Effect of salt stress on genes encoding translation-associated proteins in<i>Arabidopsis thaliana</i>

Omidbakhshfard, Mohammad Amin; Omranian, Nooshin; Ahmadi, Farajollah Shahriari; Nikoloski, Zoran; Mueller‐Roeber, Bernd

doi:10.4161/psb.21218

Cited by 49 publications

(29 citation statements)

References 46 publications

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“…They are the 40S ribosomal proteins S25-1 (B6SX33), 40S (B6T8K2), S27a (B6TSG5), S8 (C0PCV2), the 60S ribosomal protein L12 (B6TDJ0) and the ribosomal protein L19 (B4FL64). It was reported that the de novo synthesis of ribosomes closely correlates to (i) changing growth rates and (ii) to an increased ability to synthesize proteins (35)(36)(37)(38). Fig.…”

Section: Ribosomal Proteinsmentioning

confidence: 94%

Transient alkalinization of the leaf apoplast stiffens the cell wall during onset of chloride salinity in corn leaves

Geilfus

Tenhaken

Carpentier

2017

Journal of Biological Chemistry

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During chloride salinity, the pH of the leaf apoplast (pHapo) transiently alkalizes. There is an ongoing debate about the physiological relevance of these stress-induced pHapo changes. Using proteomic analyses of expanding leaves of corn (Zea mays L.), we show that this transition in pHapo conveys functionality by (i) adjusting protein abundances and (ii) affecting the rheological properties of the cell wall. pHapo was monitored in planta via microscopy-based ratio imaging, and the leaf-proteomic response to the transient leaf apoplastic alkalinization was analyzed via ultra-high-performanceliquid-chromatography-MS. This analysis identified 1459 proteins, of which 44 exhibited increased abundance specifically through the chloride-induced transient rise in pHapo. These elevated protein abundances did not directly arise from high tissue concentrations of Cl -or Na + but were due to changes in the pHapo. Most of these proteins functioned in growth-relevant processes and in the synthesis of cell wallbuilding components such as arabinose. Measurements with a linear-variable differential transducer revealed that the transient alkalinization rigidified (i.e. stiffened) the cell wall during the onset of chloride salinity. A decrease in t-coumaric-and t-ferulicacid indicates that the wall stiffening arises from cross-linkage to cell wall polymers. We conclude that the pH of the apoplast represents a dynamic factor that is mechanistically coupled to cellular responses to chloride stress. By hardening the wall, the increased pH abrogates wall loosening required for cell expansion and growth. We conclude that the transient alkalinization of the leaf apoplast is related to salinity-induced growth reduction.

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Section: Ribosomal Proteinsmentioning

confidence: 94%

Transient alkalinization of the leaf apoplast stiffens the cell wall during onset of chloride salinity in corn leaves

Geilfus

Tenhaken

Carpentier

2017

Journal of Biological Chemistry

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“…The salt treatment experiments were performed as reported (Balazadeh et al, 2010a;Omidbakhshfard et al, 2012;Allu et al, 2014). For nitrogen depletion experiments, Arabidopsis (Col-0) seeds were surface sterilized.…”

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confidence: 99%

The Arabidopsis transcription factor MYB112 promotes anthocyanin formation during salinity and under high light stress

Lotkowska

Tohge²,

Fernie³

et al. 2015

Plant Physiol.

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136

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“…Arabidopsis thaliana (L.) Heynh. (Col‐0) seeds were obtained from the A. thaliana Resource Centre for Genomics (INRA, France; http://dbsgap.versailles.inra.fr/publiclines/) and plants were cultured as reported (Omidbakhshfard et al ). In brief, seeds were surface‐sterilized using sodium hypochlorite solution (50%), sown on agar medium plates (1× Murashige‐Skoog (MS), 1% sucrose, pH 5.8), and plates were stored for 2 d under vernalization condition and then transferred to long‐day growth condition (16 h light, 22 °C and 8 h dark, 18 °C).…”

Section: Methodsmentioning

confidence: 99%

A step‐by‐step protocol for formaldehyde‐assisted isolation of regulatory elements from Arabidopsis thaliana

Omidbakhshfard

Winck

Arvidsson

et al. 2014

JIPB

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The control of gene expression by transcriptional regulators and other types of functionally relevant DNA transactions such as chromatin remodeling and replication underlie a vast spectrum of biological processes in all organisms. DNA transactions require the controlled interaction of proteins with DNA sequence motifs which are often located in nucleosome-depleted regions (NDRs) of the chromatin. Formaldehyde-assisted isolation of regulatory elements (FAIRE) has been established as an easy-to-implement method for the isolation of NDRs from a number of eukaryotic organisms, and it has been successfully employed for the discovery of new regulatory segments in genomic DNA from, for example, yeast, Drosophila, and humans. Until today, however, FAIRE has only rarely been employed in plant research and currently no detailed FAIRE protocol for plants has been published. Here, we provide a step-by-step FAIRE protocol for NDR discovery in Arabidopsis thaliana. We demonstrate that NDRs isolated from plant chromatin are readily amenable to quantitative polymerase chain reaction and next-generation sequencing. Only minor modification of the FAIRE protocol will be needed to adapt it to other plants, thus facilitating the global inventory of regulatory regions across species.

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Effect of salt stress on genes encoding translation-associated proteins inArabidopsis thaliana

Cited by 49 publications

References 46 publications

Transient alkalinization of the leaf apoplast stiffens the cell wall during onset of chloride salinity in corn leaves

Transient alkalinization of the leaf apoplast stiffens the cell wall during onset of chloride salinity in corn leaves

The Arabidopsis transcription factor MYB112 promotes anthocyanin formation during salinity and under high light stress

A step‐by‐step protocol for formaldehyde‐assisted isolation of regulatory elements from Arabidopsis thaliana

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