Effect of salinity on growth of green alga Botryococcus braunii and its constituents

Ambati, Ranga Rao; Dayananda, C.; Ravi, Saranya; Shamala, T. R.; Ravishankar, G. A.

doi:10.1016/j.biortech.2006.02.007

Cited by 374 publications

(204 citation statements)

References 14 publications

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“…Similar effects were also observed in Chlamydomonas mexicana and Scenedesmus obliquus (Salama et al, 2013;Mohan and Devi, 2014). In contrast, the biomass yield was increased in the salinity stress, i.e., 17-34 mM in green microalgae, Botryococcus braunii, was also reported (Rao et al, 2007).…”

Section: Detection Of Mldp During Abiotic Stress-immunostainingsupporting

confidence: 70%

“…During salt stress condition, pronounced variation in pH, TDS, salinity, and conductivity were observed in Scenedesmus (Supplementary Table 1). According to Rao et al (2007), the pH of the algal cells varies with respect to salt concentration in green microalgae B. braunii.…”

Section: Effect Of Salt Stress On Biomass Of S Quadricaudamentioning

confidence: 99%

“…Abundant availability of seawater can be used for algal cultivation with biofuel application. According to Rao et al (2007), the presence of monounsaturated and polyunsaturated fatty acids was detected in B. braunii during salt stress with palmitoleic and oleic acids as major fatty acids.…”

Section: Effect Of Salt Stress On Biomass Of S Quadricaudamentioning

confidence: 99%

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Major Lipid Body Protein: A Conserved Structural Component of Lipid Body Accumulated during Abiotic Stress in S. quadricauda CASA-CC202

et al. 2016

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Abiotic stress in oleaginous microalgae enhances lipid accumulation, which is stored in a specialized organelle called lipid droplets (LDs). Both the LDs or lipid body are enriched with major lipid droplet protein (MLDP). It serves as a major structural component and also plays a key role in recruiting other proteins and enzymes involved in lipid body maturation. In the present study, the presence of MLDP was detected in two abiotic stress condition namely nitrogen starvation and salt stress condition. Previous research reveals that nitrogen starvation enhances lipid accumulation. Therefore, the effect of salt on growth, biomass yield, and fatty acid profile is studied in detail. The specific growth rate of Scenedesmus quadricauda under the salt stress of 10mM concentration is about 0.174 μ and in control, the SGR is 0.241 μ. An increase in the doubling time of the cells shows that the rate of cell division decreases during salt stress (2.87-5.17). The dry biomass content also decreased drastically at 50mM salt-treated cells (129 mg/L) compared to control (236 mg/L) on the day 20. The analysis of fatty acid composition also revealed that there is a 20% decrease in the saturated fatty acid level and 19.9% increment in monounsaturated fatty acid level, which makes salt-mediated lipid accumulation as a suitable biodiesel precursor.

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Section: Detection Of Mldp During Abiotic Stress-immunostainingsupporting

confidence: 70%

Section: Effect Of Salt Stress On Biomass Of S Quadricaudamentioning

confidence: 99%

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Major Lipid Body Protein: A Conserved Structural Component of Lipid Body Accumulated during Abiotic Stress in S. quadricauda CASA-CC202

et al. 2016

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“…In another study with Dunaliella tertiolecta, an increase in intracellular lipids (60% to 67%) and triglyceride concentration (40% to 56%) with an increase in NaCl concentration from 0.5 (freshwater concentration) to 1.0 M was observed [146]. Increasing the NaCl level in cultures of Botryococcus braunii, a fresh water alga, showed an increase in growth rate, carbohydrate content, and lipid content; however, the greatest biomass concentration was achieved at the lowest salinity level [147]. These results are supported by another study in which lipid content of Botryococcus braunii grown in 0.50 M NaCl was higher compared to media without NaCl addition, but protein, carbohydrates, and pigments levels were lower [143].…”

Section: Salinitymentioning

confidence: 93%

Effects of Environmental Factors and Nutrient Availability on the Biochemical Composition of Algae for Biofuels Production: A Review

2013

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Due to significant lipid and carbohydrate production as well as other useful properties such as high production of useful biomolecular substrates (e.g., lipids) and the ability to grow using non-potable water sources, algae are being explored as a potential high-yield feedstock for biofuels production. In both natural and engineered systems, algae can be exposed to a variety of environmental conditions that affect growth rate and cellular composition. With respect to the latter, the amount of carbon fixed in lipids and carbohydrates (e.g., starch) is highly influenced by environmental factors and nutrient availability. Understanding synergistic interactions between multiple environmental variables and nutritional factors is required to develop sustainable high productivity bioalgae systems, which are essential for commercial biofuel production. This article reviews the effects of environmental factors (i.e., temperature, light and pH) and nutrient availability (e.g., carbon, nitrogen, phosphorus, potassium, and trace metals) as well as cross-interactions on the biochemical composition of algae with a special focus on carbon fixation and partitioning of carbon from a biofuels perspective.

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“…The B. braunii strain UTEX LB 572 was kept active by weekly culture in 100ml Erlenmeyer flasks containing 50ml of BG11 modified medium (Rao et al 2007). B. braunii cultures were performed for 16 days in the same medium; its conditions were 200 rpm orbital shaking, 17.6 µmol/ m 2 s intensity of light for 24 hours, room temperature of 20 °C and daily blowing by 60 seconds with flows of 70 ml/ min of CO 2 to achieve 20% saturation in the atmosphere; which were the control culture conditions.…”

Section: Methodsmentioning

confidence: 99%

GROWTH PROMOTER EFFECT ON THE B. braunii KUTZING 1849 CULTURE BY SEVERAL DIFFERENT BACTERIAL STRAINS

et al. 2017

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There are several strategies to improve the growth of microalgae in industrial processes. In recent years, one of them has gained strength to achieve this goal: the co-culture with bacteria. Using growth-promoting substances, producer bacteria enhance microalgae biology activity, similar to how they have been used to promote the successful production of crops. The aim of this study was to evaluate the promoter capacity of strains Bacillus subtilis, Corynebacterium aquatile and Flavobacterium aquatile, evaluate their ability to improve the growth rate of microalgae Botryococcus braunii and to optimize the process derived from its cultivation. This study showed that the tested bacteria were able to increase up to 1.7 times the B. braunii growth rate and this promoting ability remained present in cell lysate preparations from the same bacterial strains. Key words: Botryococcus braunii, Flavobacterium aquatile, Corynobacterium aquatile, Bacillus subtilis, microbial elicitation, co-cultivation ResumenEl empleo de bacterias productoras de sustancias promotoras de crecimiento, para mejorar la eficiencia en el crecimiento de las microalgas y así potenciar su actividad en procesos industriales es una práctica que ha tomado fuerza durante los últimos años, de manera análoga a como han sido utilizadas las bacterias para favorecer la producción exitosa de cultivos vegetales. El objetivo de este trabajo fue evaluar la capacidad de las cepas Flavobacterium aquatile, Corynobacterium aquatile y Bacillus subtilis de actuar como promotoras en el crecimiento de la microalga Botryococcus braunii con el fin de mejorar su velocidad de crecimiento y optimizar los procesos derivados de su cultivo. Este estudio muestra que las bacterias evaluadas tienen la capacidad de aumentar hasta 1,7 veces el crecimiento de B. braunii y esta capacidad promotora continúa presente en preparaciones de lisados celulares procedentes de estas mismas cepas bacterianas.

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Effect of salinity on growth of green alga Botryococcus braunii and its constituents

Cited by 374 publications

References 14 publications

Major Lipid Body Protein: A Conserved Structural Component of Lipid Body Accumulated during Abiotic Stress in S. quadricauda CASA-CC202

Major Lipid Body Protein: A Conserved Structural Component of Lipid Body Accumulated during Abiotic Stress in S. quadricauda CASA-CC202

Effects of Environmental Factors and Nutrient Availability on the Biochemical Composition of Algae for Biofuels Production: A Review

GROWTH PROMOTER EFFECT ON THE B. braunii KUTZING 1849 CULTURE BY SEVERAL DIFFERENT BACTERIAL STRAINS

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