Effect of salinity and pH on growth, phycoerythrin, and non-volatile umami taste active compound concentration of Rhodomonas salina using a D-optimal design approach

Latsos, Christos; Bakratsas, Georgios; Moerdijk, Tanja; Houcke, Jasper van; Timmermans, Klaas R.

doi:10.1007/s10811-021-02547-4

Cited by 15 publications

(12 citation statements)

References 79 publications

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“…Phycoerythrin constitutes 12 to 20% of the total protein content (Seixas et al 2009;Barka and Blecker 2016). The increased phycoerythrin concentration at pH 8.5 is in line with previous literature on R. salina (Latsos et al 2021a). This increase of phycoerythrin can be associated with the antioxidant activity of the pigment as a response to the ROS increase at alkaline conditions (Samarakoon and Jeon 2012;Jung et al 2016;Gauthier et al 2020).…”

Section: Cellular Compositionsupporting

confidence: 91%

“…The productivity of R. salina was higher at pH 7 in comparison with pH 8.5. This result is analogous to the literature, in which many studies have been reported pH 7 as the optimal for the growth and the production rate of Rhodomonas (Berge et al 2010;Latsos et al 2021a). At pH 8.5, the productivity was lower, while in high light intensities (> 35 mol photons m −2 day −1 ), the biomass in the reactor collapsed.…”

Section: Productivitysupporting

confidence: 90%

“…This study investigated the influence of pH in the growth, biochemical composition, and taste of R. salina which was successfully cultivated on a semi-large scale under natural sunlight conditions with productivity varying from 0.034 to 0.137 g L −1 day −1 . The highest productivity that has been reported in continuous culture for R. salina is 1.17 g L −1 day −1 (Latsos et al 2021a). However, it is noteworthy that this high productivity was obtained in a small scale system (0.4 L) and under constant artificial light conditions (Oostlander et al 2020a;Thoisen et al 2020).…”

Section: Productivitymentioning

confidence: 95%

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Effect of pH on Rhodomonas salina growth, biochemical composition, and taste, produced in semi-large scale under sunlight conditions

et al. 2022

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Rhodomonas salina is a microalgal species, belonging to the cryptophytes, and is widely used as aquaculture feed because of its high nutritional profile and phycoerythrin content. This study investigated the effect of pH on the growth, biochemical composition, and taste of R. salina when cultivated on a semi-large scale under natural light conditions. Two tubular photobioreactors (200 L) were used for the cultivation of R. salina with sunlight as the only illumination source. Two different pH setpoints were applied, 7 and 8.5. Optimal temperature and nutrient conditions were applied, according to previous research findings. The results demonstrated that the productivity of R. salina was higher at pH 7, 0.06–0.14 gdry weight L−1 day−1, compared to pH 8.5, 0.03–0.12 gdry weight L−1 day−1. It was found that protein and total fatty acid concentrations were higher in the biomass that was produced at pH 8.5, 33.7% and 12.3% of dry weight, respectively, while at pH 7, the protein content was 31.9% and the total fatty acids 8.8% of dry weight. The phycoerythrin concentration, like protein, was higher at pH 8.5, 2.7% of dry weight, compared to pH 7, 1% of dry weight. The free amino acid and nucleotide profile of R. salina was affected by the pH, resulting in increased equivalent umami concentration at pH 7. For the sensory evaluation, an expert panel on algae flavors evaluated the effect of pH on the taste of R. salina, reporting that the biomass that was produced at pH 7 had more umami flavor than the biomass that was produced at pH 8.5, which was evaluated as more bitter.

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Section: Cellular Compositionsupporting

confidence: 91%

Section: Productivitysupporting

confidence: 90%

Section: Productivitymentioning

confidence: 95%

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Effect of pH on Rhodomonas salina growth, biochemical composition, and taste, produced in semi-large scale under sunlight conditions

et al. 2022

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“…It has been shown that the amount of PE accumulating in Rhodomonas sp. depends on cultivation conditions [ 24 , 45 ]. Thus, an experimental screening of the factors that could impact the production of PE was performed using a Plackett–Burman design.…”

Section: Discussionmentioning

confidence: 99%

Improvement of Biomass and Phycoerythrin Production by a Strain of Rhodomonas sp. Isolated from the Tunisian Coast of Sidi Mansour

et al. 2022

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Microalgae are photoautotrophic microorganisms known as producers of a large variety of metabolites. The taxonomic diversity of these microorganisms has been poorly explored. In this study, a newly isolated strain was identified based on the 18S rRNA encoding gene. The phylogenetic analysis showed that the isolated strain was affiliated with the Rhodomonas genus. This genus has greatly attracted scientific attention according to its capacity to produce a large variety of metabolites, including phycoerythrin. Growth and phycoerythrin production conditions were optimized using a Plackett–Burman design and response surface methodology. An expression profile analysis of the cpeB gene, encoding the beta subunit of phycoerythrin, was performed by qRT-PCR under standard and optimized culture conditions. The optimization process showed that maximum cell abundance was achieved under the following conditions: CaCl2 = 2.1328 g/L, metal solution = 1 mL/L, pH = 7 and light intensity = 145 μmol photons/m2/s, whereas maximum phycoerythrin production level occurred when CaCl2 = 1.8467 g/L, metal solution = 1 mL/L, pH = 7 and light intensity = 157 μmol/m2/s. In agreement, positive transcriptional regulation of the cpeB gene was demonstrated using qRT-PCR. This study showed the successful optimization of abiotic conditions for highest growth and phycoerythrin production, making Rhodomonas sp. suitable for several biotechnological applications.

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“…Nevertheless, even the highest EUC reached in D. salina DF15 under red light (3.2 g MSG/100 g AFDW) is still considerably lower than for other microalgae and foods such as meat and mushroom. The highest EUC found in Rhodomonas salina was reported to be 23.4 g MSG/100 g AFDW when cultivated at pH 8.5 and 30% salinity [31]. In Sousvide beef, the EUC can rise as high as 53.3 g MSG/100 g AFDW [32].…”

Section: Protein and Amino Acid Dynamicsmentioning

confidence: 99%

A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths

Sui

Mazzucchi

Acharya

et al. 2021

Foods

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Strains of Dunaliella salina microalgae are of considerable research and industrial interest because they hyper-accumulate β-carotene as well as produce high-quality protein. To explore the co-production of valuable compounds in D. salina, this study compared the production of β-carotene, phytoene and amino acids in two strains cultivated under white, red or blue light until no further nitrogen was available. D. salina DF15 (CCAP 19/41 (PLY DF15)) produced more than 12% β-carotene (ash-free dry weight (AFDW) basis), and red light triggered the production of 9-cis β-carotene at a 9-cis/all-trans β-carotene ratio of 1.5. Phytoene production was also evident in D. salina DF15 under all conditions, particularly under blue light. However, the profile of essential amino acids (EAAs) and calculation of the essential amino acid index (EAAI) was less than ideal in terms of protein quality, for both strains. Umami compounds, quantified as monosodium glutamate (MSG) equivalents, indicated a higher equivalent umami concentration (EUC) in D. salina DF15 under red light (3.2 g MSG/100 g AFDW) than in D. salina CCAP19/30. Overall, D. salina DF15 demonstrates valuable traits for further exploration and product optimisation.

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Effect of salinity and pH on growth, phycoerythrin, and non-volatile umami taste active compound concentration of Rhodomonas salina using a D-optimal design approach

Cited by 15 publications

References 79 publications

Effect of pH on Rhodomonas salina growth, biochemical composition, and taste, produced in semi-large scale under sunlight conditions

Effect of pH on Rhodomonas salina growth, biochemical composition, and taste, produced in semi-large scale under sunlight conditions

Improvement of Biomass and Phycoerythrin Production by a Strain of Rhodomonas sp. Isolated from the Tunisian Coast of Sidi Mansour

A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths

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