1055 m-osmole/kg H20). It is suggested that a ouabain-sensitive process may play an increasingly important role in Cl-related cell shrinkage as this becomes more pronounced.8. The findings are consistent with the view that Cl ions influence cell volume both through their effective external osmotic pressure and by means of Cl-specific process; the latter is associated with net loss of cellular Cl. A dependence of this loss upon Na/K exchange-linked metabolism is inferred, but the present findings do not permit the active or passive nature of the extrusion to be defined.

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“…Previous studies ofrenal volume regulation have used tissue slices, cell suspensions, isolated tubules, and single cells (1)(2)(3)(4)(5)(6)(7)(8)(22)(23)(24)(25)(26)(27)(28)(29)(30). In all of these studies the medium osmolality was changed suddenly, which is uncharacteristic of osmotic changes that might be encountered in pathophysiologic states in vivo.…”

Section: Introductionmentioning

confidence: 99%

Isovolumetric regulation of isolated S2 proximal tubules in anisotonic media.

Lohr¹,

Grantham²

1986

J. Clin. Invest.

124

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Sudden alteration in medium osmolality causes an osmometric change in proximal tubule cell size followed by restoration of cell volume toward normal in hypotonic but not in hypertonic medium. We determined the capability of isolated nonperfused proximal tubules to prevent a change in cell volume in anisotonic media. The external osmolality was gradually changed over a range from 110 to 480 mosM. At 1.5 mosM/min, cell volume remained constant between 167±9 and 361±7 mosM, a phenomenon termed isovolumetric regulation (IVR). Cells lost intracellular solutes in hypotonic and gained intracellular solutes in hypertonic media. Raffinose or choline chloride substitution showed that osmolality, rather than NaCG, signalled cell volume maintenance in hyperosmotic media. Cooling (7-100C) blocked IVR. IVR was maintained when osmolality was lowered at a rate of 27, but not at 42 mosM/min. IVR was not observed when the rate of osmolality increase exceeded 3 mosM/min. We conclude that proximal tubule cells sensitively regulate intracellular volume in an osmolality range of pathophysiologic interest by mechanisms dependent on (a) the rate of net water movement across basolateral membranes and (b) the absolute intracellular content of critical solutes.

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Effect of saline osmolarity on the steady-state level of water and electrolytes in kidney cortex cells

Cited by 20 publications

References 20 publications

EFFECT OF HYPO‐ AND HYPERTONIC SALINE AND RAFFINOSE ON CELL CONTENT OF Na, Cl and K IN RESPIRING RAT KIDNEY CORTEX AT 37°

EFFECT OF HYPO‐ AND HYPERTONIC SALINE AND RAFFINOSE ON CELL CONTENT OF Na, Cl and K IN RESPIRING RAT KIDNEY CORTEX AT 37°

The influence of chloride ions on renal outer medullary cell volume.

Isovolumetric regulation of isolated S2 proximal tubules in anisotonic media.

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