Effect of salt stress on nitrogen fixation by different cultivars of green beans

Pessarakli, Mohammad; Zhou, M.

doi:10.1080/01904169009364104

Cited by 11 publications

(4 citation statements)

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“…Salt tolerance of symbiotic nitrogen fixation reportedly depends both on the plant and on Rhizobium genotypes (Pessarakli and Zhou 1990; Cordovilla et al . 1995).…”

Section: Introductionmentioning

confidence: 99%

“…Salt tolerance of symbiotic nitrogen ®xation reportedly depends both on the plant and on Rhizobium genotypes (Pessarakli and Zhou 1990;Cordovilla et al 1995). Studies on cultivar±strain interactions indicate that the plant is a determining factor for symbiosis tolerance (Craig et al 1991;Soussi et al 1999), although the salt-tolerant Bradyrhizobium strain ®xes more nitrogen than the sensitive one (Elsheikh and Wood 1990), and there is a marked difference in nodulation and nitrogen ®xation parameters between salt-tolerant and salt-sensitive strains (Kumar et al 1999).…”

Section: Introductionmentioning

confidence: 99%

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Effects of salinity on protein and lipopolysaccharide pattern in a salt-tolerant strain of Mesorhizobium ciceri

et al. 2001

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Aims: To characterize the physiological and metabolic responses of Mesorhizobium ciceri strain ch-191 to salt stress, investigating the changes induced by salinity in protein and lipopolysaccharide pro®les, as well as determining the accumulation of amino acids, glutamate and proline. Methods and Results: Strain ch-191 of M. ciceri was grown with different NaCl concentrations. Protein and lipopolysaccharide patterns were determined by electrophoresis. The strain ch-191 tolerated up to 200 mmol l )1 NaCl, although higher salt dosages limited its growth and induced changes in the protein pro®le. The most noteworthy change in the LPS-I pattern was the decrease in the slowest band and the appearance of an intermediate mobility band. The accumulation of proline in response to salt stress surpassed that of glutamate. Conclusions: The protein pro®le showed major alterations at salinity levels which inhibited growth. However, the alterations in the LPS pro®le and accumulation of compatible solutes were evident from the lowest levels, suggesting that these changes may constitute adaptative responses to salt, allowing normal growth. Signi®cance and Impact of the Study: The selection and characterization of salt-tolerant strains, which also show ef®cient symbiotic performance under salinity, may constitute a strategy for improving Cicer arietinum±Mesorhizobium ciceri symbiosis in adverse environments.

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“…Salt tolerance of symbiotic nitrogen fixation reportedly depends both on the plant and on Rhizobium genotypes (Pessarakli and Zhou 1990; Cordovilla et al . 1995).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of salinity on protein and lipopolysaccharide pattern in a salt-tolerant strain of Mesorhizobium ciceri

et al. 2001

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“…Thus, the infection process seems to be the most sensitive to salt [33,41]. Nevertheless, a large genetic variability in salt tolerance was found among legume species and lines [6,18].…”

Section: Introductionmentioning

confidence: 99%

Genotypic variability for tolerance to salinity of N₂-fixing common bean (Phaseolus vulgaris)

Saadallah¹,

Drevon

Hajji

et al. 2001

Agronomie

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The common bean (Phaseolus vulgaris L.) is often subject to various environmental constraints in the soil. One of these constraints is salinity which is a major limitation for grain legumes yield, especially when the plant growth depends upon N 2 fixation. In order to confirm the variability of the response to moderate salinity, and identify the morphological and physiological criteria associated with tolerance, 9 common bean lines (BAT477, Coco blanc, DOR585, ABA16, NAG310, Flamingo, BRB17, Candide and Striker) were inoculated with Rhizobium tropici CIAT899, and grown in a glasshouse with or without 25 mM NaCl on sterilized sand. A genotypic variation in tolerance to salt was found: ABA16 and BRB17 were the most sensitive lines whereas BAT477 and Flamingo were the most tolerant. The estimated parameters of growth and symbiotic nitrogen fixation showed that (i) some lines which exhibited vigorous growth under the control treatment were greatly affected by salt treatment, (ii) the relative tolerance of some lines to salt seems to depend on the ability to maintain an adequate leaf area and to develop an abundant and efficient nodular system. Phaseolus vulgaris / salinity / N 2 fixation / genotypic variability Résumé-Variabilité génotypique de la tolérance au sel chez le haricot (Phaseolus vulgaris), en condition de fixation symbiotique de N 2. Le haricot (Phaseolus vulgaris L.) est souvent soumis dans le sol à diverses contraintes environnementales. L'une des ces contraintes est la salinité qui représente une limitation majeure du rendement des légumineuses à graines, particulièrement quand la croissance des plantes dépend de la fixation symbiotique de l'azote. Pour confirmer la variabilité de la réponse à une salinité modérée et identifier les critères morphologiques et physiologiques associés à la tolérance, 9 lignées de haricot (BAT477, Coco blanc, DOR585, ABA16, NAG310, Flamingo, BRB17, Candide et Striker) sont inoculées avec une souche efficiente Rhizobium tropici CIAT899, et cultivées sur sable stérile sous serre vitrée en absence ou en présence de 25 mM NaCl. Une variation génotypique dans la tolérance au sel a été observée : ABA16 et BRB17 sont les lignées les plus sensibles alors que BAT477 et Flamingo sont les plus tolérantes. Les paramètres estimés relatifs à la croissance et à la fixation symbiotique de l'azote montrent que (i) certaines lignées exprimant une croissance vigoureuse en milieu témoin sont sévèrement affectées par le traitement salin, (ii) la relative tolérance au sel d'autres lignées repose particulièrement sur leur aptitude à maintenir une importante surface foliaire et à développer un système nodulaire abondant et efficace. Phaseolus vulgaris / salinité / fixation symbiotique de N 2 / variabilité génotypique

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“…The success of such an approach may require the improvement of both partners of the symbiosis, although the microsymbiont is generally more salt-tolerant than the host plant [30]. Considerable variability in salt-tolerance has been reported among and within legume species [5,20,23,27,32]. According to Bliss [3], the limitation of N 2 fixation imposed by environmental factors could be resolved through selection and breeding of improved common bean cultivars.…”

Section: Introductionmentioning

confidence: 99%

Genotypic difference in the short-term response of nitrogenase activity (C₂H₂ reduction) to salinity and oxygen in the common bean

et al. 2001

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difference in the short-term response of nitrogenase activity (C_2H_2 reduction) to salinity and oxygen in the common bean. Agronomie, EDP Sciences, 2001, 21 (6- Abstract -Four genotypes of common bean (Phaseolus vulgaris L.) differing in seed color, were grown hydroponically in a growth chamber to study the short-term effects of 0.1 M NaCl on their symbiotic acetylene reducing activity (ARA). For all genotypes, the ARA was reduced dramatically during the first hour following NaCl application. This was followed by a slower decrease during the next 5 h during which genotypical differences in response to salinity were found. When expressed as percent of the control, the redseed genotype was significantly less inhibited by NaCl than the three others. For all genotypes, the increase in ARA after raising rhizosphere pO 2 was affected by the NaCl treatment. With NaCl the red-seed genotype showed the highest stimulation by pO 2 , which was associated with its higher tolerance to NaCl application. However for all genotypes, raising pO 2 did not allow a complete recovery from the NaCl effect. It is concluded that nitrogenase inhibition by NaCl application in common bean varies among genotypes, and is associated with a short-term nodule limitation by O 2 diffusion, although other mechanisms may also be involved in nodule inhibition by NaCl. N 2 fixation / oxygen diffusion / Phaseolus vulgaris / Rhizobium / salinity Résumé -Effets à court terme de la salinité sur l'activité nitrogénase nodulaire et sa réponse à l'oxygène chez le haricot. Quatre génotypes de haricot (Phaseolus vulgaris L.) différant par la couleur des téguments de leur graine, ont été cultivés hydroponiquement en chambre de culture afin d'étudier les effets de NaCl sur leur fixation symbiotique d'azote et leur croissance. L'activité réductrice de l'acétylène (ARA) était fortement réduite pendant la première heure suivant l'application de NaCl. Cette baisse initiale était suivie d'une baisse plus lente durant laquelle des différences variétales ont été mesurées. Le génotype R était significativement moins inhibé par NaCl que les 3 autres. Pour tous les génotypes, la stimulation d'ARA par augmentation de la pO 2 rhizosphérique était modifiée par le traitement salin. Avec NaCl, le génotype R présenta la plus forte stimulation par la pO 2 , qui était associée à sa plus forte tolérance à NaCl. Toutefois quel que soit le génotype, l'élévation de pO 2 ne compensa pas totalement l'inhibition par NaCl. Il est conclu que l'inhibition de la nitrogénase par la salinité chez le haricot fixateur d'azote varie entre génotypes, et est associée à une limitation du métabolisme nodulaire par un manque d'O 2 , bien que d'autres mécanismes d'inhibition par NaCl puissent être impliqués.

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Effect of salt stress on nitrogen fixation by different cultivars of green beans

Cited by 11 publications

References 19 publications

Effects of salinity on protein and lipopolysaccharide pattern in a salt-tolerant strain of Mesorhizobium ciceri

Effects of salinity on protein and lipopolysaccharide pattern in a salt-tolerant strain of Mesorhizobium ciceri

Genotypic variability for tolerance to salinity of N₂-fixing common bean (Phaseolus vulgaris)

Genotypic difference in the short-term response of nitrogenase activity (C₂H₂ reduction) to salinity and oxygen in the common bean

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Effect of salt stress on nitrogen fixation by different cultivars of green beans

Cited by 11 publications

References 19 publications

Effects of salinity on protein and lipopolysaccharide pattern in a salt-tolerant strain of Mesorhizobium ciceri

Effects of salinity on protein and lipopolysaccharide pattern in a salt-tolerant strain of Mesorhizobium ciceri

Genotypic variability for tolerance to salinity of N2-fixing common bean (Phaseolus vulgaris)

Genotypic difference in the short-term response of nitrogenase activity (C2H2 reduction) to salinity and oxygen in the common bean

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Genotypic variability for tolerance to salinity of N₂-fixing common bean (Phaseolus vulgaris)

Genotypic difference in the short-term response of nitrogenase activity (C₂H₂ reduction) to salinity and oxygen in the common bean