Effect of Sarcocystosis Infection on Cytogenetic Peripheral Blood Lymphocytes in Ewes

Abstract

Aim of this study was to estimate the potential genotoxoicity of sarcocystiosis on cytogenetic of infected ewes lymphocystes by using different cytogenetic test. Forty ewes infected with sarcocystiosis and 20 healthy were collected as an uninfected group. Lymphocytes from peripheral blood of infected and uninfected ewes were used for culture and subsequent cytogenetic studies. Cytogenetic analysis was used as a marker to reflect the genotoxic effect of sarcocystosis parasite on the lymphocytes. The mean Mitotic Index (MI) of infected ewes was significantly lower (P≤ 0.05) than of control group, while mean replicative index (RI) of infected ewes was non-significant as compared with uninfected group. Sister Chromatid Exchange (SCEs) and Chromosomal Aberrations (CAs) showed significant (P≤ 0.05) increase in infected ewes as compared with control group.

In Vitro Genotoxic Effects of Sarcocystis gigantea Cystizoites Acetone Powder Extract on Sheep Lymphocytes

“…Previous studies highlighted the toxicity of this parasite on the host (4,5) and eventually, its pathological and clinical effects (2,6). Other researcher pointed out that Sarcocystis parasite had potential genotoxic effects on sheep lymphocyte, In Vivo, as an intermediate host through the infection course directly (7,8).…”