Effect of Saponins of Albizia lebbeck (L.) Benth bark on the reproductive system of male albino rats

Gupta, R.; Chaudhary, Rubina; Yadav, Rajesh Kumar; Verma, Suresh K.; Dobhal, M. P.

doi:10.1016/j.jep.2004.07.025

Cited by 83 publications

(46 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This result is supported by other findings in that drug-induced nephrotoxicity may be gender related [28]. The serum levels of creatinine and urea in different druginduced nephrotoxicity were higher in males than females [28][29][30][31]. Usually urea is increased in acute and chronic intrinsic renal diseases, which is characterized by decrement in effective circulating blood volume within the kidney [32].…”

Section: Discussionsupporting

confidence: 80%

Evaluations of Biochemical, Hematological and Histopathological Parameters of Subchronic Administration of Ethanol Extract of Albizia Gummifera Seed in Albino Wistar Rat

Debebe¹,

Afework²,

Makonnen³

et al. 2017

J Clinic Toxicol

View full text Add to dashboard Cite

Albizia gummifera are plants found in Ethiopia that have different medicinal values.The objective of the present study was to evaluate the toxicological effects of sub-chronic administered hydro-ethanolic (70%) seeds extract of Albizia gummiferain albino Wistar rats. The seeds of these plants were collected from different areas of Ethiopia. They were dried and crushed to powder and macerated with hydro-alcohol and placed in orbital shaker. The extract was then filtered through Whatman filter paper No.1 and the filtrate was evaporated to dryness by Rota vapor and further concentrated by water bath at 40ºC. The extract was packed in air tight brown glass bottles and kept in a refrigerator at 4ºC. The extract was then administered to rats at different doses to determine the LD 50 of the extract and at doses of 125 mg/kg/day and 250 mg/kg/day for the sub-chronic toxicity study. The LD 50 of Albizzia gummifera were found as 4000 mg/kg and 3500 mg/kg, respectively. Statistically significant difference in body weight was observed in female rats in the 10 th week at 250 mg/kg body weight of seeds extract of Albizia gummifera administered group and in the male rats at lowest dose during the 9 th and 10 th weeks of administration period for seeds extract of Millettia ferruginea.The seeds extract of Albizia gummifera statistically decreased (p ≤ 0.05) MCHin the male rats at both doses; MCHC at both 125 mg/kg and 250 mg/kg in the female rats; and MCH in the female rats at higher dose and increased RDW-CV in the male rats at both doses. It increased NEUT at the highest dose in both females and males. The seeds extract of Millettia ferruginea decreased (p ≤ 0.05) in the MCHC and MONO of female rats at the highest doses. ALP, ALT and urea were found significant in the female rats administered with 250 mg/kg of seeds extract. While, seeds extract of Albizia gummifera increased only urea in male rats at 250 mg/kg. Some histopathological changes in liver and kidney were also observed for both plants extracts. There were inflammations, congestions and focal hepatocellular necrosis of the liver tissue. The extracts also produced atrophyof the glomeruli of the kidney. The observed changes in both of the plant seeds extract might have resulted because of the presence of some bioactive ingredients in the extract. Therefore, the active ingredients which might be responsible for toxic insult should be researched with their mechanisms of actions.

show abstract

Section: Discussionsupporting

confidence: 80%

Evaluations of Biochemical, Hematological and Histopathological Parameters of Subchronic Administration of Ethanol Extract of Albizia Gummifera Seed in Albino Wistar Rat

Debebe¹,

Afework²,

Makonnen³

et al. 2017

J Clinic Toxicol

View full text Add to dashboard Cite

show abstract

“…Leydig cell aromatase activity is under luteinizing hormone (LH) control and that paracrine factors of Sertoli cell origin are also involved (Carreau & Levallet 2000;Genissel et al 2001). In addition, Gupta et al (2005) reported that A. lebbeck inhibited the fertility of male rats which suppressed the function of Leydig cells and Sertoli cells and inhibited sperm production. Whereas Paoletta et al (2008) noted that the aglycone myricetin inhibits the aromatase enzyme.…”

Section: Discussionmentioning

confidence: 99%

“…Albizia lebbeck (L.) Benth. seeds, pods and bark methanol extracts were reported to possess antispermatogenic and antiandrogenic activities in male rats (Gupta et al 2005). Recently, Farag et al (2013) reported that saponins of A. lebbeck seeds exhibited antiovulatory properties in female rats: the seed extract had an antifertility effect on male rats (Singh et al 1991).…”

Section: Introductionmentioning

confidence: 99%

Myricitrin and bioactive extract ofAlbizia amaraleaves: DNA protection and modulation of fertility and antioxidant-related genes expression

Kassem

Ibrahim

Hussein

et al. 2016

Pharmaceutical Biology

View full text Add to dashboard Cite

Context: Albizia species are reported to exhibit many biological activities including antiovulatory properties in female rats and antispermatogenic and antiandrogenic activities in male rats. Objective: The present study investigates the flavonoids of Albizia amara (Roxb.) B. Boivin (Fabaceae) leaves and evaluates their activity on gene expression of fertility and antioxidant glutathione-S-transferaserelated genes of treated female mice in addition to their effect on DNA damage. Materials and methods: Plant materials were extracted by using 70% methanol for 48 h, the extract was chromatographed on a polyamide 6S column, each isolated compound was purified by using Sephadex LH-20 column; its structure was elucidated by chemical and spectral methods. Both the leaves extract and myricitrin (200, 30 mg/kg bw/d, respectively) were assayed for their effect on DNA damage in female mice after four weeks treatment using Comet assay. Their modulatory activity on gene expression of fertility (aromatase CYP19 and luteinizing hormone LH) and antioxidant glutathione-S-transferase (GST)-related genes of treated female mice were investigated by real-time PCR (qPCR). Results: Quercetin-3-O-gentiobioside, myricitrin, quercetin-3-O-a-rhamnopyranoside, myricetin, quercetin and kaempferol were isolated and identified from the studied taxa. Myricitrin and the extract induced low rate of DNA damage (4.8% and 5%, respectively), compared with the untreated control (4.2%) and significantly down-regulated the expression of CYP19 and LH genes and up-regulated GST gene. Discussion and conclusion: Our results highlight the potential effect of the leaves extract of Albizia amara and myricitrin as fertility-regulating phytoconstituents with ability to protect DNA from damage and cells from oxidative stress. ARTICLE HISTORY

show abstract

“…The observations related to the unique nature of the change in the seminiferous tubules are similar as in every plant assayed so far in male anti-fertility perspective. Viz., Carica papaya (Lohiya and Goyal, 1992); Euphorbia nerrifolia (Mali and Chaturvedi, 1994); Azadirachta indica (Kasturi et al, 1997); Momordica charantia (Naseem et al, 1998); Coculus pendulus (Verma and Lall, 1999); methanol stem extract of Sarcostemma acidum (Roxb) Voigt (Verma et al, 2002); stem extract of Tinospora cordifolia (Willd) (Gupta and Sharma, 2003); ethanol extract of Semecarpus anacardium ; and saponins isolated from Albizia lebbeck bark (Gupta et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Reversible histoarchitecture study of testis and cauda epididymis and changes in cauda epididymal epithelial cell types on treatment with benzene extract of Ocimum sanctum leaves in albino rats

Ahmed¹,

Ahamed²,

Aladakatti³

et al. 2008

Oriental Pharmacy and Experimental Medicine

View full text Add to dashboard Cite

SUMMARYIn the present study, an attempt has been made to assess whether the effect of benzene extract of Ocimum sanctum leaves on the ultrastructural changes in the epithelial cells of the cauda epididymis, its subsequent recovery in the seminiferous epithelium and fertility of male albino rats. Wistar strain male albino rats were orally administered benzene extract of 250 mg/kg body weight of O. sanctum leaves followed by subsequent recovery maintaining suitable controls for 48 days. Results indicate decrease in the weights of testis, epididymis and seminal vesicles. Other accessory organs were not affected. Total count, cell and nuclei diameters of germ cells and Leydig cells were reduced. Cauda epididymis exhibited significant reduction in epithelial height and nuclei diameter of epithelial cells. Cells showed vacuolization with exhibit of signs of degeneration. Ultra study revealed that, in general, the cauda epididymis was affected and in particular, the principal, clear and basal cells were highly disturbed. Further, there was decrease in the size of lipid droplets, mitochondria, Golgi complex, endoplasmic reticulum and accumulation of lysosomal bodies. Fertility performance test showed no implantation in female rats mated with O. sanctum treated rats. Moreover, their recovery after withdrawal of treatment was observed suggesting that the effect of the treatment is transient and reversible. A recovery period resulted in normal spermatogenesis and fertility, suggesting reversible antispermatogenic and antifertility effects of the plant.

show abstract

Effect of Saponins of Albizia lebbeck (L.) Benth bark on the reproductive system of male albino rats

Cited by 83 publications

References 27 publications

Evaluations of Biochemical, Hematological and Histopathological Parameters of Subchronic Administration of Ethanol Extract of Albizia Gummifera Seed in Albino Wistar Rat

Evaluations of Biochemical, Hematological and Histopathological Parameters of Subchronic Administration of Ethanol Extract of Albizia Gummifera Seed in Albino Wistar Rat

Myricitrin and bioactive extract ofAlbizia amaraleaves: DNA protection and modulation of fertility and antioxidant-related genes expression

Reversible histoarchitecture study of testis and cauda epididymis and changes in cauda epididymal epithelial cell types on treatment with benzene extract of Ocimum sanctum leaves in albino rats

Contact Info

Product

Resources

About