Effect of Salvia officinalis and S. sclarea on rats with a high-fat hypercaloric diet

Abstract

Phytotherapy for the correction of excess body weight is widely used. However, a comprehensive study of herbal preparations on the organism of model animals has been carried out only for a few plant species. Supplementing the diet of rats with closely related sage species (Salvia officinalis L. and S. sclarea L.) against the background of high-fat hypercaloric diet triggered multidirectional changes in their metabolism. The addition of crushed dry shoots of S. officinalis to the diet of animals led to a sharp increase in their body weight (up to 130.8% of the initial one in 30 days of the experiment). The body weight of the rats treated with S. sclarea for 30 days increased only up to 103.8% of their initial weight and was lower than in the control group. Addition of S. officinalis caused an increase in daily weight gain up to 253.1% of the control group, and S. sclarea – its decrease to 27.8% of the daily weight gain in the control group. In the S. officinalis group, the relative weight of the brain, spleen, and thymus decreased, while in the S. sclarea group, the relative weight of the thymus decreased and that of the colon increased. Under the influence of S. officinalis, the concentration of urea, total bilirubin, and triglycerides in the blood plasma of male rats decreased and the concentration of total protein and the activity of alkaline phosphatase increased. While consuming S. sclarea shoots, there was an increase of alkaline phosphatase activity in the rats’ blood, but atherogenic index (23.1% of the level of the control group) sharply dropped due to an increase in the concentration of high-density lipoprotein cholesterol (286.9% of the control) and a decrease in the concentration of low-density lipoprotein cholesterol (67.7% of control). In rats feeding on S. sclarea shoots, we observed a decrease in the concentration of triglycerides in the blood (39.9% of the control), a decrease in the activity of gamma-glutamyl transferase (62.8%), and an increase in the Ca/P ratio (132.5% of the control group). No significant changes were observed in CBC and WBC differential of male rats when eating S. officinalis and S. sclarea shoots. According to the results of the open field test, the physical and orientational activity of male rats under the influence of S. officinalis significantly decreased by the end of the experiment. Emotional status of rats, on the contrary, decreased when they ate dry crushed shoots of S. sclarea in the composition of the food. Thus, excess body weight of rats in the conditions of hypercaloric diet led to more pronounced deviations from the norm while consuming dry crushed shoots of S. officinalis. The addition of S. sclarea dry crushed shoots to the animals’ diet normalized the body weight in comparison with the control group, reduced the negative manifestations of obesity at the biochemical and organismal levels. In this regard, the substances that contains S. sclarea should be carefully studied for anti-atherosclerotic activity, and tea supplemented with S. sclarea shoots can be recommended as a corrective supplement in the diet of overweight people.

The effect of a new feed additive of humic nature on morphometric indicators of laboratory rats depending on the dose

“…In the S. sclarea group, the relative mass of the thymus decreased (43.4 %), and the relative mass of the colon increased (159.7 % compared to the control group). The consumption of sage led to a significant decrease in the relative weight of the organs of the immune system (thymus gland and spleen), but no changes in blood composition (total number of leukocytes and leukoformula) were found (Lieshchova et al, 2021).…”

Effect of Rhodiola rosea Rhizomes and Punica granatum Fruit Peel on the Metabolic Processes and Physiological Activity of Rats Fed with Excessive Fat Diet

“…The main components of this diet were crushed in a mill (such as grain, meat, bone meal and mineral-vitamin complex), mixed, then sunflower oil was added and granules were made from this mixture (feed granulator GKM-100; Tekhnomashstroy, Cherkasy, Ukraine) at the mass of 4200 g for each group during the whole duration of the experiment. Since in our previous studies of other plant species ( 23 ) the duration of the experiment was 30 days, to ensure the possibility of comparison, the duration of this experiment was the same. Crops like fresh roots, in the appropriate amount, were additionally given every day.…”

Вплив Лікарських Рослин Роду Salvia На Морфофункціональний Стан Печінки Щурів На Тлі Високожирового Раціону

“…Відновлюючий вплив ефірної олії шавлії на тканину печінки при її ушкоджені встановили і Durgha et al ( 2016) [3]. В науковій літературі є повідомлення про застосування препаратів шавлії для стимуляції імунної системи, її вплив на обмін речовин, а екстракт із листя, при застосуванні в експерименті, показав гіпоглікемічну і гіполіпідемічну активність [10]. Стаття написана на основі тез доповіді конференції [12].…”

“…В науковій літературі є повідомлення про застосування препаратів шавлії для стимуляції імунної системи, її вплив на обмін речовин, а екстракт із листя, при застосуванні в експерименті, показав гіпоглікемічну і гіполіпідемічну активність [10]. Стаття написана на основі тез доповіді конференції [12]. Мета дослідження -виявити і порівняти вплив двох видів шавлії лікарської (S. officinalis) і мускатної (S. sclarea) на морфофункціональний стан печінки лабораторних щурів на тлі споживання ними високожирового раціону.…”