In the 21 century, environmental pollution has been Background: acknowledged as one of the major problems. The textile and dyeing industries contribute a major portion by discharging intensely complex effluent consisting of highly noxious azoic dyes.In this study, biological treatment using acclimatized microorganisms Methods: were employed in search of a cheap and eco-friendly substitute for color removal from textile waste. The microbial inocula were isolated from effluent soil samples and then applied to flasks containing azo dyes as the only source of carbon for decolorization.Biochemical tests postulated predominance of and Results: Enterococcus bacterial strains. CO isolate or emerged as the best Bacillus Bacillus farraginis decolorizer of Orange M2R dye, decolorizing 98% of the dye. BG isolate or showed maximum decolorization on Green GS dye Paenibacillus macerans that decolorized 97% of the dye. The optimum physiochemical condition for decolorization of OM2R and GGS dye was pH 7.0, 2% NaCl conc., 1% initial dye conc. and 37°C temperature by the selected isolates.The findings were validated and have the potential for Conclusions: bioremediation in textile waste effluent treatment plants.