Effect of salinity on chlorophyll and proline contents in three aquatic macrophytes

Rout, N.P.; Tripathi, Shashi Bhushan; Shaw, Birendra Prasad

doi:10.1023/a:1001186502386

Cited by 22 publications

(8 citation statements)

References 23 publications

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“…Pumpkin ( Cucurbita pepo ) and ground nut ( Vigna subterranea ) root and shoot length, FW and DW decreased in correlation to increasing salinity ( Ambede et al, 2012 ; Kurum et al, 2013 ). Moreover, a decrease in chlorophyll content and photosynthetic efficiency under salinity stresses was observed in various plant species ( Rout et al, 1997 ; Jamil et al, 2007 ; Ambede et al, 2012 ). Na + ions accumulated in the roots of salt tolerant as well as salt sensitive green bean seedlings upon exposure to salinity stress.…”

Section: Discussionmentioning

confidence: 99%

High Salinity Induces Different Oxidative Stress and Antioxidant Responses in Maize Seedlings Organs

et al. 2016

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Salinity negatively affects plant growth and causes significant crop yield losses world-wide. Maize is an economically important cereal crop affected by high salinity. In this study, maize seedlings were subjected to 75 mM and 150 mM NaCl, to emulate high soil salinity. Roots, mature leaves (basal leaf-pair 1,2) and young leaves (distal leaf-pair 3,4) were harvested after 3 weeks of sowing. Roots showed the highest reduction in biomass, followed by mature and young leaves in the salt-stressed plants. Concomitant with the pattern of growth reduction, roots accumulated the highest levels of Na+ followed by mature and young leaves. High salinity induced oxidative stress in the roots and mature leaves, but to a lesser extent in younger leaves. The younger leaves showed increased electrolyte leakage (EL), malondialdehyde (MDA), and hydrogen peroxide (H2O2) concentrations only at 150 mM NaCl. Total antioxidant capacity (TAC) and polyphenol content increased with the increase in salinity levels in roots and mature leaves, but showed no changes in the young leaves. Under salinity stress, reduced ascorbate (ASC) and glutathione (GSH) content increased in roots, while total tocopherol levels increased specifically in the shoot tissues. Similarly, redox changes estimated by the ratio of redox couples (ASC/total ascorbate and GSH/total glutathione) showed significant decreases in the roots. Activities of enzymatic antioxidants, catalase (CAT, EC 1.11.1.6) and dehydroascorbate reductase (DHAR, EC 1.8.5.1), increased in all organs of salt-treated plants, while superoxide dismutase (SOD, EC 1.15.1.1), ascorbate peroxidase (APX, EC 1.11.1.11), glutathione-s-transferase (GST, EC 2.5.1.18) and glutathione reductase (GR, EC 1.6.4.2) increased specifically in the roots. Overall, these results suggest that Na+ is retained and detoxified mainly in roots, and less stress impact is observed in mature and younger leaves. This study also indicates a possible role of ROS in the systemic signaling from roots to leaves, allowing leaves to activate their defense mechanisms for better protection against salt stress.

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Section: Discussionmentioning

confidence: 99%

High Salinity Induces Different Oxidative Stress and Antioxidant Responses in Maize Seedlings Organs

et al. 2016

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“…For chlorophyll extraction, the three-leaf sample for each treatment was homogenized in 95% ethanol. The homogenate was centrifuged at 10,000 × g and the supernatant was transferred out for determination of the absorbance values in DU730 Beckman spectrophotometer at 663 and 645 nm, respectively (Rout et al, 1998). The resulting absorbance values A 663 and A 645 were used to calculate the content of chlorophyll a (Chl a), chlorophyll b (Chl b) and the total chlorophyll (Chl T) using the following formula:…”

Section: Methodsmentioning

confidence: 99%

Reveromycins A and B from Streptomyces sp. 3–10: Antifungal Activity against Plant Pathogenic Fungi In vitro and in a Strawberry Food Model System

et al. 2017

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This study was conducted to determine the antifungal activity of the metabolites from Streptomyces sp. 3–10, and to purify and identify the metabolites. Meanwhile, the taxonomic status of strain 3–10 was re-evaluated. The cultural filtrates of strain 3–10 in potato dextrose broth were extracted with ethyl acetate. The resulting crude extract at 1 and 5 μg/ml inhibited growth of 22 species in 18 genera of plant pathogenic fungi and Oomycetes, accounting for 92% of the total 24 tested species, suggesting that it has a wide antifungal spectrum. Two compounds were purified from the crude extract and were identified as reveromycins A and B, which demonstrated high antifungal activity against Botrytis cinerea, Mucor hiemails, Rhizopus stolonifer, and Sclerotinia sclerotiorum under acidic pH conditions. Both the crude extract and reveromycin A from strain 3–10 at 10, 50, and 100 μg/ml showed high efficacy in suppression of strawberry fruit rot caused by the above-mentioned four pathogens. The efficacy was comparable to that of corresponding commercial fungicides (pyrimethanil, captan, dimetachlone) used in management of these pathogens. Morphological, physiological, and phylogenetic characterization showed that strain 3–10 is closely related to Streptomyces yanglinensis 1307T, representing a novel phylotype in that species. This study reported a new strain with reveromycins-producing capability. The finding is important for further exploitation of reveromycins for agricultural use.

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“…Another major effect was an increase in chlorophyll content, which exhibited an average increase of 114%. The higher levels of chlorophyll in transgenic plants subjected to salt stress would contribute to their ability to sustain photosynthesis and assimilation during episodes of salt stress ( Smillie and Nott, 1982 ; Rout et al, 1997 ). The overexpression of CIPK genes in transgenic plants also resulted in a 77% increase in seed germination when the transgenic plants were subjected to salt stress, a reliable indicator of salt tolerance ( Tahal et al, 2000 ; Shabala and Cuin, 2008 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of Important Physiological Traits and Moderators That Are Associated with Improved Salt Tolerance in CBL and CIPK Overexpressors through a Meta-Analysis

Cheng

et al. 2017

Front. Plant Sci.

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The CBL-CIPK pathway is a plant-specific Ca2+ sensor relaying pathway that has been shown to be involved in plant response to salt stress. Over-expression of CBL-CIPK network genes has been reported to increase salt tolerance in many studies. The studies on the overexpression of CBL-CIPK network genes, however, have used various indices to evaluate the effect of these genes on salt tolerance and have indicated a variety of roles for the major CBL-CIPK pathway genes. Therefore, it is of great interest to analyze the various effects resulting from the overexpression CBL-CIPK pathway genes and their relation to salt tolerance. The meta-analysis conducted in the present study investigated how over-expression of CBLs or CIPKs in transgenic plants affects the response to salt stress and identified the increase or decrease that occurs in these experimental variables when foreign CIPK or CBL genes are overexpressed in transgenic plants. The data from the collective studies on over-expression of CIPKs indicated that 6 of the 11 examined parameters (main effects) increased by 22% or more, while two of the six examined parameters increased by at least 78% in transgenic plants overexpressing CBL genes. In addition to analyzing the impact of overexpression on the main effects, eight different modifying parameters were also analyzed. Results indicated that several moderators impact the extent to which overexpression of CBLs and CIPKs affect the main parameters. The majority of CBLs have been obtained from dicotyledonous plants and most of the CBLs and CIPKs have been expressed in dicotyledonous plants. In comparison to homologous expression, the meta-analysis indicated that heterogeneous expression of CBLs resulted in greater increases in seed germination. The results of the meta-analysis provide information that could be useful in designing research to examine the mechanisms by which CBL-CIPK pathway genes increase salt tolerance in plants.

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Effect of salinity on chlorophyll and proline contents in three aquatic macrophytes

Cited by 22 publications

References 23 publications

High Salinity Induces Different Oxidative Stress and Antioxidant Responses in Maize Seedlings Organs

High Salinity Induces Different Oxidative Stress and Antioxidant Responses in Maize Seedlings Organs

Reveromycins A and B from Streptomyces sp. 3–10: Antifungal Activity against Plant Pathogenic Fungi In vitro and in a Strawberry Food Model System

Identification of Important Physiological Traits and Moderators That Are Associated with Improved Salt Tolerance in CBL and CIPK Overexpressors through a Meta-Analysis

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