Effect of sealing infected dentin with glass ionomer cement on the abundance and localization of MMP‐2, MMP‐8, and MMP‐9 in young permanent molars <i>in vivo</i>

Kuhn, Eunice; Reis, Alessandra; Campagnoli, Eduardo Bauml; Chibinski, Ana Cláudia Rodrigues; Carrilho, Marcela Rocha de Oliveira; Wambier, Denise Stadler

doi:10.1111/ipd.12167

Cited by 11 publications

(15 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…45 Meanwhile, SDF showed the same action in less time with a high amount of fluoride and silver halt active carious lesions and provide antibacterial action. Our findings agree with another study, 41 which compared the remineralizing and antibacterial effect of SDF and propolis. It was found that SDF had superior antibacterial and remineralizing ability, which was attributed to the concentration of active ingredients in SDF that reach up to 38%, as opposed to propolis fluoride which only contains 10% active material.…”

Section: Discussionsupporting

confidence: 93%

“…After 6 weeks the propolis group had increased mineral density of carious dentin mainly due to flavonoids present in it that can induce reparative dentin through upregulation of growth factors (TGF-β1) which interact with the extracellular matrix resulting in collagen formation. 41,42 Furthermore, flavonoids can inhibit microorganisms by denaturing proteins and nucleic acids. Hindering of bacteria will decrease demineralization and help in mineral precipitation.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of the remineralization and antibacterial effect of natural versus synthetic materials on deep carious dentin: A randomized controlled trial

Anani¹,

Elasser²,

Niazy³

et al. 2023

Dent. Med. Probl.

View full text Add to dashboard Cite

Background.A variety of natural and synthetic agents have recently been used in clinical trials to arrest dentin caries.Objectives. The aim of the present study was to explore the remineralization and antibacterial effect of natural (propolis, hesperidin) vs. synthetic (silver diamine fluoride (SDF)) agents on deep carious dentin. Material and methods.A total of 64 human molar teeth with Class I caries were randomly distributed into 4 groups: control group; propolis group; hesperidin group; and SDF group. The cavities were prepared using the stepwise caries removal technique, and then covered with the materials to be tested. The samples were taken from the carious lesions before and after treatment to evaluate the antibacterial effect. Then, the teeth were restored with a glass ionomer cement (GIC). Digital X-rays were taken to assess remineralization and the antibacterial effect after 6 and 12 weeks.Results. The highest value of radiodensity was observed in the propolis group (46.44 ±9.65 HU), while the lowest value was noted in the hesperidin group (12.62 ±5.86 HU). The bacterial count in the propolis group was 1,280.00 ±1,480.54 CFU/mL at baseline, which was not significantly higher than the value measured after 6 weeks (574.00 ±642.48 CFU/mL; p = 0.153), whereas in the hesperidin group, the mean value of the bacterial count at baseline (3,166.67 ±1,940.79) was not much higher as compared to the value obtained at 6 weeks (2,983.33 ±1,705.77) (p = 0.150). Conclusions.In comparison with SDF, propolis and hesperidin agents showed promising effects in terms of remineralization of carious dental tissue and hindering the progression of caries.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Evaluation of the remineralization and antibacterial effect of natural versus synthetic materials on deep carious dentin: A randomized controlled trial

Anani¹,

Elasser²,

Niazy³

et al. 2023

Dent. Med. Probl.

View full text Add to dashboard Cite

show abstract

“…The results of the current study revealed that after SCT, dentin degradation might increase, due to MMP-8. Other authors described a lower expression of MMP-8 in visit 2 after SCT and sealing caries altered dentin [48], as well as a slight decrease of MMP-8 activity with "increasing depth of dentinal caries lesions" [47]. Tissue inhibitors of metalloproteinases, which were also considered within the present investigation, are known to control MMP activity, thus limiting dentin breakdown can be assumed [49,50].…”

Section: Discussionsupporting

confidence: 50%

Detection of Inflammatory and Homeostasis Biomarkers after Selective Removal of Carious Dentin—An In Vivo Feasibility Study

Schmidt

Hübler

Krohn

et al. 2021

JCM

View full text Add to dashboard Cite

Deep carious dentin lesions induce an immune reaction within the pulp-dentin complex, leading to the release of cytokines, which might be suitable biomarkers in pulp diagnostics. This in vivo feasibility study determines the concentration of different cytokines after selective removal of carious infected dentin (SCR). In our methodology, paired samples are obtained from 21 patients—each of them with two deep carious lesions at posterior teeth without clinical symptoms. After SCR, lesions are randomly assigned to treatment strategy: Group 1 (11 patients): Carious dentin is covered either with BiodentineTM (n = 11) or gutta-percha (n = 11) before using the adhesive OptibondTM FL. Group 2 (10 patients): The adhesives ClearfilTM SE Protect Bond (n = 10) or ClearfilTM SE Bond 2 (n = 10) are directly applied. Prepared cavities are rinsed with phosphate buffered saline containing 0.05% Tween 20 (10X) for five minutes immediately after SCR (visit 1) and eight weeks later (visit 2). Rinsing liquid is regained. Concentrations of IL-1β, IL-6, IL-10, C-reactive protein (CRP), TNF-α, IFN-γ, TIMP-1, -2, and MMP-7, -8, -9 are assessed by customized multiplex assays, evaluated with fluorescence analyzer. Non-parametric statistical analysis (Wilcoxon, Mann–Whitney U Test, p < 0.05) is performed (SPSS 25). Our results show that concentrations of CRP, IL-1β, IL-6, TIMP-1, -2, and MMPs were detectable. Median concentrations of CRP, IL-1β und IL-6 were significantly higher in visit 1 (304.9, 107.4, 3.8 pg/mL), compared to visit 2 (67.8, 2.3, 0.0 pg/mL; pi < 0.001). The study revealed that the non-invasive determination of cytokines from prepared dental cavities is possible.

show abstract

“…The importance of good dentine sealing on inhibiting MMP activity has previously been addressed in the literature [ 44 , 45 ]. Low pH caused by the natural caries process (bacterial acids) or acid-etching can result in activation of the MMP enzymes.…”

Section: Discussionmentioning

confidence: 99%

Renewal MI Dental Composite Etch and Seal Properties

et al. 2022

View full text Add to dashboard Cite

This study’s aim was to assess whether the Renewal MI composite can self-etch enamel, seal sound cavities, and stabilize demineralized dentine. Etching was assessed using scanning electron microscopy (SEM). Cavity sealing was quantified using the ISO-11405 dye microleakage test. Demineralized dentine stabilization was evaluated by visualizing resin tag formation, enzyme activity and mineral precipitation at the adhesion interface. Renewal MI provided a mild etching of sound enamel in comparison with 37% phosphoric acid. It provided a comparable seal of sound cavities to Z250/Scotchbond Universal adhesive and a superior seal to Activa, Fuji IX and Fuji II LC. With demineralized dentine, Renewal MI formed 300–400 µm resin tags covering 63% of the adhesion interface compared with 55 and 39% for Z250/Scotchbond and Activa. Fuji IX and Fuji II LC formed no resin tags. A higher tag percentage correlated with lower surface enzyme activity. Unlike Activa and Fuji II LC, Renewal MI promoted mineral precipitation from simulated body fluid, occluding adjacent dentinal tubules within 6 months. These novel etching and sealing properties may facilitate Renewal MI’s application in minimally invasive dentistry.

show abstract

Effect of sealing infected dentin with glass ionomer cement on the abundance and localization of MMP‐2, MMP‐8, and MMP‐9 in young permanent molars in vivo

Abstract: The sealing of infected carious dentin in young permanent molars reduced the expression of MMP-8, which is consistent with the initial remodeling process of the dentin matrix.

Cited by 11 publications

References 35 publications

Evaluation of the remineralization and antibacterial effect of natural versus synthetic materials on deep carious dentin: A randomized controlled trial

Evaluation of the remineralization and antibacterial effect of natural versus synthetic materials on deep carious dentin: A randomized controlled trial

Detection of Inflammatory and Homeostasis Biomarkers after Selective Removal of Carious Dentin—An In Vivo Feasibility Study

Renewal MI Dental Composite Etch and Seal Properties

Contact Info

Product

Resources

About