Effect of scaffold properties on adhesion and maintenance of boundary cap neural crest stem cells in vitro

Han, Yilin; Baltriukienė, Daiva; Kozlova, Elena N.

doi:10.1002/jbm.a.36900

Cited by 10 publications

(9 citation statements)

References 27 publications

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“…Moreover, Nestin positive cells decreased over time for all cell lines except for hNSC#1: this is likely ascribable to an initially higher proliferation rate (until 1WIV) caused by an enriched percentage of stem/progenitor cells population that progressively faded into differentiating (or fate-committed) cells ( Figure 4G). To note, the obtained values can be easily modified with further customizations of HYDROSAP depending on the regenerative application: indeed we already demonstrated that by tuning different key experimental parameters (e.g., matrix stiffness, presence of functional motifs, and/or addition of neurotrophic factors) it is possible to modulate NSCs differentiation and improve differentiation toward neuronal fate (Jain et al, 2020): for example, we showed that increments of cell proliferation rate and neuronal differentiation is inversely proportional to the matrix stiffness, while stiffness and astrocytes number directly correlated (Cunha et al, 2011;Caprini et al, 2013;Han et al, 2020;Pandamooz et al, 2020). In future experiments, it will also be interesting to assess the influence of time-evolving viscoelastic properties of hydrogels over cellular behavior (Mattei et al, 2020).…”

Section: Discussionmentioning

confidence: 90%

Multi-Functionalized Self-Assembling Peptides as Reproducible 3D Cell Culture Systems Enabling Differentiation and Survival of Various Human Neural Stem Cell Lines

2020

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Neural stem cells-based therapies have shown great potential for central nervous system regeneration, with three-dimensional (3D) culture systems representing a key technique for tissue engineering applications, as well as disease modeling and drug screenings. Self-assembling peptides (SAPs), providing biomimetic synthetic microenvironments regulating cellular functionality and tissue repair, constitute a suitable tool for the production of complex tissue-like structures in vitro. However, one of the most important drawbacks in 3D cultures, obtained via animal-derived substrates and serumrich media, is the reproducibility and tunability of a standardized methodology capable to coax neural differentiation of different human cell lines. In this work we cultured four distinct human neural stem cell (hNSC) lines in 3D synthetic multifunctionalized hydrogel (named HYDROSAP) for up to 6 weeks. Three-dimensional cultures of differentiating hNSCs exhibited a progressive differentiation and maturation over time. All hNSCs-derived neurons in 3D culture system exhibited randomly organized entangled networks with increasing expression of GABAergic and glutamatergic phenotypes and presence of cholinergic ones. Oligodendrocytes formed insulating myelin sheaths positive for myelin basic protein (MBP). In summary, results demonstrated a successfully standardized and reproducible 3D cell culture system for hNSC differentiation and maturation in serum-free conditions useful for future therapies.

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Section: Discussionmentioning

confidence: 90%

Multi-Functionalized Self-Assembling Peptides as Reproducible 3D Cell Culture Systems Enabling Differentiation and Survival of Various Human Neural Stem Cell Lines

2020

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“…[ 350 , 351 , 352 ] Because the metal oxides are nonedible, scaffolds containing these coatings can be used only as temporary supports during the proliferation phase and will not be present in the final CM product. The stiffness, roughness, and wettability of the culture surface can also influence cell adhesion, [ 353 , 354 ] providing additional opportunities for CM producers to tune the adhesive properties of their scaffolding materials.…”

Section: Engineering Biological and Structural Complexitymentioning

confidence: 99%

Scaffolding Biomaterials for 3D Cultivated Meat: Prospects and Challenges

Bomkamp¹,

Skaalure²,

Fernando³

et al. 2021

Advanced Science

156

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Cultivating meat from stem cells rather than by raising animals is a promising solution to concerns about the negative externalities of meat production. For cultivated meat to fully mimic conventional meat's organoleptic and nutritional properties, innovations in scaffolding technology are required. Many scaffolding technologies are already developed for use in biomedical tissue engineering. However, cultivated meat production comes with a unique set of constraints related to the scale and cost of production as well as the necessary attributes of the final product, such as texture and food safety. This review discusses the properties of vertebrate skeletal muscle that will need to be replicated in a successful product and the current state of scaffolding innovation within the cultivated meat industry, highlighting promising scaffold materials and techniques that can be applied to cultivated meat development. Recommendations are provided for future research into scaffolds capable of supporting the growth of high-quality meat while minimizing production costs. Although the development of appropriate scaffolds for cultivated meat is challenging, it is also tractable and provides novel opportunities to customize meat properties.

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“…Previous studies have underlined the effect of a scaffold's mechanical properties on the seeded cells. [ 15,16 ] Many cells are mechanosensitive, making them responsive to the mechanical cues from the environment. Surface morphology and stiffness are parameters that could modulate cell behavior.…”

Section: Requirements and Challengesmentioning

confidence: 99%

Biomaterial Properties Modulating Bone Regeneration

Zhu

Goh

Shrestha

2021

Macromolecular Bioscience

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Biomaterial scaffolds have been gaining momentum in the past several decades for their potential applications in the area of tissue engineering. They function as three‐dimensional porous constructs to temporarily support the attachment of cells, subsequently influencing cell behaviors such as proliferation and differentiation to repair or regenerate defective tissues. In addition, scaffolds can also serve as delivery vehicles to achieve sustained release of encapsulated growth factors or therapeutic agents to further modulate the regeneration process. Given the limitations of current bone grafts used clinically in bone repair, alternatives such as biomaterial scaffolds have emerged as potential bone graft substitutes. This review summarizes how physicochemical properties of biomaterial scaffolds can influence cell behavior and its downstream effect, particularly in its application to bone regeneration.

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Effect of scaffold properties on adhesion and maintenance of boundary cap neural crest stem cells in vitro

Cited by 10 publications

References 27 publications

Multi-Functionalized Self-Assembling Peptides as Reproducible 3D Cell Culture Systems Enabling Differentiation and Survival of Various Human Neural Stem Cell Lines

Multi-Functionalized Self-Assembling Peptides as Reproducible 3D Cell Culture Systems Enabling Differentiation and Survival of Various Human Neural Stem Cell Lines

Scaffolding Biomaterials for 3D Cultivated Meat: Prospects and Challenges

Biomaterial Properties Modulating Bone Regeneration

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