Effect of Sampling and Short Isolation Methodologies on the Recovery of Human PathogenicYersinia enterocoliticafrom Pig Tonsils

Evaluation of the ISO 10273:2003 method for the isolation of human pathogenic Yersinia enterocolitica from pig carcasses and minced meat

“…Similar to the present study, PSB enrichment was more efficient than ITC enrichment for isolation of Y. enterocolitica from porcine tonsil swabs, albeit different sample sizes and dilutions were used in both media (Van Damme et al, 2012). In contrast, for tonsillar tissue samples, ITC was slightly, yet not significantly better than PSB enrichment.…”

Controlled Experimental Infection in Pigs with a Strain of Yersinia enterocolitica Harboring Genetic Markers for Human Pathogenicity: Colonization and Stability

“…However, the transient detection in digestive tract and internal organs could correspond to a presence/absence of Ye in organs or a variation of the number of Ye organisms (low versus high number). It is indeed possible to quantify Ye in highly contaminated samples using direct plating ( 28 , 33 ). However, in samples with low levels of contamination, an enrichment step is needed, especially in the presence of significant background flora, such as in feces or intestinal contents.…”

Diagnosis of Yersinia enterocolitica serovar O:9 in a commercial 2400-sow farm with false-positive Brucella suis serology using western blot, competitive ELISA, bacterial isolation, and whole genome sequencing

“…Swabbing the tonsils of swine has been shown to be a pos sible method of isolating Y enterocolitica from carrier swine. 18,19 Reports of similar cases in species other than swine have cultured Y enterocolitica from the feces of infected ani mals. 20,21 However, these methods would not rule out the potential for dual infection with Yersinia and Brucella, and therefore would not be a suitable test for ruling out FPSRs.…”