Effect of Calendula Officinalis Cream on Achilles Tendon Healing

Abstract

In recent years, the scientific community has undertaken research on plant extracts, searching for compounds with pharmacological activities that can be used in diverse fields of medicine. Calendula officinalis L. is known to have antioxidant, anti-inflammatory, antibacterial, and wound healing properties when used to treat skin burns. Therefore, the purpose of this study was to analyze the effects of C. officinalis on the initial phase of Achilles tendon healing. Wistar rats were separated in three groups: Calendula (Cal)-rats with a transected tendon were treated with topical applications of C. officinalis cream and then euthanized 7 days after injury; Control (C)-rats were treated with only vehicle after transection; and Normal (N)-rats without tenotomy. Higher concentrations of hydroxyproline (an indicator of total collagen) and non-collagenous proteins were observed in the Cal group in relation to the C group. Zymography showed no difference in the amount of the isoforms of metalloproteinase-2 and of metalloproteinase-9, between C and Cal groups. Polarization microscopy images analysis showed that the Cal group presented a slightly higher birefringence compared with the C group. In sections of tendons stained with toluidine blue, the transected groups presented higher metachromasy as compared with the N group. Immunocytochemistry analysis for chondroitin-6-sulfate showed no difference between the C and Cal groups. In conclusion, the topical application of C. officinalis after tendon transection increases the concentrations of collagen and non-collagenous proteins, as well as the collagen organization in the initial phase of healing. Anat Rec, 298:428-435, 2015. V C 2014 Wiley Periodicals, Inc.

Evolution of a diabetic foot wound with the use of calendula: A single‐case experimental design

“…In particular, the commercially available product containing the C. officinalis (Plenusdermax) hydrolycollic extract, as it promoted the epithelization of the wound, reducing healing time in patients with venous leg ulcers, since it was this research that stimulated the use of calendula in the treatment of the patient . The results of the present study replicate the findings regarding the healing effect of calendula reported in other studies (Aro et al, 2015;Hartmann et al, 2017). Evidencing, in this case, the potential use in completely necrotic tissues in diabetic foot situation.…”

“…As an example, the description of the ability to potentiate the organization of collagen in the early stage of the healing process associated with an increase in hydroxyproline concentrations, an indicator of collagen content in tissue (Aro et al, 2015;Hartmann et al, 2017).…”

The Effect of Calendula officinalis on Oxidative Stress and Bone Loss in Experimental Periodontitis

“…CLO preserved collagen confirming others studies which already showed that CLO extract reduced collagen breakdown (Millán et al, 2016), and increased collagen concentration (Aro et al, 2015). In gingival fibroblasts, CLO inhibited matrix metalloproteinases (MMP)-2 (Saini et al, 2012).…”

MicroRNAs Associated with Shoulder Tendon Matrisome Disorganization in Glenohumeral Arthritis

“…The collagen fibers of smaller diameter were found increased at the site of the injury which results in the failure of load bearing functions [16]. Such small diameter fibers were reported to be of collagen type 3 and are secreted by fibroblasts/tenoblasts after about 7 days of injury.…”