Effect of saliva and serum on the adherence of Candida species to chlorhexidine-treated denture acrylic

McCourtie, Jane; MacFarlane, T. W.; Samaranayake, LP

doi:10.1099/00222615-21-3-209

Cited by 49 publications

(45 citation statements)

References 15 publications

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“…A simple environment was preferred because the aim was to evaluate this virulence factor independently from the host factors. Moreover, several studies have been performed to examine the adhesion of C. albicans on acrylic surfaces or epithelial cells in the presence of bacteria, saliva and serum or carbohydrates 5,6,14,39,40…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, chemical cleaning with immersion denture cleaners is suggested as the first choice for plaque control in these patients 11,12. Candidal infections have been successfully treated with chlorhexidine gluconate because it is an effective oral disinfectant 13,14. Fittydent is a chemical cleaning agent that is also used to remove denture plaque 9…”

Section: Introductionmentioning

confidence: 99%

“…However, many of them are in vitro studies, which have proposed complex and difficult techniques. Researchers have problems in quantifying the candidal adherence to surfaces due to the co-aggregation of adhered yeasts and to the laborious, time-consuming removal methods 3,5,14. The aim of this study was to examine the adherence of Candida on the surfaces of acrylic denture prosthetic materials and the palatal mucosa in a group of patients and examine the in vitro adhesion abilities of these yeasts to acrylic strips.…”

Section: Introductionmentioning

confidence: 99%

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Effectiveness of Different Cleaning Agents against the Colonization of Candida spp and the in Vitro Detection of the Adherence of These Yeast Cells to Denture Acrylic Surfaces

et al. 2008

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PurposeThe aim of this study is to examine the effect Klorhex and Fittydent, which are used as cleaning agents on the adhesion of Candida on the surfaces of acrylic denture and palatal mucosa. In addition, ability of yeasts to adhere to acrylic strips was evaluated after applying these agents in vitro.Materials and MethodsEach group of 15 patients cleaned their dentures with either Klorhex or with Fittydent. The control group cleaned their dentures with water.ResultsIt was found that 62.2% of the patients had colonies of Candida species on their palatal mucosa which was reduced to 51.1% after using these cleaning agents. The colonization rate with Candida spp on their dentures was reduces from 82.2% to 68.8% using these cleaning agents. The mean adhesion value of the Candida strains isolated from the acrylic strips were found to be 75 cell/strip prior to applying the Klorhex and Fittydent and 37.5 cell/strip and 15 cell/strip after applying these agents, respectively.ConclusionThese results showed that Klorhex and Fittydent have a certain preventive effect on the colonization rate of Candida spp on the surface of these dentures, the palatal mucosa, as well as on the acrylic strips in vitro.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effectiveness of Different Cleaning Agents against the Colonization of Candida spp and the in Vitro Detection of the Adherence of These Yeast Cells to Denture Acrylic Surfaces

et al. 2008

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“…Nystatin, amphotericin B, 5fluorocytosine, clotrimazole, and ketoconazole significantly inhibited the adherence of C. albicans to BECs (55). Pretreatment of denture acrylic with chlorhexidine (48,56) and exposure of Candzda to sublethal concentrations of chlorhexidine (56) resulted in reduced yeast adhesion to denture material.…”

Section: Antibacterial Drugsmentioning

confidence: 99%

“…Extracted cells lacked this moiety but still possessed enough mannan for concanavalin A recognition and agglutination. 48 (iwn) free energy of the denture material may also influence Candida adhesion (6).…”

Section: Mannanmentioning

confidence: 99%

Oral adhesion of yeasts

Olsen

1990

Acta Odontologica Scandinavica

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Oral adhesion of yeasts probably occurs by interaction between yeast cell adhesins and oral epithelial cell receptors. In Candida albicans mannoprotein, glucan, chitin, cell wall proteins, and lipids are possible adhesins. Mannoprotein appears as a fibrillar or floccular outermost layer in stationary-phase cells grown in sugar-rich medium. Preincubation of buccal epithelial cells (BECs) with concanavalin A inhibits adhesion, as does suppression of mannoprotein production by tunicamycin. Germ tubes adhere more easily to BECs and plastic than do blastospores. Methyl-alpha-D-mannoside may be analogous to the yeast adhesin or epithelial cell receptor because it inhibits adhesion of C. albicans to BECs. L-Fucose, N-acetyl-D-glucosamine, or D-mannose, having the same effect, may also function as epithelial cell receptors. Other factors affecting yeast adhesion may be fibronectin, hydrophobicity, s-IgA, and indigenous bacteria. Growth of yeasts to stationary phase in sugar-rich media promotes adhesion to acrylic, as do divalent cations and serum. Saliva, chlorhexidine, and Streptococcus salivarius inhibit adhesion of yeasts.

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Levorotatory carbohydrates and xylitol subdue Streptococcus mutans and Candida albicans adhesion and biofilm formation

Brambilla

Ionescu

Cazzaniga

et al. 2015

J. Basic Microbiol.

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Dietary carbohydrates and polyols affect the microbial colonization of oral surfaces by modulating adhesion and biofilm formation. The aim of this study was to evaluate the influence of a select group of l-carbohydrates and polyols on either Streptococcus mutans or Candida albicans adhesion and biofilm formation in vitro. S. mutans or C. albicans suspensions were inoculated on polystyrene substrata in the presence of Tryptic soy broth containing 5% of the following compounds: d-glucose, d-mannose, l-glucose, l-mannose, d- and l-glucose (raceme), d- and l-mannose (raceme), l-glucose and l-mannose, sorbitol, mannitol, and xylitol. Microbial adhesion (2 h) and biofilm formation (24 h) were evaluated using MTT-test and Scanning Electron Microscopy (SEM). Xylitol and l-carbohydrates induced the lowest adhesion and biofilm formation in both the tested species, while sorbitol and mannitol did not promote C. albicans biofilm formation. Higher adhesion and biofilm formation was noted in both organisms in the presence of d-carbohydrates relative to their l-carbohydrate counterparts. These results elucidate, hitherto undescribed, interactions of the individually tested strains with l- and d-carbohydrates, and how they impact fungal and bacterial colonization. In translational terms, our data raise the possibility of using l-form of carbohydrates and xylitol for dietary control of oral plaque biofilms.

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Effect of saliva and serum on the adherence of Candida species to chlorhexidine-treated denture acrylic

Cited by 49 publications

References 15 publications

Effectiveness of Different Cleaning Agents against the Colonization of Candida spp and the in Vitro Detection of the Adherence of These Yeast Cells to Denture Acrylic Surfaces

Effectiveness of Different Cleaning Agents against the Colonization of Candida spp and the in Vitro Detection of the Adherence of These Yeast Cells to Denture Acrylic Surfaces

Oral adhesion of yeasts

Levorotatory carbohydrates and xylitol subdue Streptococcus mutans and Candida albicans adhesion and biofilm formation

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