Effect of SDS Micelles on Rhodamine-B Diffusion in Hydrogels

Haglund, Bert O.; Wurster, Dale Eric; Sundelöf, Lars‐Olof; Upadrashta, Sathyanarayana M.

doi:10.1021/ed073p889

Cited by 20 publications

(13 citation statements)

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“…In the absence of mitigating circumstances, the diffusion coefficient of molecules in a bulk solution should decrease with the cube root of the molecular weight (19). Except for 3K-Dex, this relationship was observed for our biofilms, although the D bf values indicated that diffusion rates in biofilms were much lower than those in bulk water; this attenuation effect was more pronounced at higher probe molecular weights (Table 2).…”

Section: Discussionmentioning

confidence: 66%

“…The data in Table 1 were used to calculate D bf values (Table 2). On theoretical grounds (19), it may be shown that diffusivity is linearly proportional to the cube root of the molecular weight. Therefore, when D bf was plotted against the cube root of the molecular weight, a linear relationship (r 2 ϭ 0.94) was obtained over the molecular weight range of 10,000 through 240,000, but a dramatic increase in diffusivity occurred as the probe molecular weight decreased from 10,000 to 3,000 (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Mass Transport of Macromolecules within an In Vitro Model of Supragingival Plaque

Thurnheer

Gmür

Shapiro

et al. 2003

Appl Environ Microbiol

138

129

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The aim of this study was to examine the diffusion of macromolecules through an in vitro biofilm model of supragingival plaque. Polyspecies biofilms containing Actinomyces naeslundii, Fusobacterium nucleatum, Streptococcus oralis, Streptococcus sobrinus, Veillonella dispar, and Candida albicans were formed on sintered hydroxyapatite disks and then incubated at room temperature for defined periods with fluorescent markers with molecular weights ranging from 3,000 to 900,000. Subsequent examination by confocal laser scanning microscopy revealed that the mean square penetration depths for all tested macromolecules except immunoglobulin M increased linearly with time, diffusion coefficients being linearly proportional to the cube roots of the molecular weights of the probes (range, 10,000 to 240,000). Compared to diffusion in bulk water, diffusion in the biofilms was markedly slower. The rate of diffusion for each probe appeared to be constant and not a function of biofilm depth. Analysis of diffusion phenomena through the biofilms suggested tortuosity as the most probable explanation for retarded diffusion. Selective binding of probes to receptors present in the biofilms could not explain the observed extent of retardation of diffusion. These results are relevant to oral health, as selective attenuated diffusion of fermentable carbohydrates and acids produced within dental plaque is thought to be essential for the development of carious lesions.The structure of microbial biofilms, consisting of single cells, cell aggregates, and microcolonies embedded in an exocellular polymeric hydrogel, has been the subject of intense experimental and theoretical scrutiny over the past decade (10,20,38,46). Biofilm anatomy and the physiological status of the cells contained within biofilms have profound consequences for clinical, industrial, and environmental microbiology. The exocellular space constitutes a primitive microcirculatory system that enables two-way transport between biofilm constituents and their surroundings and that facilitates intrabiofilm communication.Knowledge of the kinetics of mass transport within biofilms is essential for understanding how they achieve their characteristic architectures and for optimizing strategies to control or eradicate biofilms. Transport phenomena in biofilms have been studied by using microelectrodes (3, 41, 42) fiberoptic microsensors (2, 43), nuclear magnetic resonance spectroscopy (1, 52), infrared spectroscopy combined with Raman microscopy (47), fluorescence recovery after photobleaching (4, 5), fluorescence correlation spectroscopy (FCS) (18), and confocal laser scanning microscopy (CLSM) (11,25,26). Indirect methods predicated upon nuclear magnetic resonance spectroscopy, infrared spectroscopy-Raman microscopy, fluorescence recovery after photobleaching, FCS, or CLSM are preferred for measuring mass transport in biofilms, since invasive procedures (e.g., those involving microelectrodes or microsensors) are likely to compromise structural integrity and therefore perturb the del...

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Section: Discussionmentioning

confidence: 66%

Section: Resultsmentioning

confidence: 99%

Mass Transport of Macromolecules within an In Vitro Model of Supragingival Plaque

Thurnheer

Gmür

Shapiro

et al. 2003

Appl Environ Microbiol

138

129

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“…At pH below 4.0, electrostatic interactions between the negatively modi ed surface of Fe 2 O 3 -13X and RBH + molecules will occur, contributing to the high dye removal percentage [31]. On the contrary, at pH above 4.0, the removal mechanism changes because of the formation of the zwitterion, and the combination e ects of positive charge and negative charge of the RB molecules will a ect the removal process [32].…”

Section: E Ect Of the Solution Phmentioning

confidence: 99%

Sodium Dodecyl Sulfate‐Modified Fe₂O₃/Molecular Sieves for Removal of Rhodamine B Dyes

AbdulRazak

Rohani

2018

Advances in Materials Science and Engineering

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Studying the removal of rhodamine B (RB) dye by using zeolite 13X molecular sieves supported by Fe 2 O 3 nanoparticles (denoted as Fe 2 O 3 -13X) is the main objective of this study. Fe 2 O 3 -13X was synthesized and modified by the addition of sodium dodecyl sulfate (SDS). e prepared Fe 2 O 3 -13X was characterized by XRD, TEM, SEM, and zeta potential. e effects of the solution pH, SDS amount, contact time, initial dye concentration, and adsorbent dosage on the removal efficiency of RB were studied. A maximum removal efficiency of 99.3% was achieved. e adsorption equilibrium data of RB were fitted using the Freundlich model, yielding the maximum adsorption capacity of 89.3 mg/g. e findings revealed that the RB adsorption onto Fe 2 O 3 -13X modified with SDS (Fe2O3-13X-Ms) was described by a pseudo-second-order kinetic equation. e results reported in this paper indicate that a high RB removal percentage was attained by adding SDS to Fe 2 O 3 -13X.

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“…LPS). We assume that the antigen and PAMP molecules share similar dynamics, produced at the same rate g(x, t), degrading at the same constant rate m A , diffusing at roughly the same rate D A , as justified by the Stokes-Einstein equation [25], and consumed largely through the indiscriminate process of pinocytosis by macrophages, neutrophils and immature dendritic cells. Henceforth, we treat both molecules as one substance, denoted by A, that satisfies the following partial differential equation, along with initial and boundary conditions.…”

Section: Antigen and Pampmentioning

confidence: 99%

Mathematical Modelling of Immune Response in Tissues

Zhou

Dorman

et al. 2008

Computational and Mathematical Methods in Medicine

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We have developed a spatial -temporal mathematical model (PDE) to capture fundamental aspects of the immune response to antigen. We have considered terms that broadly describe intercellular communication, cell movement, and effector function (activation or inhibition). The PDE model is robust to variation in antigen load and it can account for (1) antigen recognition, (2) an innate immune response, (3) an adaptive immune response, (4) the elimination of antigen and subsequent resolution of the immune response or (5) equilibrium of the immune response to the presence of persistent antigen (chronic infection) and the formation of a granuloma.

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Effect of SDS Micelles on Rhodamine-B Diffusion in Hydrogels

Cited by 20 publications

References 9 publications

Mass Transport of Macromolecules within an In Vitro Model of Supragingival Plaque

Mass Transport of Macromolecules within an In Vitro Model of Supragingival Plaque

Sodium Dodecyl Sulfate‐Modified Fe₂O₃/Molecular Sieves for Removal of Rhodamine B Dyes

Mathematical Modelling of Immune Response in Tissues

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Effect of SDS Micelles on Rhodamine-B Diffusion in Hydrogels

Cited by 20 publications

References 9 publications

Mass Transport of Macromolecules within an In Vitro Model of Supragingival Plaque

Mass Transport of Macromolecules within an In Vitro Model of Supragingival Plaque

Sodium Dodecyl Sulfate‐Modified Fe2O3/Molecular Sieves for Removal of Rhodamine B Dyes

Mathematical Modelling of Immune Response in Tissues

Contact Info

Product

Resources

About

Sodium Dodecyl Sulfate‐Modified Fe₂O₃/Molecular Sieves for Removal of Rhodamine B Dyes